A particle-bound auxin receptor from tobacco pith callus

Vreugdenhil, D.; Burgers, A.; Libbenga, K. R.

doi:10.1016/0304-4211(79)90016-6

Cited by 35 publications

(5 citation statements)

References 16 publications

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“…The pH optimum for the 120 K membrane fraction is 4.0 (Fig. 2), while the pH optima for vegetative tissues range from 5.0 in zucchini (12) and tobacco pith callus (32), 5.5 in com (1,23), to 8.0 in mung bean (13,33). The physiological relevance ofthis low pH optimum is unknown, but fruits in general appear to have a low pH optimum for auxin binding as evidenced by pH optima of 3.75 for cucumber fruit and 3.5 for bean and tomato fruit CM fraction (16,18).…”

Section: Discussionmentioning

confidence: 99%

Demonstration of Auxin Binding to Strawberry Fruit Membranes

Narayanan¹,

Mudge²,

Poovaiah³

1981

Plant Physiol.

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Section: Discussionmentioning

confidence: 99%

Demonstration of Auxin Binding to Strawberry Fruit Membranes

Narayanan¹,

Mudge²,

Poovaiah³

1981

Plant Physiol.

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“…Auxin's effect on mRNAs or specific proteins, rapid enough to cause cell elongation, however, could not be detected by techniques available at that time even though auxin fully stimulates growth within 10 to 25 min (22,33,90,95). No experimental evidence convincingly demonstrated the direct action of the hormone on a transport system such as proton pumping (39,100), although the inhibition of auxin induced proton secretion by protein synthesis inhibitors (14,16,17,71) indicated that auxin may act indirectly by stimulating a proton pump through newly synthesized polypeptides which could mediate the growth process in any of several ways. No experimental evidence convincingly demonstrated the direct action of the hormone on a transport system such as proton pumping (39,100), although the inhibition of auxin induced proton secretion by protein synthesis inhibitors (14,16,17,71) indicated that auxin may act indirectly by stimulating a proton pump through newly synthesized polypeptides which could mediate the growth process in any of several ways.…”

Section: Introductionmentioning

confidence: 99%

Rapid Gene Regulation by Auxin

Theologis

1986

Annu. Rev. Plant. Physiol.

189

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“…These include an increase in the microviscosity of lipid bilayers (17) or plasma membrane vesicles (13) and changes in electrostatic surface properties of protoplasts (29). Finally, a specific binding of auxin to membranes isolated from maize coleoptiles (9) and tobacco cell suspensions (30) has been described.…”

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confidence: 99%

Auxin Effect on the Transmembrane Potential Difference of Wild-Type and Mutant Tobacco Protoplasts Exhibiting a Differential Sensitiity to Auxin

Ephritikhine¹,

Barbier‐Brygoo²,

Müller³

et al. 1987

Plant Physiol.

100

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The effects of 1-naphthaleneacetic acid (NAA) and other auxin analogs on the transmembrane potential difference (Em) were compared on tobacco protoplasts isolated from two genotypes differing in their sensitivity to auxins. For both types, NAA modifies Em by inducing at low doses a hyperpolarization, the amplitude of which increased with auxin concentration. Above an optimal concentration this hyperpolarization was reduced and even nullified. However, for the mutant type, this electrical response was shifted toward higher NAA concentrations, as its growth response. In the presence of structural analogs of auxin which have been showed to modify the dose-response curve for growth, the Em was altered: the growth-stimulatory molecule (picloram) initiated hyperpolarization, whereas the growth-inhibitory substance (4-bromophenylacetic acid) caused depolarization. These results provide evidence for a specific action of auxin at the membrane level related to its biological activity.There is considerable evidence for interactions between auxin and membranes. Ultrastructural alterations were induced by auxin in isolated and in situ soybean plasma membranes (22). Modifications of the physical properties of membranes after auxin treatment have been reported. These include an increase in the microviscosity of lipid bilayers (17) or plasma membrane vesicles (13) and changes in electrostatic surface properties of protoplasts (29). Finally, a specific binding of auxin to membranes isolated from maize coleoptiles (9) and tobacco cell suspensions (30) tiles (24).Causal links between these effects of auxin on membrane properties and the biological activity of auxin are difficult to establish. Very recently, binding of auxin at the plasmalemma of epidermal cells has been reported to be related to the elongation and the curling of corn coleoptiles (18). In other studies, the auxin-induced proton extrusion has been correlated to the elongation rate of shoots (12, 14, 25) and the control of root growth (10, 21) on the basis of similarities between the timing of the responses, the dependency upon auxin concentration, and the specificity of the response to auxin analogs. Such results provide evidence for the existence of a relationship between the action of auxin on membranes and the overall activity of this hormone on cell enlargement. Up to now, the involvement of hormoneinduced modifications of membranes into the control of cell division and differentiation has not been directly demonstrated.A novel opportunity to study the basis ofthe biological activity ofauxins at the cellular level was recently offered by the selection of a NAA-tolerant mutant from tobacco mesophyll protoplasts (5, 23). The wild-type (clone D8) and the mutant (clone 36) differ in their sensitivity to auxins as measured by the ability of the cells derived from protoplasts to proliferate in the presence of NAA. In this paper we test the hypothesis that the wild-type and mutant protoplasts differ in their membrane properties. A comparative study of the effe...

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A particle-bound auxin receptor from tobacco pith callus

Cited by 35 publications

References 16 publications

Demonstration of Auxin Binding to Strawberry Fruit Membranes

Demonstration of Auxin Binding to Strawberry Fruit Membranes

Rapid Gene Regulation by Auxin

Auxin Effect on the Transmembrane Potential Difference of Wild-Type and Mutant Tobacco Protoplasts Exhibiting a Differential Sensitiity to Auxin

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