A one health approach to vaccines against Toxoplasma gondii

Innes, Elisabeth A.; Hamilton, C. M.; Garcia, João Luís; Chryssafidis, Andréas Lazaros; Smith, David W.

doi:10.1016/j.fawpar.2019.e00053

Cited by 65 publications

(55 citation statements)

References 160 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Intranasal (IN) immunization is known to elicit mucosa immunity as well as systemic immunity [ 38 ]. Mucosal immunity induction is particularly important since T. gondii transmission mainly occurs through oral ingestion of the pathogen [ 39 , 40 ]. The CpG-ODN adjuvant has been proved to be a promising mucosal adjuvant [ 41 , 42 ] and induces better protective mucosal immunity after IN immunization [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of CpG-ODN-Adjuvanted Toxoplasma gondii Virus-Like Particle Vaccine upon One, Two, and Three Immunizations

et al. 2020

View full text Add to dashboard Cite

Successful vaccines against specific pathogens often require multiple immunizations and adjuvant usage. Yet, assessing the protective efficacy of different immunization regimens with adjuvanted Toxoplasma gondii vaccines remains elusive. In this study, we investigated the vaccine efficacy induced by CpG-ODN-adjuvanted T. gondii virus-like particles (VLPs) after challenge infection with T. gondii (ME49) in mice (BALB/c) upon one, two, and three immunizations. Immunization with adjuvanted T. gondii VLPs induced higher levels of T. gondii-specific IgG and/or IgA antibody responses, germinal center (GC) B cells, total B cells, and CD4+ and CD8+ T cells compared with unadjuvanted VLPs. Increasing the number of immunizations was strongly correlated with enhanced protective immunity against T. gondii in mice, with the highest protection being demonstrated in mice thrice-immunized with either adjuvanted T. gondii VLPs or VLPs alone. Notably, lesser bodyweight reductions and cerebral cyst counts were observed in mice receiving multiple immunizations with the adjuvanted VLPs, thereby confirming the effectiveness of adjuvanted boost immunizations. These results demonstrated that multiple immunizations with T. gondii VLPs is an effective approach, and the CpG-ODN can be developed as an effective adjuvant for T. gondii VLP vaccines.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of CpG-ODN-Adjuvanted Toxoplasma gondii Virus-Like Particle Vaccine upon One, Two, and Three Immunizations

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The one health approach is a transdisciplinary approach taking into account the infection and its consequence on the animal and human health and also, on the environment at the local and global levels. The one health response to control toxoplasmosis using vaccination will aim to prevent congenital disease in both humans and target animals, to produce safe meat for human consumption and to reduce environmental contamination with oocysts (32,33).…”

Section: Introductionmentioning

confidence: 99%

Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep

et al. 2020

View full text Add to dashboard Cite

Toxoplasma gondii is a parasitic protozoan of worldwide distribution, able to infect all warm-blooded animals, but particularly sheep. Primary infection in pregnant sheep leads to millions of abortions and significant economic losses for the livestock industry. Moreover, infected animals constitute the main parasitic reservoir for humans. Therefore, the development of a One-health vaccine seems the best prevention strategy. Following earlier work, a vaccine constituted of total extract of Toxoplasma gondii proteins (TE) associated with maltodextrin nanoparticles (DGNP) was developed in rodents. In this study we evaluated the ability of this vaccine candidate to protect against latent and congenital toxoplasmosis in sheep. After two immunizations by either intranasal or intradermal route, DGNP/TE vaccine generated specific Th1-cellular immune response, mediated by APC-secretion of IFN-γ and IL-12. Secretion of IL-10 appeared to regulate this Th1 response for intradermally vaccinated sheep but was absent in intranasallyvaccinated animals. Finally, protection against latent toxoplasmosis and transplacental transmission were explored. Intranasal vaccination led to a marked decrease of brain cysts compared with the non-vaccinated group. This DGNP/TE vaccine administered intranasally conferred a high level of protection against latent toxoplasmosis and its transplacental transmission in sheep, highlighting the potential for development of such a vaccine for studies in other species.

