A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva

Oura, C.A.L.; Odongo, David; Lubega, George W.; Spooner, P.R.; Tait, Andrew; Bishop, Richard P.

doi:10.1016/s0020-7519(03)00280-7

Cited by 78 publications

(102 citation statements)

References 40 publications

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“…This contrasts with earlier observations in a naïve animal infected with St72, where approximately 75% of clones derived from lymph node aspirates bore the 72-01 genotype (13). Eleven genotypes were observed among 62 clones derived from the day 9 aspirate, and one, designated 211-A3, differed from the 72-01 genotype at only one locus (ms010) (24). The day 14 aspirate yielded 95 clones, among which 10 distinct genotypes were observed, while 3 genotypes were detected among 5 clones isolated from the day 16 aspirate.…”

Section: Resultscontrasting

confidence: 95%

Influence of Host Immunity on Parasite Diversity inTheileria parva

et al. 2007

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We examined the influence of host immunity on the genotypic diversity of the intracellular transforming cattle parasite Theileria parva. By tracking the emergence of discrete parasite genotypes in an animal challenged with a bulk stabilate following immunization with its major component clone, we observed a profound modulation of genotypic frequencies in the breakthrough schizont population. In particular, no incidences of the immunizing clone were observed and a progressive decline was apparent in the relatedness of breakthrough genotypes to it. These observations were reflected in the genotypic profile of transmissible parasite stages that emerged in the erythrocyte fraction of the animal and in parasite progeny generated by tick pickup. In a separate experiment, genotypic profiles of breakthrough parasite populations were observed to vary between unrelated immune animals selected on the basis of the major histocompatibility complex (MHC) class I phenotype, a known determinant of the specificity of the immune response. Furthermore, immunization and challenge of calves with molecularly distinct but cross-protective parasite populations revealed that infection results in transmissible erythrocyte forms in spite of a protective immune response. These observations suggest that immunity does not prevent transmission of challenge parasites and that its impact on the parasite at a population level is influenced by herd MHC diversity.

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Section: Resultscontrasting

confidence: 95%

Influence of Host Immunity on Parasite Diversity inTheileria parva

et al. 2007

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“…Using an expanded panel of satellite and other polymorphic markers based on those described by Oura et al (26), considerable genotypic variation was observed in the stock, despite only limited diversity at individual loci. Although only two or three alleles were observed at the majority of loci, 48 distinct locus combinations were observed in St72 of the stock, with one, 72-01, being highly dominant.…”

Section: Discussionmentioning

confidence: 99%

“…(i) Satellite markers. The satellite markers used here were those described by Oura et al (26) supplemented with an additional 13 polymorphic markers that had previously been excluded due to cross-reaction with other Theileria species. The additional markers were chosen on the basis of their location on the four chromosomes and are outlined in Table 2.…”

Section: Methodsmentioning

confidence: 99%

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Extensive Genotypic Diversity in a Recombining Population of the Apicomplexan ParasiteTheileria parva

et al. 2006

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We evaluated sexual recombination in the apicomplexan parasite Theileria parva using genome-wide marker analysis of haploid sporozoite populations obtained from infected Rhipicephalus appendiculatus ticks. Analysis of 231 parasite clones derived by in vitro infection of bovine lymphocytes revealed 48 distinct combinations of 64 polymorphic marker loci. One genotype accounted for more than 75% of the clones, and the population was highly inbred with respect to this. The occurrence of frequent recombination was evident from reassortment of contiguous markers in blocks, with some recombination occurring within blocks. Analysis of four polymorphic loci encoding antigens targeted by protective cytotoxic-T-lymphocyte responses confirmed that these loci reassort, both within and between chromosomes, suggesting that recombination may influence immune recognition. Marker analysis of a panel of 142 clones derived from the population after an additional passage through a calf and the same tick colony revealed 18 genotypes, with the original dominant genotype accounting for 75% of the population and a higher level of inbreeding with respect to it in the remaining clones. Selected marker analysis of genomic DNA from these stabilates and the two preceding generations of the isolate, each derived from distinct tick colonies, revealed shifts in population structure with each generation, suggesting that the tick vector may impose nonrandom selective pressure on the parasite.

