A novel terbium (III) and aptamer-based probe for label-free detection of three fluoroquinolones in honey and water samples

Chen, Jin'ai; Jin, Yuting; Ren, Taotao; Wang, Shuo; Wang, Xianghong; Zhang, Fuyuan; Tang, Yiwei

doi:10.1016/j.foodchem.2022.132751

Cited by 25 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The IC50 of the developed fluorescence ELISA was 2.43 μg L -1 , and the IC15 of the developed fluorescent ELISA was 0.20 μg L -1 , which was about 12-fold lower than the IC15 of the conventional ELISA (2.50 μg L -1 ), with an IC50 of 21.98 μg L -1 . As shown in Table 1, the analytical feature of our established method was compared to several immunoassays for NOR determination (Cheng et al, 2022;Han et al, 2021;Han et al, 2022;Hu, Gao, Han, & Yang, 2020;Jin et al, 2022;Liu et al, 2021;Wu et al, 2017;Yu et al, 2018;Zong et al, 2019). The developed method showed ideal LOD and detection time.…”

Section: Construction Of Qds-based Fluorescent Quenching Elisamentioning

confidence: 99%

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

et al. 2023

Preprint

View full text Add to dashboard Cite

A highly sensitive and reliable fluorescence immunoassay based on the inner-filter effects (IFE) of quantum dots (QDs) was developed for the sensitive detection of norfloxacin (NOR) in animal-origin foods. Briefly, the enzymatic formation of horseradish peroxidase in conventional ELISA effectively quenched the fluorescence of QDs through IFE, and then the absorption signal of conventional ELISA was transformed to fluorescent signals, resulting in a higher sensitivity for NOR detection in animal-origin foods.The detection limit of NOR in this developed method was 0.20 µg L− 1 in standard solution, which was 12-fold more sensitive than conventional ELISA (2.50 µg L− 1). The results of specificity analysis revealed that the proposed method could not only be used for the detection of NOR, but also for the other 9 structural analogues. The recovery rates of NOR-spiked animal-origin foods ranged from 88.11–108.16%, with CV values of 1.95%-18.03%. In addition, the established method had good consistency with the commercial ELISA kits and HPLC, indicating the accuracy of the developed method for the detection of NOR in real samples. In conclusion, the established fluorescence immunoassay was sensitive and facile, and could be alternatively used for the determination of NOR residues in animal-origin foods.

show abstract

Section: Construction Of Qds-based Fluorescent Quenching Elisamentioning

confidence: 99%

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…However, there are many types of antibiotics in foods, and most antibiotic residues in complicated samples are measured in the PPM range. Therefore, the detection of trace antibiotics in these complex substrates is urgently required [9,10].…”

Section: Introductionmentioning

confidence: 99%

Efficient Sequential Detection of Two Antibiotics Using a Fiber-Optic Surface Plasmon Resonance Sensor

Zhao,

Yin,

Xiao

et al. 2024

Sensors

View full text Add to dashboard Cite

Antibiotic residues have become a worldwide public safety issue. It is vital to detect multiple antibiotics simultaneously using sensors. A new and efficient method is proposed for the combined detection of two antibiotics (enrofloxacin (Enro) and ciprofloxacin (Cip)) in milk using surface plasmon resonance (SPR) sensors. Based on the principle of immunosuppression, two antibiotic antigens (for Enro and Cip) were immobilized on an optical fiber surface with conjugates of bovine serum albumin using dopamine (DA) polymerization. Each single antigen was bound to its corresponding antibody to derive standard curves for Enro and Cip. The fiber-optic sensor’s sensitivity was 2900 nm/RIU. Detection limits were calculated to be 1.20 ng/mL for Enro and 0.81 ng/mL for Cip. The actual system’s recovery rate was obtained by testing Enro and Cip in milk samples; enrofloxacin’s and ciprofloxacin’s mean recoveries from the milk samples were 96.46–120.46% and 96.74–126.9%, respectively. In addition, several different regeneration solutions were tested to analyze the two target analytes’ regeneration ability; NaOH and Gly-HCl solutions were found to have the best regeneration ability.

show abstract

Fabrication of highly fluorinated porphyrin-based covalent organic frameworks decorated Fe3O4 nanospheres for magnetic solid phase extraction of fluoroquinolones

et al. 2022

View full text Add to dashboard Cite

A novel terbium (III) and aptamer-based probe for label-free detection of three fluoroquinolones in honey and water samples

Cited by 25 publications

References 55 publications

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

A novel and sensitive fluorescence immunoassay on the basis of the inner-filter effects of quantum dots for the determination of norfloxacin in animal-origin foods

Efficient Sequential Detection of Two Antibiotics Using a Fiber-Optic Surface Plasmon Resonance Sensor

Fabrication of highly fluorinated porphyrin-based covalent organic frameworks decorated Fe3O4 nanospheres for magnetic solid phase extraction of fluoroquinolones

Contact Info

Product

Resources

About