A novel tissue-engineered bone graft composed of silicon-substituted calcium phosphate, autogenous fine particulate bone powder and BMSCs promotes posterolateral spinal fusion in rabbits

Cui, Li-Huang; Xiang, Shou-Yang; Chen, DeChun; Fu, Rui; Zhang, Xin; Chen, JingTao; Wang, XinTao

doi:10.1016/j.jot.2020.06.003

Cited by 23 publications

(16 citation statements)

References 39 publications

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“…Spatial changes in bony architecture can be observed, and the amount of newly formed bone tissue can be calculated by Micro-CT, which enables comparisons between different interventions. Micro-CT has detected increases in bone mineral density (BMD), percent bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and decreases in trabecular separation (Tb.Sp) in a series of studies promoting spinal fusion [24][25][26][27]. In this study, BV/TV and Tb.N were greater in group B compared to group A, which is in line with previous research [24].…”

Section: Discussionmentioning

confidence: 89%

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

Kang

Liu

Wang

et al. 2021

BMC Musculoskelet Disord

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Background Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery. Methods The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and 8 weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis. Results Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P < 0.05). The BV/TV (B: 42.20 ± 10.50 vs. A: 29.02 ± 3.25, P < 0.05) and Tb.N (B: 4.66 ± 1.23 vs. A: 1.97 ± 0.40, P < 0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10 ± 0.04 vs. A: 0.15 ± 0.02, P < 0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72 ± 12.80 vs. A: 12.36 ± 16.93, P < 0.05). Conclusion A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion.

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Section: Discussionmentioning

confidence: 89%

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

Kang

Liu

Wang

et al. 2021

BMC Musculoskelet Disord

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

A Novel Rat Model of Interbody Fusion based on Anterior Lumbar Corpectomy and Fusion (ALCF)

Kang

Liu

Wang

et al. 2021

Preprint

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Background: Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery.Methods: The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and eight weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis.Results: Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P<0.05). The BV/TV (B: 42.20±10.50 vs. A: 29.02±3.25, P<0.05) and Tb.N (B: 4.66±1.23 vs. A: 1.97±0.40, P<0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10±0.04 vs. A: 0.15±0.02, P<0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72±12.80 vs. A: 12.36±16.93, P<0.05).Conclusion: A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion.

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“…One is to connect the cells directly to a scaffold, and the other is to form a cell sheet in vitro to improve the adhesion rate, and then use the sheet directly or attach it to a scaffold [ 59 ]. The cells applied for tissue engineering of periosteum include PDCs [ 60 ], skeletal stem cells [ 61 ], BMSCs [ 62 ], adipose mesenchymal stem cells (AMSCs), and dental pulp stem cells [ 63 ] ( Fig. 4 ).…”

Section: Composition and Application Of Tissue-engineered Periosteummentioning

confidence: 99%

Periosteum and development of the tissue-engineered periosteum for guided bone regeneration

Zhang

Wang

Yang

et al. 2022

Journal of Orthopaedic Translation

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A novel tissue-engineered bone graft composed of silicon-substituted calcium phosphate, autogenous fine particulate bone powder and BMSCs promotes posterolateral spinal fusion in rabbits

Cited by 23 publications

References 39 publications

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

A Novel Rat Model of Interbody Fusion based on Anterior Lumbar Corpectomy and Fusion (ALCF)

Periosteum and development of the tissue-engineered periosteum for guided bone regeneration

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