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“…Theileria parva (15)(16)(17) ve T. annulata (18)'nın popülasyon yapıları ile ilgili polimorfik mini ve mikrosatellit marker kullanılarak yapı-lan çalışmalarda, bu parazitlerin popülasyonlarında genetik değişimin sıklıkla meydana geldiği belirlenmiştir. Deneysel olarak, T. parva'da seksüel rekombinasyonun varlığı moleküler düzeyde kanıtlanmıştır (5).…”

Section: Introductionunclassified

Selection of Genetic Markers to Determine Diversity in Theileria annulata Populations after Recombination

Bilgiç¹,

Ünlü²,

Aksulu³

et al. 2017

TurkiyeParazitolDerg

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ÖZAmaç: Theileria annulata popülasyonlarında rekombinasyon sonrası oluşacak genetik çeşitliliğin ve rekombinasyonun yoğun olduğu kromozomal bölgelerin tespit edilmesi amacıyla kullanılacak polimorfik mini ve mikrosatellite markerlerin belirlenmesidir. Yöntemler: Markerlerin belirlenmesi amacıyla Unipro UGENE programı kullanılmıştır. T. annulata'nın rastgele karıştırılmış genomik dizilimlere göre gerçek DNA diziliminde 10 kat fazla tandem tekrar (TR) belirleyen skor, eşik değer olarak belirlenmiştir. Tekrarlı motif benzerliği %96-100 arasında bulunan, minimum tekrar sayısı en az üç ve minimum tandem boyutu minisatellite bölgeler için en az üç nükleotid ve mikrosatellite bölgeler için en az altı nükleotid olan TR'ler suffix array algoritması kullanılarak tüm genom boyunca taranmıştır. Bulgular: Tarama sonrası 359 minisatellite ve 8973 mikrosatellite bölge belirlenmiştir. TR'ler tüm genom boyunca tek tek taranarak; T. annulata'nın kromozomları üzerindeki yerleşim yerlerine, motiflerin benzerliğine (>%96), tekrar sayıları ile tek bölgeyi temsil etmesine, bölgelerin uzunluklarına (<1500 bp) ve primer tasarlanmaya uygun korunmuş bölgeler içermesine bakılarak mini ve mikrosatellite bölgeler belirlenmiştir. Belirlenen bölgeleri çoğaltmada kullanılacak primerler, korunmuş bölgelerden tasarlanmış ve her bir primer, T. annulata'nın farklı izolatları kullanılarak değerlendirilmiştir. Sonuç: Bu çalışmada, T.annulata popülasyonlarındaki çeşitliliğinin belirlenmesi amacıyla daha önce belirlenen markerlere ilave olarak kullanılabile-cek, farklı kromozomlar üzerinde bulunan toplam 13 mini ve mikrosatellite marker seçilmiştir. Anahtar Kelimeler: Theileria annulata, rekombinasyon, satellit marker Geliş Tarihi: 17.06.2016Kabul Tarihi: 21.12.2016 ABSTRACT Objective: Selecting polymorphic mini-and microsatellite markers to determine genetic diversity and chromosomal regions exhibiting elevated rates of recombination in Theileria annulata populations after recombination. Methods: The Unipro UGENE software was used to select markers. A score at which 10 times more tandem repeats (TRs) were identified in the real DNA sequence than those in the scrambled sequences of T. annulata was used as the cutoff. TRs containing minimum three nucleotides in length for microsatellite and six nucleotides for minisatellite regions and having a repeat motif identity between 96%-100% with the unit size repeated minimum three times were screened through the whole genome using the suffix array algorithm.Results: A total of 359 minisatellites and 8973 microsatellites were identified. TRs were screened one by one through the whole genome; mini-and microsatellites representing a single region and having suitable regions for primer design were selected based on their localization on T. annulata chromosomes, their repeat motif identity (>96%), and their repeat length (<1500 bp). The primers used to amplify selected candidates were designed, and each primer was used to check 27 different isolates of T. annulata. Conclusion: In the present study, a total of 13 po...

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A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva

Cited by 78 publications

References 40 publications

Influence of Host Immunity on Parasite Diversity inTheileria parva

Influence of Host Immunity on Parasite Diversity inTheileria parva

Extensive Genotypic Diversity in a Recombining Population of the Apicomplexan ParasiteTheileria parva

Selection of Genetic Markers to Determine Diversity in Theileria annulata Populations after Recombination

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