A novelty strategy for the fast analysis of sulfonamide antibiotics in fish tissue using magnetic separation with high‐performance liquid chromatography–tandem mass spectrometry

Abstract

A simple, fast and low-cost extraction method with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) determination was developed on sulfonamide antibiotics (SAs) in fish tissue. Magnetic separation was first introduced into the rapid sample preparation procedure combined with acetonitrile extraction for the analysis of SAs. Partitioning was rapidly achieved between acetonitrile solution and solid matrix by applying an external magnetic field. Acetonitrile solution was collected and concentrated under a nitrogen stream. The residue was redissolved with 1‰ formic acid aqueous solution and defatted with n-hexane before analysis. The recoveries of SAs were in the range of 74.87-104.74%, with relative standard deviations <13%. The limits of quantification and the limits of detection for SAs ranged from 5.0 to 25.0 μg (kg-1) and from 2.5 to 10.0 μg (kg-1) , respectively. The presented extraction method proved to be a rapid method which only took 20 min for one sample preparation procedure. Copyright © 2016 John Wiley & Sons, Ltd.

Food Sample Preparation for the Determination of Sulfonamides by High-Performance Liquid Chromatography: State-of-the-Art

“…In the case of animal tissue samples, SLE [31][32][33], ASE [34][35][36], QuEChERS extraction [37,38], SALLE [40,41], and SPE [42] were the most reported methodologies for the extraction of SAs, along with other antibiotic or veterinary drugs, followed by reports of USE [36], DSPE [45], and SBSE [48]. Reports of on-line techniques include a fully automated on-line QuEChERS extraction protocol [39] and an on-line SPME protocol that utilized molecularly imprinted monolithic capillary columns [47].…”

“…The aqueous phase was collected and passed through a 0.22 µm syringe filter and analyzed with HPLC-MS/MS. The authors emphasized the good recoveries and the reduced time requirements of the developed sample preparation protocol [40]. In the second report, the samples were treated with ethyl acetate and concentrated under vacuum.…”

Sample preparation in foodomic analyses

“…As presented in Fig. B, for extraction and fractionation of solid (and sometimes also semisolid and very viscous food samples), the following are used: (i) matrix solid‐phase dispersion , (ii) pressurized liquid extraction , (iii) super‐heated water extraction (SHWE ), (iv) SFE , (v) microwave and ultrasound‐assisted SPE , and (vi) magnetic SPE . The MSPD involves the process of complete sample disruption by mechanical forces and the interactions of the sample matrix with the solid support surface.…”

Employment of modified Fe3O4 nanoparticles using thermo‐sensitive polymer for extraction and pre‐concentration of cefexime in biological samples

“…Among them, magnetic nanoparticles have attracted extensive attention owing to their wide range of applications in magnetic resonance imaging (Seo et al, ), drug delivery (Zhang et al, ) and hyperthermia treatment (Mornet, Vasseur, Grasset, & Duguet, ). Also, these nanoparticles (NPs) can be used for analytical applications (Fan, Wang, & Wei, ; Hussain, Alajmi, & Ali, ; Li, Liu, Zhang, Liu, & Wu, ; Ni, Chen, Dong, Tian, & Bai, ; Zhao, Liao, & Yang, ). To perform sample treatment, Fe 3 O 4 NPs play a vital role in analytical nanotechnology.…”