A novel tool for clinical diagnosis of allergy operating a microfluidic immunoaffinity basophil activation test technique

Aljadi, Zenib; Kalm, Frida; Nilsson, Caroline; Winqvist, Ola; Russom, Aman; Lundahl, Joachim; Nopp, Anna

doi:10.1016/j.clim.2019.108268

Cited by 11 publications

(14 citation statements)

References 174 publications

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“…This observation is also of importance for the miBAT technique as a high background in combination with a lower activation with anti-FcϵRI and relevant allergens in chip (miBAT) has been observed compared with flow cytometry-based BAT. 11 Cross-linking of CD203c and activation would therefore also be essential conditions to investigate further and IgE-independent activation in miBAT. In contrast to adhesion molecule cross-linking, IL-3 and IL-33 exposure followed by IgE-independent degranulation via fMLP increases the CD63 expression.…”

Section: Discussionmentioning

confidence: 99%

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Adhesion molecule cross‐linking and cytokine exposure modulate IgE‐ and non‐IgE‐dependent basophil activation

et al. 2020

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The knowledge of the different roles of basophils have emerged to include distinct functions for both innate and adaptive immunity. To accomplish these functions, the basophil depends on the capability to migrate from blood to different extravascular tissues e.g. lungs and lymphoid tissues. Basophils are well known for their role in allergic inflammation, which make them potential targets in allergy diagnostics such as the basophil activation test (BAT) and the further developed microfluidic immunoaffinity basophil activation test (miBAT). We aimed to investigate how basophil activation is regulated by cytokines and crosslinking of adhesion molecules to gain knowledge of basophil regulation in the in vivo recruitment process and to enable future optimization of the miBAT. We analyzed the expression of adhesion and activation markers on basophils from healthy blood donors using flow cytometry. The CD63-expression on basophils was stable after flowing through a microfluidic chip. However, crosslinking of CD203c, CD62L, CD11b and CD49d induced a slight but significant upregulation. To mimic in vivo activation, CD62L and CD49d were crosslinked followed by IgE-dependent and IgE-independent activation. IgEdependent activation (anti-IgE) after prior crosslinking of adhesion molecules resulted in reduced CD63-expression compared to anti-IgE activation alone. In addition, IL-3 and IL-33 priming increased the CD63-expression after IgE-independent activation (fMLP). Together, our data indicate that mechanisms that are potentially operational both in the microfluidic chip environment and in vivo during basophil adhesion and transmigration might impact basophil degranulation procedures such as anaphylactic degranulation and piecemeal degranulation and hence the basophil immune regulatory function.

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Section: Discussionmentioning

confidence: 99%

“…11 However, a challenge related to the miBAT is the background expression of CD63 in non-activated chip-captured basophils, which results in a more narrow analysis range. 11 To enable further improvement of the technique, this matter needs to be addressed.…”

Section: Introductionmentioning

confidence: 99%

Adhesion molecule cross‐linking and cytokine exposure modulate IgE‐ and non‐IgE‐dependent basophil activation

et al. 2020

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“…After activation, these cells degranulate and release different immune modulators, such as histamine, by cross-linking allergens to IgE-antibodies ( Fig. 5) [53].…”

Section: Rhinologymentioning

confidence: 99%

Prospects and Opportunities for Microsystems and Microfluidic Devices in the Field of Otorhinolaryngology

Hwang

Gonzalez-Suarez

Stybayeva

et al. 2021

Clin Exp Otorhinolaryngol

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Microfluidic systems can be used to control picoliter to microliter volumes in ways not possible with other methods of fluid handling. In recent years, the field of microfluidics has grown rapidly, with microfluidic devices offering possibilities to impact biology and medicine. Microfluidic devices populated with human cells have the potential to mimic the physiological functions of tissues and organs in a three-dimensional microenvironment and enable the study of mechanisms of human diseases, drug discovery and the practice of personalized medicine. In the field of otorhinolaryngology, various types of microfluidic systems have already been introduced to study organ physiology, diagnose diseases, and evaluate therapeutic efficacy. Therefore, microfluidic technologies can be implemented at all levels of otorhinolaryngology. This review is intended to promote understanding of microfluidic properties and introduce the recent literature on application of microfluidic-related devices in the field of otorhinolaryngology.

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“…58 Validation of a chip-based microfluidic immuno-affinity BAT (miBAT) was compared to conventional BAT. 59 The…”

Section: New Approaches For Diagnosismentioning

confidence: 99%

“…The nanofluidic assay (NFA) abioSCOPE ® allows accurate quantification of IgE at picomolar range within 5 minutes at the point‐of‐care 58 . Validation of a chip‐based microfluidic immuno‐affinity BAT (miBAT) was compared to conventional BAT 59 . The principle of miBAT is an initial capturing of basophils from patients’ whole blood via mobilized anti‐CD203c antibodies followed by the activation with a relevant allergen.…”

Section: Diagnostic Approachesmentioning

confidence: 99%

Advances and novel developments in molecular allergology

Üzülmez

Kalic

Breiteneder

2020

Allergy

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New technologies are changing the way research is performed in many areas of molecular allergology. 1 Recombinant (r) allergens have influenced the development of allergy diagnosis and allergen-specific immunotherapy (AIT) for over three decades. 2 Although the gold rush of allergen discovery is over, gaps in our knowledge of structures, cross-reactivities, and conformational epitopes are being constantly filled. 2,3

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A novel tool for clinical diagnosis of allergy operating a microfluidic immunoaffinity basophil activation test technique

Cited by 11 publications

References 174 publications

Adhesion molecule cross‐linking and cytokine exposure modulate IgE‐ and non‐IgE‐dependent basophil activation

Adhesion molecule cross‐linking and cytokine exposure modulate IgE‐ and non‐IgE‐dependent basophil activation

Prospects and Opportunities for Microsystems and Microfluidic Devices in the Field of Otorhinolaryngology

Advances and novel developments in molecular allergology

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