A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice blast disease resistance

Li, Hui; Zhou, Shijiao; Zhao, Wensheng; Su, Shiming; Peng, You-Liang

doi:10.1007/s11103-008-9430-5

Cited by 177 publications

(114 citation statements)

References 42 publications

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“…Such an expression pattern was also observed for genes encoding proteins containing protein kinase (Fig. 2b), leucine-rich, NB-ARC and EF-hand domains, which might function in signal transduction for defence systems (Kandoth et al, 2007;Lee & Rudd, 2002;Li et al, 2009;Syam Prakash & Jayabaskaran, 2006;Tameling & Baulcombe, 2007). These observations indicate that host defence systems associated with genes for transcription factors such as WRKY (OsWRKY45; Shimono et al, 2007) and NAC (ONAC28; Fang et al, 2008), and signal transduction-related genes such as those for protein kinase (CDPK) and receptor protein (NBS-LRR), were not activated at the early phase of RSV infection, but some steps of these systems were induced at the late phase, when the infected plants showed obvious symptoms.…”

Section: Modification Of Host Defence Systems By Rsv Infectionmentioning

confidence: 63%

Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection

Satoh

Kondoh

Sasaya³

et al. 2009

Journal of General Virology

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Rice stripe disease, caused by rice stripe virus (RSV), is one of the major virus diseases in east Asia. Rice plants infected with RSV usually show symptoms such as chlorosis, weakness, necrosis in newly emerged leaves and stunting. To reveal rice cellular systems influenced by RSV infection, temporal changes in the transcriptome of RSV-infected plants were monitored by a customized rice oligoarray system. The transcriptome changes in RSV-infected plants indicated that protein-synthesis machineries and energy production in the mitochondrion were activated by RSV infection, whereas energy production in the chloroplast and synthesis of cell-structure components were suppressed. The transcription of genes related to host-defence systems under hormone signals and those for gene silencing were not activated at the early infection phase. Together with concurrent observation of virus concentration and symptom development, such transcriptome changes in RSV-infected plants suggest that different sets of various host genes are regulated depending on the development of disease symptoms and the accumulation of RSV. INTRODUCTIONRice stripe disease is the most severe virus disease of rice in east Asia. Typical symptoms are chlorosis and weakness on newly emerged leaves. The plant becomes considerably stunted when affected at the early growth stages (Ou, 1972). Rice stripe disease also causes necrosis of newly emerged leaves (Takahashi et al., 1991). The causal virus is Rice stripe virus (RSV), which belongs to the genus Tenuivirus (Falk & Tsai, 1998). RSV is transmitted by small brown planthopper (SBPH; Laodelphax striatellus), Terthron albovittatum, Unkanodes sapporonus and Unkanodes albifascia (Falk & Tsai, 1998;Ou, 1972). RSV has a thin, filamentous shape and no envelope. The genome consists of four single-stranded RNA segments; RNA1 is negative-sense and RNAs 2-4 are ambisense. Viral mRNAs transcribed from viral RNA or viral cRNA by RNA-dependent RNA polymerase are released to the cytoplasm. Subsequently, a 59-capped short ribonucleotide leader cleaved from the host mRNA is added to the viral mRNAs by cap-snatching. The 59-capped RSV RNA is transcribed efficiently in host cells (Falk & Tsai, 1998;Shimizu et al., 1996). Genes encoding a gene-silencing suppressor and movement proteins were also identified in the RSV genome (Lu et al., 2009;Xiong et al., 2008Xiong et al., , 2009. Although extensive functional analysis of the RSV genome has been conducted, there have been no reports on the interaction between RSV and rice plants, which may clarify the mechanisms behind the appearance of disease symptoms.Rice is a model cereal plant, for which many genomic and transcriptome resources and tools are already available (Liang et al., 2008;Ouyang et al., 2007; Rice Annotation Project, 2008; Rice Full-length cDNA Consortium, 2003). The elucidation of genome sequences and structures in diverse organisms has led to the development of various high-throughput genome and transcriptome analytical tools. Numerous transcriptome profiles in...

