A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

Abstract

An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free β-agonists compete for the binding sites. This enables rapid screening for the 7 β-agonists in a single run that takes about 8 min. Detection limits for the seven β-agonists are <50 pg g−1 of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were ±15%. The assay was applied to the analysis of both using spiked and unspiked pork for β-agonists, and the results compare well to those obtained by HPLC-MS. Graphical abstractSchematic presentation of an ultra sensitive fluorescent nanoparticle based paper based assay for rapid detection of multi β-agonists in pork tissue. Electronic supplementary materialThe online version of this article (10.1007/s00604-018-2730-9) contains supplementary material, which is available to authorized users.

A review on advances in methods for modification of paper supports for use in point-of-care testing

“…A wax-based technology was applied to modify hydrophobic patterns and hydrophobic barriers on paper using a wax printer [27] and a pen-writing approach [28]. Different nanomaterials, such as quantum dots (QDs) [29][30][31][32][33], gold nanoparticles (AuNPs) [24,[34][35][36][37][38][39][40][41][42][43][44][45][46], AuNFs MBA @Ag [47] Cu-Pd/rGO nanoparticles [48], upconverting fluorescent nanocrystals [49], metal-organic framework [50], fluorescent nanoparticles [51], gold nanocage [52], gold nanorods [53], multi-walled carbon nanotubes and graphene oxide [54] and polymer nanoparticles [55], have also been used as reading signal in paper-based POCT devices. The functional papers prepared by these advanced modification approaches significantly extend the application potential of paper as the substrate of POCT devices.…”

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

“…To further improve the sensitivity of LFIA, other labels such as fluorescent nanoparticles have been employed in LFIA fabrication. An ultrasensitive fluorescent LFIA has been prepared with a nonspecific monoclonal antibody [35]. This fluorescent LFIA can rapidly screen for seven β-agonists within 8 min with an LOD lower than 50 pg/g of pork.…”

Multiplex Lateral Flow Immunoassay: An Overview of Strategies Towards High-Throughput Point-of-Need Testing

“…In one sense, broad-specific recognition elements have been developed with the aim of detecting several compounds in a group or a class. As a matter of fact, class-selective antibodies have been prepared and used to measure several analytes (within a class of compounds) by means of LF devices [27][28][29]: Zhang et al developed a monoclonal antibody, which was able to recognize three major ochratoxins, and based on this mAb, they proposed a LFIA for the simultaneous detection of the three hazardous substances. [27].…”

“…Similarly, Xie et al generated a monocolonal antibody that equally recognizes avermectin and ivermectin and employed it for setting up a dual LFIA [28]. Notably, Wang R. et al reported a xLFIA for measuring up to 7 β-agonists in a single run by using a monoclonal that recognized clenbuterol and its analogues [29].…”