A novel type of VP4 carried by a porcine rotavirus strain

Liprandi, Ferdinando; Gerder, Marlene; Bastidas, Zoleida; López, J. A.; Pujol, Flor H.; Ludert, Juan E.; Joelsson, Daniel B.; Ciarlet, Max

doi:10.1016/s0042-6822(03)00534-8

Cited by 67 publications

(45 citation statements)

References 33 publications

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“…In addition, BLAST search (http://www.ncbi.nlm.nih.gov) confirmed the genetic relatedness of OVR762 with P [14] strains of the VP4 gene (85-92%) and its deduced protein sequence (93-97%) as well (data not shown). Since it has been established that rotavirus strains that exhibit a VP4 aa identity of approximately C89% belong to the same P genotype [32,33], our results indicate that the Spanish OVR762 strain belongs to the P [14] genotype.…”

Section: Analysis Of the Electropherotype (E-type)supporting

confidence: 51%

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

Ciarlet

Hoffmann

Lorusso³

et al. 2008

Virus Genes

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An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95-98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

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Section: Analysis Of the Electropherotype (E-type)supporting

confidence: 51%

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

Ciarlet

Hoffmann

Lorusso³

et al. 2008

Virus Genes

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“…In contrast, they showed somewhat lower levels of VP8* amino acid sequence identity with other P [13] reference strains, strains MDR-13, JP35-7, JP13-3, ICB2212, ICB2219, and A46, ranging from 79.2% to 83.7% amino acid identity for CMP178 and 79.2% to 83.2% amino acid identity for CMP213. Additionally, they also showed lower levels of amino acid sequence identity with P [22] lapine rotavirus reference strains 160/01, 229/01, 308/01, and 3489/3, ranging from 77.9% to 81.0% for CMP178 and 78.4% to 81.0% for CMP213. Therefore, all eight isolates were considered to be P [13]-like viruses since they had the highest level of sequence identity with P [13] PoRV strains and revealed remarkably lower levels of amino acid sequence identity with a The GenBank accession numbers of VP4 genes are as indicated for the following strains: RF, U65924; SA11, M23188; RRV, M18736; RV-5, M32559; UK, M22306; Gottfried, M33516; OSU, X13190; Wa, M96825; K8, D90260; 69M, M60600; B223, D13394; H-2, L04638; MDR-13, L07886; PA169, D14724; Lp14, L11599; EB, U08419; 993-83, D16352; L338, D13399; 4F, L10359; EHP, U08424; Hg18, AF237665; 160/01, AF526374; A34, AY174094; TUCH, AY596189; Dhaka6, AY773004; 134/04-15, DQ061053; and CMP034, DQ534016.…”

Section: Resultsmentioning

confidence: 99%

“…To date, at least 15 G genotypes and 26 P genotypes have been identified from humans and a variety of animal species (5,22,26,28,30,35,36). Most recently, several groups of investigators have proposed a novel genotype, P[27], which was isolated from diarrheic piglets (18,25,38).…”

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confidence: 99%

Multiple Combinations of P[13]-Like Genotype with G3, G4, and G5 in Porcine Rotaviruses