show abstract

“…Additionally, spiramycine is used in CT to treat infection during pregnancy, but its administration is still controversial, since multicenter studies conducted in Europe have shown conflicting results about its efficacy (Sander et al, 2018 ). Although prevention of the severe consequences of CT in pregnant women would be a major target for a human vaccine, there has been scarce progress on this issue, a possible reason may be the fact that CT is generally not considered a priority for public health (Innes et al, 2019 ). There are also scientific obstacles, including that the best protective results of vaccines against T. gondii have been observed with live vaccine preparations, but there are safety and regulatory issues that may impede their use in humans (Innes et al, 2011 ).…”

Section: Veterinary Vaccine Development Against Fbds Caused By Parasimentioning

confidence: 99%

“…Killed or subunit vaccines represent a more safer approach, but there challenges to overcome, including that they are generally associated with a poor cellular immune response, one of the most important features to get a proper protection against T. gondii infections (Sander et al, 2018 , 2019 ). Recent advances in understanding the key protective immune responses may help to determine predictive algorithms for protective epitopes in order to design better subunit vaccines (Innes et al, 2019 ). In addition, as CT is similar in women and sheep, an animal model based in sheep may be helpful in progressing a vaccine against human CT (Innes and Vermeulen, 2006 ).…”

Section: Veterinary Vaccine Development Against Fbds Caused By Parasimentioning

confidence: 99%

“…The authors demonstrated that administration of 500 μg of a cocktail DNA vaccine + pJV2004 and pJV2005 as adjuvants is able to elicit humoral and cellular immune responses against T. gondii in pigs and the protection achieved was around 70%. Similarly, efforts are being made to find a safer vaccine formulation for sheep, and this is clearly due to the significant economic and welfare impacts that CT has on the sheep livestock worldwide (Innes et al, 2019 ). In addition, this species may also be a more relevant animal model than the mouse to test potential human vaccines against CT, as the disease in pregnant sheep and pregnant women has similarities, as previously mentioned (Innes and Vermeulen, 2006 ).…”

Section: Veterinary Vaccine Development Against Fbds Caused By Parasimentioning

confidence: 99%

See 1 more Smart Citation

Use of Veterinary Vaccines for Livestock as a Strategy to Control Foodborne Parasitic Diseases

Sander

López

Morales

et al. 2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Foodborne diseases (FBDs) are a major concern worldwide since they are associated with high mortality and morbidity in the human population. Among the causative agents of FBDs, Taenia solium, Echinococcus granulosus, Toxoplasma gondii, Cryptosporidium spp., and Trichinella spiralis are listed in the top global risk ranking of foodborne parasites. One common feature between them is that they affect domestic livestock, encompassing an enormous risk to global food production and human health from farm to fork, infecting animals, and people either directly or indirectly. Several approaches have been employed to control FBDs caused by parasites, including veterinary vaccines for livestock. Veterinary vaccines against foodborne parasites not only improve the animal health by controlling animal infections but also contribute to increase public health by controlling an important source of FBDs. In the present review, we discuss the advances in the development of veterinary vaccines for domestic livestock as a strategy to control foodborne parasitic diseases.

show abstract

A one health approach to vaccines against Toxoplasma gondii

Cited by 65 publications

References 160 publications

Evaluation of CpG-ODN-Adjuvanted Toxoplasma gondii Virus-Like Particle Vaccine upon One, Two, and Three Immunizations

Evaluation of CpG-ODN-Adjuvanted Toxoplasma gondii Virus-Like Particle Vaccine upon One, Two, and Three Immunizations

Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep

Use of Veterinary Vaccines for Livestock as a Strategy to Control Foodborne Parasitic Diseases

Contact Info

Product

Resources

About