A novel xenograft model with intrinsic vascularisation for growing undifferentiated pleomorphic sarcoma NOS in mice

Tilkorn, Daniel J.; Daigeler, Adrien; Hauser, Joerg; Ring, Andrej; Stricker, Ingo; Schmitz, Inge; Steinstraesser, Lars; Steinau, Hans-Ulrich; Al-Benna, Sammy

doi:10.1007/s00432-012-1163-9

Cited by 5 publications

(7 citation statements)

References 27 publications

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“…Undifferentiated pleomorphic sarcomas account for 5% of adult soft tissuesarcomas4. They predominantly present in the 5th to 7th decades of life and occur most frequently in the lower extremities3. Although undifferentiated pleomorphic sarcoma has high incidences of local recurrence and metastasis, to our knowledge, only 10 cases of cutaneous metastasis have been reported.…”

Section: Introductionmentioning

confidence: 99%

“…Undifferentiated pleomorphic sarcomas are a group of pleomorphic high-grade sarcomas that cannot be classified into any other subtype1,2. Undifferentiated pleomorphic sarcomas, known as malignant fibrous histiocytoma, is a malignant neoplasm that arises both in soft tissue and bones1,3. Histopathologically, it is described as an undifferentiated pleomorphic sarcoma characterized by atypical pleomorphic spindle tumor cells with a storiform growth pattern3.…”

Section: Introductionmentioning

confidence: 99%

“…Undifferentiated pleomorphic sarcomas, known as malignant fibrous histiocytoma, is a malignant neoplasm that arises both in soft tissue and bones1,3. Histopathologically, it is described as an undifferentiated pleomorphic sarcoma characterized by atypical pleomorphic spindle tumor cells with a storiform growth pattern3. Undifferentiated pleomorphic sarcomas account for 5% of adult soft tissuesarcomas4.…”

Section: Introductionmentioning

confidence: 99%

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Cutaneous Metastatic Undifferentiated Pleomorphic Sarcoma from a Mediastinal Sarcoma

2015

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Undifferentiated pleomorphic sarcoma, known as malignant fibrous histiocytoma, is a malignant neoplasm that arises in both soft tissue and bones. In 2002, the World Health Organization declassified malignant fibrous histocytoma as a formal diagnostic entity and renamed it 'undifferentiated pleomorphic sarcoma not otherwise specified.' It most commonly occurs in the lower extremities and rarely metastasizes cutaneously. We report a case of cutaneous metastatic undifferentiated pleomorphic sarcoma of the buttocks occurring in a 73-year-old man diagnosed with mediastinal sarcoma 4 years previously. He first noticed the mass approximately 2 months previously. Histological findings with immunomarkers led to a final diagnosis of cutaneous metastatic sarcoma from mediastinal undifferentiated pleomorphic sarcoma.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cutaneous Metastatic Undifferentiated Pleomorphic Sarcoma from a Mediastinal Sarcoma

2015

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“…In contrast to other approaches using solid tumor tissue or silicon chambers to place tumor fragments around the superficial epigastric vessels (26,27), the present study generated stable oligoclonal cell cultures from freshly isolated tumor tissue of two patients with undifferentiated pleomorphic sarcoma, which were similar to the oligoclonal variety exhibited by the original tumors. Additionally, these cultures were subcutaneously injected into immunodeficient mice to establish xenograft animal models.…”

Section: Discussionmentioning

confidence: 97%

“…However, investigations comparing the effect of therapeutical approaches in vivo and in vitro have not been performed thus far. In other studies using xenotransplantation models, original human tumor tissue was transplanted into immunodeficient mice (25)(26)(27). These animal models appear to be more similar to the tumors observed in humans, but hypoxia following tissue transplantation remains a problem (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Xenograft models for undifferentiated pleomorphic sarcoma not otherwise specified are essential for preclinical testing of therapeutic agents

et al. 2016

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Abstract. Undifferentiated pleomorphic sarcoma not otherwise specified belongs to the heterogeneous group of soft tissue tumors. It is preferentially located in the upper and lower extremities of the body, and surgical resection remains the only curative treatment. Preclinical animal models are crucial to improve the development of novel chemotherapeutic agents for the treatment of undifferentiated pleomorphic sarcoma. However, this approach has been hampered by the lack of reproducible animal models. The present study established two xenograft animal models generated from stable non-clonal cell cultures, and investigated the difference in chemotherapeutic effects on tumor growth between undifferentiated pleomorphic sarcoma in vivo and in vitro. The cell cultures were generated from freshly isolated tumor tissues of two patients with undifferentiated pleomorphic sarcoma. For the in vivo analysis, these cells were injected subcutaneously into immunodeficient mice. The mice were monitored for tumor appearance and treated with the most common or innovative chemotherapeutic agents available to date. Furthermore, the same drugs were administered to in vitro cell cultures. The most effective tumor growth inhibition in vitro was observed with doxorubicin and the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA), also known as vorinostat. In the in vivo xenograft mouse model, the combination of doxorubicin and the tyrosine kinase inhibitor pazopanib induced a significant tumor reduction. By contrast, treatment with vorinostat did not reduce the tumor growth. Taken together, the results obtained from drug testing in vitro differed significantly from the in vivo results. Therefore, the novel and reproducible xenograft animal model established in the present study demonstrated that in vivo models are required to test potential chemotherapeutic agents for the treatment of undifferentiated pleomorphic sarcoma prior to clinical use, since animal models are more similar to humans, compared with in vitro cell cultures.

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