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Section: Modification Of Host Defence Systems By Rsv Infectionmentioning

confidence: 63%

Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection

Satoh

Kondoh

Sasaya³

et al. 2009

Journal of General Virology

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“…Wall-associated kinases have been found to play a role in disease resistance (30,31), phosphorus-starvation tolerance (32), and developmental processes such as root growth (33) and gametophyte development (34). A recent study reported that the maize quantitative head smut resistance gene qHSR1 also encodes a wall-associated kinase.…”

Section: Resultsmentioning

confidence: 99%

The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

Hurni

Scheuermann

Krattinger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

277

221

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Northern corn leaf blight (NCLB) caused by the hemibiotrophic fungus Exserohilum turcicum is an important foliar disease of maize that is mainly controlled by growing resistant maize cultivars. The Htn1 locus confers quantitative and partial NCLB resistance by delaying the onset of lesion formation. Htn1 represents an important source of genetic resistance that was originally introduced from a Mexican landrace into modern maize breeding lines in the 1970s. Using a high-resolution map-based cloning approach, we delimited Htn1 to a 131.7-kb physical interval on chromosome 8 that contained three candidate genes encoding two wall-associated receptor-like kinases (ZmWAK-RLK1 and ZmWAK-RLK2) and one wall-associated receptor-like protein (ZmWAK-RLP1). TILLING (targeting induced local lesions in genomes) mutants in ZmWAK-RLK1 were more susceptible to NCLB than wild-type plants, both in greenhouse experiments and in the field. ZmWAK-RLK1 contains a nonarginine-aspartate (non-RD) kinase domain, typically found in plant innate immune receptors. Sequence comparison showed that the extracellular domain of ZmWAK-RLK1 is highly diverse between different maize genotypes. Furthermore, an alternative splice variant resulting in a truncated protein was present at higher frequency in the susceptible parents of the mapping populations compared with in the resistant parents. Hence, the quantitative Htn1 disease resistance in maize is encoded by an unusual innate immune receptor with an extracellular wall-associated kinase domain. These results further highlight the importance of this protein family in resistance to adapted pathogens.wall-associated receptor-like kinase | quantitative disease resistance | pattern recognition receptor | Exserohilum turcicum | maize

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“…Through an extracellular domain, wall-associated kinases physically link the plasma membrane to the cell wall matrix. They also have the potential to directly signal cellular events through their cytoplasmic kinase domain, which is one of the most likely candidates participating in cell wall-cytoplasm signaling in plant defense reactions [26]. All of these results suggest that the higher activity of defensive proteins and membrane fluidity under control conditions might be involved in CT in K354.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide gene expression profiling of introgressed indica rice alleles associated with seedling cold tolerance improvement in a japonica rice background

Zhang¹,

Huang²,

Wang³

et al. 2012

BMC Genomics

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BackgroundRice in tropical and sub-tropical areas is often subjected to cold stress at the seedling stage, resulting in poor growth and yield loss. Although japonica rice is generally more cold tolerant (CT) than indica rice, there are several favorable alleles for CT exist in indica that can be used to enhance CT in rice with a japonica background. Genome-wide gene expression profiling is an efficient way to decipher the molecular genetic mechanisms of CT enhancement and to provide valuable information for CT improvement in rice molecular breeding. In this study, the transcriptome of the CT introgression line (IL) K354 and its recurrent parent C418 under cold stress were comparatively analyzed to explore the possible CT enhancement mechanisms of K354.ResultsA total of 3184 differentially expressed genes (DEGs), including 195 transcription factors, were identified in both lines under cold stress. About half of these DEGs were commonly regulated and involved in major cold responsive pathways associated with OsDREB1 and OsMyb4 regulons. K354-specific cold-induced genes were functionally related to stimulus response, cellular cell wall organization, and microtubule-based movement processes that may contribute to increase CT. A set of genes encoding membrane fluidity and defensive proteins were highly enriched only in K354, suggesting that they contribute to the inherent CT of K354. Candidate gene prediction based on introgressed regions in K354 revealed genotype-dependent CT enhancement mechanisms, associated with Sir2, OsFAD7, OsWAK112d, and programmed cell death (PCD) related genes, present in CT IL K354 but absent in its recurrent parent C418. In K354, a number of DEGs were co-localized onto introgressed segments associated with CT QTLs, providing a basis for gene cloning and elucidation of molecular mechanisms responsible for CT in rice.ConclusionsGenome-wide gene expression analysis revealed that genotype-specific cold induced genes and genes with higher basal expression in the CT genotype contribute jointly to CT improvement. The molecular genetic pathways of cold stress tolerance uncovered in this study, as well as the DEGs co-localized with CT-related QTLs, will serve as useful resources for further functional dissection of the molecular mechanisms of cold stress response in rice.

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A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice blast disease resistance

Cited by 177 publications

References 42 publications

Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection

Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection

The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

Genome-wide gene expression profiling of introgressed indica rice alleles associated with seedling cold tolerance improvement in a japonica rice background

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