A Parallel-Plate Flow Chamber to Study Initial Cell Adhesion on a Nanofeatured Surface

Martines, Elena; Mcghee, K.; Wilkinson, C. D. W.; Curtis, Adam

doi:10.1109/tnb.2004.828268

Cited by 69 publications

(41 citation statements)

References 37 publications

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“…Mechanisms underpinning attachment to nanoscale topographies for mammalian cells is a greatly studied area which has continued to foster significant findings and major contributions to the wealth of knowledge on eukaryotic cell behavior [82][83][84][85]. However, as Hsu et al [48] have described, attachment phenomena for bacterial cells at the nanoscale is less understood, as relatively few reported works have explored the effects of nanoscale topography on bacterial attachment behavior and biofilm formation [23,49,54,86,87].…”

Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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Bacterial surface fouling is problematic for a wide range of applications and industries, including, but not limited to medical devices (implants, replacement joints, stents, pacemakers), municipal infrastructure (pipes, wastewater treatment), food production (food processing surfaces, processing equipment), and transportation (ship hulls, aircraft fuel tanks). One method to combat bacterial biofouling is to modify the topographical structure of the surface in question, thereby limiting the ability of individual cells to attach to the surface, colonize, and form biofilms. Multiple research groups have demonstrated that micro and nanoscale topographies significantly reduce bacterial biofouling, for both individual cells and bacterial biofilms. Antifouling strategies that utilize engineered topographical surface features with well-defined dimensions and shapes have demonstrated a greater degree of controllable inhibition over initial cell attachment, in comparison to undefined, texturized, or porous surfaces. This review article will explore the various approaches and techniques used by researches, including work from our own group, and the underlying physical properties of these highly structured, engineered micro/nanoscale topographies that significantly impact bacterial surface attachment.

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Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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“…Since osteoblast adhesion occurred preferentially at surface particle boundaries and more of them are present on the nanophase compared to conventional material, this may explain the observed increase in osteoblast adhesion [Webster and Ejiofor, 2004]. However, this phenomenon may be cell specifi c, since reduced fi broblast adhesion was observed on nanosized pits (PMMA, 35-120 nm in diameter) Martines et al, 2004]. We observed well-formed focal contacts by fl uorescence labeling for vinculin only at later time points (6 and 24 h), but not during the fi rst 90 min of cell adhesion.…”

Section: Surface Composition and Cell Adhesionmentioning

confidence: 99%

“…Now, Teixeira et al [2003] found that corneal epithelial cells elongate and align along silicone ridges 70 nm wide and 150 nm deep. Reduced fi broblast adhesion was observed using nanosized pits on polymethylmethacrylate (PMMA, 35-120 nm in diameter, pitch 100-300 nm) Martines et al, 2004]. On 13-nm-high islands produced by demixing of polymers, fi broblasts showed increased cell spreading and upregulation of gene expression [Dalby et al, 2002].…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Monsees¹,

Barth²,

Tippelt³

et al. 2005

Cells Tissues Organs

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To test nanosize surface patterning for application as implant material, a suitable titanium composition has to be found first. Therefore we investigated the effect of surface chemistry on attachment and differentiation of osteoblast-like cells on pure titanium prepared by pulsed laser deposition (TiPLD) and different Ti alloys (Ti6Al4V, TiNb30 and TiNb13Zr13). Early attachment (30 min) and alkaline phosphatase (ALP) activity (day 5) was found to be fastest and highest, respectively, in cells grown on TiPLD and Ti6Al4V. Osteoblasts seeded on TiPLD produced most osteopontin (day 10), whereas expression of this extracellular matrix protein was an order of magnitude lower on the TiNb30 surface. In contrast, expression of the corresponding receptor, CD44, was not influenced by surface chemistry. Thus, TiPLD was used for further experiments to explore the influence of surface nanostructures on osteoblast adhesion, differentiation and orientation. By laser-induced oxidation, we produced patterns of parallel Ti oxide lines with different widths (0.2–10 µm) and distances (2–20 and 1,000 µm), but a common height of only 12 nm. These structures did not influence ALP activity (days 5–9), but had a positive effect on cell alignment. Two days after plating, the majority of the focal contacts were placed on the oxide lines. The portion of larger focal adhesions bridging two lines was inversely related to the line distance (2–20 µm). In contrast, the portion of aligned cells did not depend on the line distance. On average, 43% of the cells orientated parallel towards the lines, whereas 34% orientated vertically. In the control pattern (1,000 µm line distance), cell distribution was completely at random. Because a significant surplus of the cells preferred a parallel alignment, the nanosize difference in height between Ti surface and oxide lines may be sufficient to orientate the cells by contact guiding. However, gradients in electrostatic potential and surface charge density at the Ti/Ti oxide interface may additionally influence focal contact formation and cell guidance.

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“…[22][23][24][25] A microfluidic system is advantageous for analysis of cell adhesion in various aspects because of small sample consumption, use of multiple parameters, and generation of large shear stress. 26,27 By integrating nanostructured surfaces with a microfluidic system, we herein devise a simple, label-free cell separation and enrichment scheme based on standard soft lithography and microfluidics protocol. The PDMS microfluidic device consists of four branch channels, each of which contains flat or polymeric nanostructures on the bottom (400 nm pillars, 400 nm perpendicular, and 400 nm parallel lines, respectively) to increase the difference in cell adhesion.…”

Section: Introductionmentioning

confidence: 99%

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

et al. 2007

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A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e.g., one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 ml min 21 . The fraction of MCF7 cells was increased from 0.36 ¡ 0.04 to 0.83 ¡ 0.04 under these optimized conditions.

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A Parallel-Plate Flow Chamber to Study Initial Cell Adhesion on a Nanofeatured Surface

Cited by 69 publications

References 37 publications

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

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