A novel vibriophage vB_VhaS_PcB-1G capable of inhibiting virulent Vibrio harveyi pathogen

Wu, Liting; Tian, Yuan; Pang, Maoda; Yang, Zhenquan; Bao, Hongduo; Zhou, Yan; Sun, Lichang; Wang, Ran; Zhang, Hui

doi:10.1016/j.aquaculture.2021.736854

Cited by 17 publications

(16 citation statements)

References 52 publications

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“…Because of this, an increasing number of studies aiming to control vibriosis have been conducted by employing phages as therapeutic agents. To our knowledge, 21 bacteriophages have been previously isolated against V. harveyi, including 16 siphoviruses [61][62][63][64][65][66], 4 myoviruses [17,62,67,68], and 1 podovirus [69]. Two bacteriophages, VHML and Siphophage 1 VHS1, were found to be temperate [66,68], while the rest are considered to be lytic.…”

Section: Discussionmentioning

confidence: 99%

“…Vibrio phages have occasionally been associated with inducing virulence in their hosts [68,86], and hence a comprehensive profiling of their genomic traits is required before proceeding to therapeutic application. Only 4 of the 21 phages against V. harveyi that were isolated in previous studies have been sequenced and characterized genomically [17,63,86,87]. The genome sizes of these four phages vary between 48 and 286 kbp, including a jumbo bacteriophage Vb_VhaM_pir03.…”

Section: Discussionmentioning

confidence: 99%

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Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi

Droubogiannis

Katharios

2022

Pathogens

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Due to the emergence of multidrug-resistant bacteria, commonly known as “superbugs”, phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in aquaculture, resulting in huge economic and production losses. We isolated and fully characterized a novel bacteriophage, Vibrio phage Virtus, infecting V. harveyi strain VH2. Vibrio phage Virtus can infect a wide spectrum of Vibrio spp., including strains of V. harveyi, V. owensii, V. campbellii, V. parahaemolyticus, and V. mediterranei. It has a latent period of 40 min with an unusually high burst size of 3200 PFU/cell. Vibrio phage Virtus has a double-stranded DNA of 82,960 base pairs with 127 predicted open reading frames (ORFs). No virulence, antibiotic resistance, or integrase-encoding genes were detected. In vivo phage therapy trials in gilthead seabream, Sparus aurata, larvae demonstrated that Vibrio phage Virtus was able to significantly improve the survival of larvae for five days at a multiplicity of infection (MOI) of 10, which suggests that it can be an excellent candidate for phage therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi

Droubogiannis

Katharios

2022

Pathogens

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“…To evaluate pH stability of PVA23, aliquots of 1 mL stocked solution (10 9 PFU/mL) was treated at 37 • C for 2 h with 10 mL PBS buffers with varied pH values (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Then the double-layer agar method was used to calculate the phage titer.…”

Section: Determination Of Thermal and Ph Stability Of Phage Pva23mentioning

confidence: 99%

“…The use of phage therapy can be traced back to a century ago, and later on it was widely accepted, thanks to a panel of advantages over traditional antibiotic therapies [ 9 ]. Phage therapy has been considered as a potential alternative to antibiotic therapy in treating bacterial infections, and it had been successfully used to control several pathogenic infections in humans, and veterinary and agricultural settings [ 10 ]. Recently, some investigators have explored phage therapy for aquatic farming application as well as for bio-preservation use in food industries [ 8 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Genomic Analysis of a Bacteriophage with Potential in Lysing Vibrio alginolyticus

Zhao

et al. 2022

Viruses

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Vibrio alginolyticus is one of the major pathogens causing vibriosis to a variety of aquatic animals as well as bringing about severe food safety concerns. Nowadays, phage therapy has received increasing attention as an alternative to the antibiotics that have being limited for use in aquaculture industries. In this work, a potent bacteriophage, vB_ValM_PVA23 (PVA23), which efficiently infects pathogenic strains of V. alginolyticus, was isolated from sewage water and characterized by microbiological and genomic analyses. Based on the transmission electronic observation, the phage was characterized to be the Myoviridae family. It has a latent period of 10 min and a burst size of 203 PFUs/infected bacterium, and was stable over a broad pH range (5.0–11.0) and a wide temperature span (−80 °C to 60 °C), respectively. Genome sequencing results show that PVA23 has a 246,962-bp double-stranded DNA with a G + C content of 41.25%. The lab and plant shrimp farming trials demonstrated that phage preparation derived from PVA23 out-performed the chemical disinfectant iodine treatment in the prevention of V. alginolyticus propagation, and the phage application could rapidly yet significantly reduce the level of V. alginolyticus in the pond within 12 h, with negligible rebound observed. These results suggests that phage PVA23 has the potential to be used as an anti-V. alginolyticus agent in aquaculture industries.

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“…Phage SQ17 was tested against 20 strains by the spot test method (Wu et al, 2021) with some modifications in order to determine its host range. The bacterial strains used in this assay are listed in Table 1.…”

Section: Host Range Determinationmentioning

confidence: 99%

Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of Enterohemorrhagic Escherichia coli O157:H7 and Enterotoxigenic E. coli in food matrices

et al. 2022

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Enterohemorrhagic Escherichia coli (EHEC) O157:H7 and Enterotoxigenic E. coli (ETEC) are important foodborne pathogens, causing serious food poisoning outbreaks worldwide. Bacteriophages, as novel antibacterial agents, have been increasingly exploited to control foodborne pathogens. In this study, a novel broad-host range lytic phage vB_EcoM_SQ17 (SQ17), was isolated, characterized, and evaluated for its potential to control bacterial counts in vitro and in three different food matrices (milk, raw beef, and fresh lettuce). Phage SQ17 was capable of infecting EHEC O157:H7, ETEC, and other E. coli strains. Morphology, one-step growth, and stability assay showed that phage SQ17 belongs to the Caudovirales order, Myoviridae family, and Mosigvirus genus. It has a short latent period of 10 min, a burst size of 71 PFU/infected cell, high stability between pH 4 to 12 as well as thermostability between 30°C and 60°C for 60 min. Genome sequencing analysis revealed that the genome of SQ17 does not contain any genes associated with antibiotic resistance, toxins, lysogeny, or virulence factors, indicating the potential safe application of phage SQ17 in the food industry. In Luria-Bertani (LB) medium, phage SQ17 significantly decreased the viable counts of EHEC O157:H7 by more than 2.40 log CFU/ml (p < 0.05) after 6 h of incubation at 37°C. Phage SQ17 showed great potential to be applied for biocontrol of EHEC O157:H7 in milk and raw beef. In fresh lettuce, treatment with SQ17 also resulted in significant reduction of viable cell counts of EHEC O157:H7 and ETEC at both 4°C and 25°C. Our results demonstrate that SQ17 is a good candidate for application as an EHEC O157:H7 and ETEC biocontrol agent in the processing stages of food production and food preservation.

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A novel vibriophage vB_VhaS_PcB-1G capable of inhibiting virulent Vibrio harveyi pathogen

Cited by 17 publications

References 52 publications

Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi

Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi

Characterization and Genomic Analysis of a Bacteriophage with Potential in Lysing Vibrio alginolyticus

Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of Enterohemorrhagic Escherichia coli O157:H7 and Enterotoxigenic E. coli in food matrices

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