Sediment and flow depth monitoring in sewers is important for informing flow models and for predicting and mitigating against sewer blockage formation and surcharge. In this study, a novel sensor based on conductance measurement has been developed and tested under a laboratory environment and validated by a finite-element model. The relative conductance is measured between pairs of adjacent electrodes to provide a conductance profile along the sensor length. A piecewise linear relationship between conductance and electrode length was derived and the interface positions between sediment, water, and air can be determined from the profile. The results demonstrated that the root mean square error of the model and the measured interface level are within 1.4% and 2.6% of sensor’s measurement range. An error distribution of interface height shows that all anticipated errors are within the resolution of the electrode length increments. Furthermore, it was found that the conductivity of the measured medium is proportional to the gradient of the linear relationship of conductance and electrode length. It could therefore prove a valuable new tool for the accurate quantification of sediment and flow levels in sewer conduits, coastal environments, drainage systems for transport networks, and other industrial or academic applications.