A nucleotide sequence variation detection system for the core region of hepatitis C virus-1b

Okamoto, K.; Akuta, Norio; Kumada, Hiromitsu; Kobayashi, Mariko; Matsuo, Yuki; Tazawa, Hiromitsu

doi:10.1016/j.jviromet.2006.11.020

Cited by 14 publications

(11 citation statements)

References 6 publications

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“…The HCV core sequence can be easily amplified using PCR because of its conservative nature and analysis of only two amino acid positions is timesaving; therefore, this method might be feasible for identifying predictive markers for HCC. A specific PCR method for detecting these mutations was reported [36]. Furthermore, we developed a rapid and sensitive real-time PCR method for quantitatively detecting these mutations [37].…”

Section: Discussionmentioning

confidence: 99%

Association between mutations in the core region of hepatitis C virus genotype 1 and hepatocellular carcinoma development

Nakamoto

Imazeki

Fukai

et al. 2010

Journal of Hepatology

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Section: Discussionmentioning

confidence: 99%

Association between mutations in the core region of hepatitis C virus genotype 1 and hepatocellular carcinoma development

Nakamoto

Imazeki

Fukai

et al. 2010

Journal of Hepatology

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“…(12) All patients have completed 24 weeks after cessation of treatment, and SVR could be evaluated. (13) Each signed a consent form of the study protocol that had been approved by the Human Ethics Review Committee of Toranomon Hospital.…”

Section: Study Populationmentioning

confidence: 99%

Predictive factors of early and sustained responses to peginterferon plus ribavirin combination therapy in Japanese patients infected with hepatitis C virus genotype 1b: Amino acid substitutions in the core region and low-density lipoprotein cholesterol levels

Akuta

Suzuki

Kawamura

et al. 2007

Journal of Hepatology

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296

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“…Amino acid (aa) at position 70 of Arg or Gln and aa91 of Leu or Met were determined by PCR with primers specific for each of them [Okamoto et al, 2007]. It is highly reproducible, and has a sensitivity of 94.4% in the determination of aa70 or aa91 in samples with HCV RNA titers >10 KIU/ml.…”

Section: Determination Of Amino-acid Substitutions In the Core Proteinmentioning

confidence: 99%

Influence of amino‐acid polymorphism in the core protein on progression of liver disease in patients infected with hepatitis C virus genotype 1b

Kobayashi

Akuta

Suzuki

et al. 2009

Journal of Medical Virology

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The substitution of amino acid (aa) 70 of arginine for glutamine and/or that of aa91 of leucine for methionine in the core protein in patients infected with hepatitis C virus (HCV) genotype 1b is associated with a poor response to pegylated interferon and ribavirin. Factors influencing these substitutions were sought in 1,097 patients infected with HCV-1b who had not received antiviral treatment. HCV variants with Arg70 and Leu91 (wild-type) decreased, while those with Gln70 and/or Met91 (mutant types) increased with age (P < 0.001). Of the 1,097 patients, 464 (42.3%) were infected with the Gln70 variant and the remaining 633 patients with the Arg70 variant. The proportion of patients with the Gln70 variant increased with the severity of liver disease (P < 0.001), elevated gamma-glutamyl transpeptidase (gamma-GTP) levels (P < 0.001) and a decrease in platelet count (P = 0.008). In univariate analysis patients with hepatocellular carcinoma, elevated aspartate aminotransferase (AST > or = 58 IU/L) and gamma-GTP (> or =61 IU/L), and decreased albumin levels (<3.9 g/dl) were more frequent in the patients with the Gln70 variant than the Arg70 variant (P = 0.003, 0.005, <0.001, and 0.031, respectively). In multivariate analysis HCC (odds ratio 1.829 [95% confidence interval 1.147-2.917]) and gamma-GTP > or =61 IU/L (1.647 [1.268-2.139]) increased the risk for the Gln70 variant. In conclusion, the substitution of amino aa70 of Arg for Gln in patients infected with HCV-1b increases with age, and it is associated with severe liver disease accompanied by elevated AST and gamma-GTP levels, as well as the development of hepatocellular carcinoma.

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A nucleotide sequence variation detection system for the core region of hepatitis C virus-1b

Cited by 14 publications

References 6 publications

Association between mutations in the core region of hepatitis C virus genotype 1 and hepatocellular carcinoma development

Association between mutations in the core region of hepatitis C virus genotype 1 and hepatocellular carcinoma development

Predictive factors of early and sustained responses to peginterferon plus ribavirin combination therapy in Japanese patients infected with hepatitis C virus genotype 1b: Amino acid substitutions in the core region and low-density lipoprotein cholesterol levels

Influence of amino‐acid polymorphism in the core protein on progression of liver disease in patients infected with hepatitis C virus genotype 1b

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