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A novel “donor–π–acceptor” type fluorescent probe has been prepared for the detection of Cys. This probe shows great fluorescent performance and obvious response to Cys, and has been successfully applied in visualizing Cys in living cells.

A new fluorescent probe based on rhodamine and naphthalimide was synthesized for the discrimination of hypochlorite anion. Upon addition of NaClO, the emission intensities ratio (I576 nm/I528 nm) of the probe 1 increased quickly accompanied with the obvious change of color. The probe 1 also exhibited highly selectivity and fast response to hypochlorite anion. Furthermore, the newly proposed probe has been applied for natural water samples, and a satisfied result was obtained.

It is extremely important to develop selective and sensitive fluorescent probes for the detection of hypochlorite (HClO/ClO−). Herein, two ESIPT‐based fluorescent probes (BS1 and BS2) were designed and prepared for the fast determination of HClO. The probes exhibited excellent specificity towards HClO and showed the rapid and huge fluorescent responses. Moreover, the probes BS1 and BS2 could sensitively detect HClO in the range of 0–100 μM with the low detection limits of 87 and 106 nM, especially. Besides, the success of practical application in water samples and test strips suggested that the synthetic probes may be effective tools for the detection of hypochlorite in environmental samples.