A novel target and pH dual-activatable fluorescent probe for precisely detecting hypochlorite in lysosomes

Shi, Lei; Yang, Sheng; Hong, Haojia; Li, Yong; Yu, Huijuan; Shao, Guang; Zhang, Kai; Gong, Shuling

doi:10.1016/j.aca.2019.10.004

Cited by 33 publications

(5 citation statements)

References 45 publications

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“…All results show that the probe is a potential near-infrared fluorescence imaging tool. Similar to the above-mentioned probe design strategy, Shi et al designed a fluorescent sensor 24 [ 51 ] that can target lysosomes with N,N -dimethylthiocarbamate (DMTC) as the reaction site. The legume fluorophore is the response group.…”

Section: Classification Of Small Molecule Fluorescent Probes For Detecting Hypochlorous Acid/hypochloritementioning

confidence: 99%

“…It can detect exogenous and endogenous HClO in lysosomes of living cells. Hou et al [51] developed a new probe 25 (Figure 25) for detecting ClO − , which combines coumarin with benzothiazine and adds a piperazine unit to improve cell membrane permeability and solubility. The sulfur atom in the probe can be oxidized by ClO − and emit green fluorescence.…”

Section: Fluorescent Probes Based On Coumarinmentioning

confidence: 99%

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Research Progress of Small Molecule Fluorescent Probes for Detecting Hypochlorite

Song

Yuan

et al. 2021

Sensors

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Hypochlorous acid (HOCl) generates from the reaction between hydrogen peroxide and chloride ions via myeloperoxidase (MPO)-mediated in vivo. As very important reactive oxygen species (ROS), hypochlorous acid (HOCl)/hypochlorite (OCl−) play a crucial role in a variety of physiological and pathological processes. However, excessive or misplaced production of HOCl/OCl− can cause variety of tissue damage and human diseases. Therefore, rapid, sensitive, and selective detection of OCl− is very important. In recent years, the fluorescent probe method for detecting hypochlorous acid has been developed rapidly due to its simple operation, low toxicity, high sensitivity, and high selectivity. In this review, the progress of recently discovered fluorescent probes for the detection of hypochlorous acid was summarized with the aim to provide useful information for further design of better fluorescent probes.

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Section: Classification Of Small Molecule Fluorescent Probes For Detecting Hypochlorous Acid/hypochloritementioning

confidence: 99%

Section: Fluorescent Probes Based On Coumarinmentioning

confidence: 99%

Research Progress of Small Molecule Fluorescent Probes for Detecting Hypochlorite

Song

Yuan

et al. 2021

Sensors

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“…Common fluorescent probes such as coumarin, 3 rhodamine, 4 luciferin, 5 pyrene, 6 naphthalene, 7,8 and boron dipyrromethene (BODIPY) derivatives 9,10 have been selected as excellent near-infrared fluorophores. The main recognition groups of ClO − include chalcogenides (S, Se, and Te), 11 acylhydrazine, 12 hydroxylamine, 13 hydrazine, 14 Schiff bases, 15,16 unsaturated bonds, 17 anisidine, 18 dimethylthiocarbamate 19 and p -methoxyphenol. 10 Along with these strategies, many efforts have been devoted to detecting ClO − by introducing p -methoxyphenol in the past few years (Chart 1).…”

Section: Introductionmentioning

confidence: 99%

A dicyanoisophorone-based fluorescent probe for hypochlorite with a fast response and its applications in bioimaging

Chan

Xue

et al. 2022

Anal. Methods

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“…Fluorescent probes emerged as an indispensable frontier technique for tracking endogenous biological reactive species or visualizing the morphology of the microstructure with high spatiotemporal resolution. , Currently, numerous HClO fluorescence probes were developed by employing a wide range of HClO-specific recognition sites, including p -amino/methoxyphenol, , sulfur/selenium-containing moieties, − oxime, and others, − and the key sensing parameters of these probes are summarized in Table S1. Even though the majority of these probes are capable of imaging endogenous HClO levels, no attempts, to the best of our knowledge, for long-term continuous imaging of endogenous HClO bursts have been reported.…”

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confidence: 99%

De Novo Design of a Highly Stable Ratiometric Probe for Long-Term Continuous Imaging of Endogenous HClO Burst

Chen,

Xu,

Xing

et al. 2024

Anal. Chem.

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Long-term continuous imaging of endogenous HClO burst is of great importance for the elucidation of various physiological or pathological processes. However, most of the currently reported HClO probes have failed to achieve this goal due to their insufficient photobleaching resistance under a laser source. Herein, a highly stable ratiometric probe, HFTC-HClO 1, which is capable of continuously monitoring endogenous HClO burst over a long period of time, has been judiciously developed. Briefly, the de novo development of HFTC-HClO 1 mainly involved three main steps: (1) novel coumarins (HFTC 1−5) were designed and synthesized; (2) the most stable scaffold, HFTC 3, was selected through dye screening and cell imaging validation; and (3) based on HFTC 3, three candidate HClO probes were constructed, and HFTC-HClO 1 was finally selected due to its superior sensing properties toward HClO. Furthermore, HFTC-HClO 1 can quantitatively measure HClO levels in various real samples with excellent recovery (>90.4%), and the use of HFTC-HClO 1-coated test strips for qualitative analysis of HClO in real samples was also achieved. In addition, the application of HFTC-HClO 1 for longterm continuous monitoring of intracellular HClO burst was successfully demonstrated. Significantly, HFTC-HClO 1 was able to visualize HClO generated in the rheumatoid arthritis mouse model.

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A novel target and pH dual-activatable fluorescent probe for precisely detecting hypochlorite in lysosomes

Cited by 33 publications

References 45 publications

Research Progress of Small Molecule Fluorescent Probes for Detecting Hypochlorite

Research Progress of Small Molecule Fluorescent Probes for Detecting Hypochlorite

A dicyanoisophorone-based fluorescent probe for hypochlorite with a fast response and its applications in bioimaging

De Novo Design of a Highly Stable Ratiometric Probe for Long-Term Continuous Imaging of Endogenous HClO Burst

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