A novel technique for the preparation of transport-active membrane vesicles from Pseudomonas aeruginosa: Observations on gluconate transport

Stinnett, J. D.; Guymon, L F; Eagon, R. G.

doi:10.1016/0006-291x(73)90985-6

Cited by 37 publications

(27 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results confirm and extend earlier observations of GDH-mediated transport of solutes in membrane vesicles from P . aeruginosa (Stinnett et al, 1973) and Escherichia coli .…”

Section: Discussionmentioning

confidence: 99%

Glucose-dehydrogenase-mediated Solute Transport and ATP Synthesis in Acinetobacter calcoaceticus

Schie¹,

Pronk²,

Hellingwerf³

et al. 1987

Microbiology

View full text Add to dashboard Cite

Evidence is presented that in Acinetobacter calcoaceticus oxidation of glucose to gluconate by the periplasmic quinoprotein glucose dehydrogenase (EC 1 . 1 .99.17) leads to energy conservation.Membrane vesicles prepared from cells grown in carbon-limited chemostat culture exhibited (1) a high rate of glucose-dependent oxygen consumption and gluconate production, (2) glucosemediated cytochrome reduction, (3) uncoupler sensitive, glucose-dependent generation of a membrane potential and (4) glucose-driven accumulation of amino acids. Furthermore, oxidation of glucose to gluconate by whole cells was associated with ATP synthesis. These results confirm and extend previous observations that periplasmic glucose oxidation can act as a driving force for energy-requiring processes. It is therefore concluded that the incomplete oxidation of glucose by bacteria may serve as an auxiliary energy-generating system.

show abstract

“…These results confirm and extend earlier observations of GDH-mediated transport of solutes in membrane vesicles from P . aeruginosa (Stinnett et al, 1973) and Escherichia coli .…”

Section: Discussionmentioning

confidence: 99%

Glucose-dehydrogenase-mediated Solute Transport and ATP Synthesis in Acinetobacter calcoaceticus

Schie¹,

Pronk²,

Hellingwerf³

et al. 1987

Microbiology

View full text Add to dashboard Cite

show abstract

“…Neither methionine-transporting membrane vesicles, prepared by the method of Stinnett, Guymon & Eagon (1973), nor the corresponding cytoplasmic fraction were able to demethiolate methionine. The relationship of methionine transport to its demethiolation was therefore studied using intact organisms.…”

Section: R E S U L T Smentioning

confidence: 93%

The Relationship between Methionine Uptake and Demethiolation in a Methionine-utilizing Mutant of Pseudomonas fluorescens UK1

Laakso¹

1976

Journal of General Microbiology

View full text Add to dashboard Cite

“…Lysine transport by vesicles prepared either by the method of Kaback (1971) or by that of Stinnett et al (1973) was assayed using, as electron donors, ascorbate plus phenazine methosulphate (Konings & Freese, 1972) or malate plus FAD (Mantsala et aZ., 1974;Stinnett et a/., 1973).…”

Section: W E I L L -T H~v E N E T a N D O T H E R Smentioning

confidence: 99%

“…We attempted to measure lysine transport into vesicles prepared by the methods of Stinnett et al (1973) and Kaback (1971); the former method avoids the use of Tris/HCl buffer which provokes lysis of Pseudonzonas (Gilleland et al, 1973). However, these attempts were unsuccessful, possibly because lysine transport was shock-sensitive, as in E. coli (Rosen, 1971).…”

Section: R E S U L T Smentioning

confidence: 99%

Lysine Transport Systems in Pseudomonas in Relation to Their Physiological Function

Weill-Thévnet¹,

Hermann²,

Vandecasteele³

1979

Journal of General Microbiology

View full text Add to dashboard Cite

Pseudomonas putida, which can use lysine as a carbon and energy source, has two active transport systems for lysine -a high affinity system, inhibited by arginine and ornithine, and a low affinity system. Lysine-grown organisms had a higher transport activity than succinate-grown organisms but this higher activity was probably not the result of induction by lysine. Pseudomonas acidovorans, a species unable to degrade lysine, has a single high affinity active transport system specific for lysine; this transport system has a physiological role in the maintenance of the internal concentration of free lysine. I N T R O D U C T I O NThe transport of basic amino acids has been studied in detail in Escherichia coli. Lysine uptake is mediated in this organism by means of two transport systems. One system, with a K, of 1 0 ,~~~ is specific for lysine alone. The other system has a high affinity for lysine (K, 0.5 ,UM) but also transports arginine and ornithine (LAO system). It involves a binding protein which is released by osmotic shock (Rosen, 1971). The physiological role of these transport systems is uncertain. The aim of the present work was to obtain such information from a comparative study of lysine transport in Pseudomonasputida which can use lysine as a carbon source and P. acidovorans which does not degrade lysine (Stanier et al., 1966). M E T H O D SOrganisms and growth. Pseudomonas acidovorans L6, P. putida T1 and P. putida T1 L12, a mutant of strain T1 unable to degrade lysine, were isolated in this laboratory and have been described previously . They were grown at 30 "C in Fernbach flasks in the mineral medium described by Robinson (1 964) containing 50 mM-sodium succinate or alternative carbon source, as indicated in the text.Measurement of transport. The organisms were harvested in the exponential growth phase (0-3 to 0.6 absorbance units) by centrifuging at 20 "C for 10 min at 6000 g, washed once in mineral medium and resuspended in the same medium to a concentration of 0.1 mg dry wt organisms ml-l. The mineral medium used was the same as that used for growth except that ammonium salts were omitted to prevent growth during measurement of lysine transport. The suspension was shaken for 10 min at 30 "C before use. When chloramphenicol was used, it was added at the beginning of this preincubation period which was extended to 30 min. ~-[U-~~C]Lysine was then added at the specified concentration. At intervals, 1 ml samples were withdrawn with an Eppendorf pipette, transferred to HAWP Millipore filters (pore size 0.45 pm) to which 2 ml mineral medium had previously been added, and immediately vacuum filtered. The filters were washed with 10 ml mineral medium at room temperature, then transferred to scintillation vials and dried (30 min under an infrared lamp). They were then immersed in 10 ml scintillation mixture [containing 5 g 2,5-diphenyloxazole and 0.5 g 1,4-di-2-(methylstyryl)benzene in 1 1 toluene], and radioactivity was determined with a Packard 3320 liquid scintillation spectrometer. Counting efficien...

show abstract

A novel technique for the preparation of transport-active membrane vesicles from Pseudomonas aeruginosa: Observations on gluconate transport

Cited by 37 publications

References 11 publications

Glucose-dehydrogenase-mediated Solute Transport and ATP Synthesis in Acinetobacter calcoaceticus

Glucose-dehydrogenase-mediated Solute Transport and ATP Synthesis in Acinetobacter calcoaceticus

The Relationship between Methionine Uptake and Demethiolation in a Methionine-utilizing Mutant of Pseudomonas fluorescens UK1

Lysine Transport Systems in Pseudomonas in Relation to Their Physiological Function

Contact Info

Product

Resources

About