A nuclear magnetic resonance study of the ribotrinucleoside dlphophate UpUpC

Abstract

500 MHz 1H, 67.89 MHz 13C and 80.97 MHz 31P-NMR studies are reported on the ribotrinucleoside diphosphate UpUpC, the triplet codon corresponding to the amino acid phenylalanine. Complete spectral assignments are given and conformational parameters for the backbone and the furanose rings are determined. All three nucleotide units show a near-balance for the N/S equilibrium with a slight preference for the N-type ribose (approximately 60%). The backbone conformation around the C3'-03' bonds show a preference for the trans domain, while the orientation around the C5'-05' bonds is predominantly trans.

The application of double-quantum and spin echo nuclear magnetic resonance spectroscopy to the assignment of 1H chemical shifts of RNA oligomers

“…The remaining chemical shift assignments are summarized in Table 1. This analysis was in agreement with recently published data on UpUpC, obtained at 500 MHz (21).…”