A novel visual test for hydrogen sulfide on the tongue dorsum

Kanehira, Takashi; Hongo, Hitomi; Takehara, Junji; Asano, Kozo; Osada, Kensuke; Izumi, Hiroshi; Fujii, Yoshihiro; Sakamoto, Wataru

doi:10.4236/ojst.2012.24054

Cited by 4 publications

(4 citation statements)

References 34 publications

(57 reference statements)

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“…Our results are comparable to previously reported ones, however slightly higher [ 53 , 54 , 55 ]. Generally, the analysis of H 2 S is a tricky procedure because of the instability of H 2 S, its high volatility and rapid oxidation.…”

Section: Resultssupporting

confidence: 91%

“…In our study, the collected saliva samples were tested instantly, whereas in other studies the samples were subjected to lengthy processing or storage. For example, in studies by Kaneshiro et al and Ritz et al saliva was collected by holding a cotton swab in the mouth for a few minutes [ 53 , 54 ]. Other methods require a chemical treatment with strong acid or base before analysis of H 2 S [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

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Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe

et al. 2018

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Ample evidence suggests that H2S is an important biological mediator, produced by endogenous enzymes and microbiota. So far, several techniques including colorimetric methods, electrochemical analysis and sulfide precipitation have been developed for H2S detection. These methods provide sensitive detection, however, they are destructive for tissues and require tedious sequences of preparation steps for the analyzed samples. Here, we report synthesis of a new fluorescent probe for H2S detection, 4-methyl-2-oxo-2H-chromen-7-yl 5-azidopentanoate (1). The design of 1 is based on combination of two strategies for H2S detection, i.e., reduction of an azido group to an amine in the presence of H2S and intramolecular lactamization. Finally, we measured salivary H2S concentration in healthy, 18–40-year-old volunteers immediately after obtaining specimens. The newly developed self-immolative coumarin-based fluorescence probe (C15H15N3O4) showed high sensitivity to H2S detection in both sodium phosphate buffer at physiological pH and in saliva. Salivary H2S concentration in healthy volunteers was within a range of 1.641–7.124 μM.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe

et al. 2018

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“…Furthermore, with respect to environmental studies, the assay is sensitive to metastable forms of iron sulfide deposits [such as amorphous FeS nanoparticles, Mackinawite (FeS), Greigite (Fe 3 S 4 ), or nanoparticle of pyrite (FeS 2 )] that might be present on cell surfaces or attached to biofilms/extracellular polymeric substance (EPS) matrix ( Morse and Rickard, 2004 ; Rickard and Morse, 2005 ). In human microbiology, the assay has also been adapted for sulfate reduction occurring in the oral cavity responsible for oral malodor ( Kanehira et al, 2012 ).…”

Section: Electron Acceptor Consumption Ratementioning

confidence: 99%

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

et al. 2020

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Viability and metabolic assays are commonly used as proxies to assess the overall metabolism of microorganisms. The variety of these assays combined with little information provided by some assay kits or online protocols often leads to mistakes or poor interpretation of the results. In addition, the use of some of these assays is restricted to simple systems (mostly pure cultures), and care must be taken in their application to environmental samples. In this review, the necessary data are compiled to understand the reactions or measurements performed in many of the assays commonly used in various aspects of microbiology. Also, their relationships to each other, as metabolism links many of these assays, resulting in correlations between measured values and parameters, are discussed. Finally, the limitations of these assays are discussed.

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“…A significant association was also noted between self-perceptions of oral malodor and frequent tongue coating. Tongue coating has been reported to be one of the typical factors of oral malodor [ 19 , 20 ]. Amou et al [ 21 ] visually evaluated the accumulation of tongue coating using Kojima's 5-step scoring criteria [ 22 ] and observed its correlations with the organoleptic score and CH 3 SH level.…”

Section: Discussionmentioning

confidence: 99%

Correlations between Perceived Oral Malodor Levels and Self-Reported Oral Complaints

Kameyama

Ishii

Tomita

et al. 2015

International Journal of Dentistry

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Objectives. Even though objective data indicating the absence of oral malodor are presented to patients, they may be skeptical about the results, possibly due to the presence of some discomfort in the oral cavity. The objective of this study was to investigate whether there is an association among self-perceptions of oral malodor, oral complaints, and the actual oral malodor test result. Materials and Methods. Questions concerning self-perceptions of oral malodor and subjective intraoral symptoms were extracted from a questionnaire on oral malodor completed by 363 subjects who visited the clinic for oral malodor of Tokyo Dental College Chiba Hospital and gave consent to this study. In addition, the association of self-perception of oral malodor with values obtained after organoleptic and OralChroma measurement was analyzed. Results. No correlation between 195 subjects (54%) who were judged “with oral malodor” (organoleptic score of ≥1) and 294 subjects (81.6%) who had a self-perceptions of oral malodor was observed. Self-perception of oral malodor was significantly correlated with tongue coating (p = 0.002) and a strange intraoral taste (p = 0.016). Conclusions. Subjects with a self-perception of oral malodor were not necessarily consistent with those actually having an oral malodor. In addition, it was suggested that patients became aware of oral malodor when they felt oral complaints.

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A novel visual test for hydrogen sulfide on the tongue dorsum

Cited by 4 publications

References 34 publications

Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe

Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

Correlations between Perceived Oral Malodor Levels and Self-Reported Oral Complaints

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