Chan-it¹,

Khamrin

Saekhow

et al. 2008

J Clin Microbiol

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Epidemiological surveillance of porcine rotavirus (PoRV) strains was carried out in Chiang Mai Province, Thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by PCR. Of these, six were G3 and one was G4 and displayed a P-nontypeable genotype, while another isolate was both G and P nontypeable. Analysis of a partial VP4 gene of all eight P-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged to the same P genotype. Comparison of the amino acid sequences of two representative strains (namely, strains CMP178 and CMP213) with those of 27 other known P genotypes revealed a high degree of amino acid sequence identity with those of P Group A rotaviruses are the most important etiologic agents of severe diarrhea in young children and young animals worldwide. In developing countries, these severe diarrhea cases lead to an estimated 454,000 to 705,000 deaths annually among children under 5 years of age (32). Group A rotaviruses are members of the Reoviridae family with nonenveloped icosahedral particles. The mature virion is formed by three concentric layers of proteins that enclosed a genome of 11 segments of double-stranded RNA. Rotaviruses are classified according to the genetic and antigenic diversity of the two outer capsid proteins, VP4 and VP7. These proteins independently induce type-specific neutralizing antibodies and form the basis of the dual classification of group A rotaviruses into the P (protease sensitive) and the G (glycoprotein) serotypes, respectively (5, 17).Rotaviruses express extensive antigenic and genomic diversities. To date, at least 15 G genotypes and 26 P genotypes have been identified from humans and a variety of animal species (5,22,26,28,30,35,36). Most recently, several groups of investigators have proposed a novel genotype, P[27], which was isolated from diarrheic piglets (18,25,38). Generally, rotaviruses of the same G genotypes share at least 90 to 91% VP7 amino acid sequence identity (11,12,13,16). Rotavirus strains sharing Ն89% VP4 amino acid sequence identities are considered to belong to the same P genotype, while those sharing VP4 amino acid sequence identities of Ͻ89% belong to different genotypes (2, 5, 7). Moreover, the VP8* trypsin-cleavage product of VP4 coding for amino acids (aa) 13 to 250, including the greatest sequence divergent region (aa 71 to 204), correlates well with VP4 genotype specificity (20,21).Several epidemiological studies have demonstrated that among porcine rotaviruses (PoRVs), G3, G4, and G5, are the most common G genotypes and usually associate with the P[6] or P [7] genotype (5,23,39,40). In addition, other G and P types, such as G1, G2, G6, G8, G9, and G10 and P [13] (1,3,5,9,10,16,18,27,29,33,34,37,39,40,41). Accordingly, a comprehensive genotypic characterization of the rotavirus strains circulating in domestic animal populations, especially pigs, is important to define the extent of rotavirus diversity.Rotavirus strains bearing P[13] genotype spe...

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“…As both epitopes are important for host immunity, eliciting neutralizing antibodies, a dual classification system for rotaviruses is used. To date at least 15 G and 27 P genotypes have been characterized using molecular techniques (Estes, 2001;Hoshino et al, 2002;Khamrin et al, 2007;Liprandi et al, 2003;Martella et al, 2003Martella et al, , 2006aMartella et al, , 2007McNeal et al, 2005;Rahman et al, 2005;Rao et al, 2000;Steyer et al, 2007a). Some of these genotypes have been found only in certain animal species or in humans and appear to be host-restricted.…”

Section: Introductionmentioning

confidence: 99%

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer

Poljšak-Prijatelj

Barlič–Maganja

et al. 2008

Journal of General Virology

118

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A surveillance of human, porcine and bovine rotaviruses was carried out in Slovenia in 2004 and 2005. Stool samples were collected from a total of 406 pigs (373 from asymptomatic animals), 132 cattle (126 from asymptomatic animals) and 241 humans (all with diarrhoea), tested for group A rotaviruses using RT-PCR and analysed by sequencing. The aims of the study were to determine the incidence of asymptomatic rotavirus infection in animals, to look for evidence of zoonotic transmission and to detect reassortment among rotaviruses. The rates of asymptomatic shedding of rotaviruses in pigs and cattle were 18.0 % (67/373) and 4.0 % (5/126), respectively. Evidence for zoonotic transmission was detected in one human rotavirus strain, SI-MB6, with the G3P[6] genotype combination, as the nucleotide and predicted amino acid sequences of the VP6, VP7, VP8* and NSP4 genes of strain SI-MB6 and of porcine strains showed high nucleotide and amino acid sequence identity. Two porcine rotavirus strains carried VP7 of probable human origin, suggesting an interspecies reassortment event in the past.

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A novel type of VP4 carried by a porcine rotavirus strain

Cited by 67 publications

References 33 publications

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

Multiple Combinations of P[13]-Like Genotype with G3, G4, and G5 in Porcine Rotaviruses

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

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