A Particularly Sensitive Modification of the Peroxidase Reaction

Ld, Leder

doi:10.1159/000208694

Cited by 13 publications

(3 citation statements)

References 3 publications

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“…The following methods were applied: Pappenhelm's stain, the cytochemical stains for peroxidase [15], non-specific alpha-naphthol acetate esterase, naphthol AS acetate esterase [14], and naphthol AS-D chloroacetate esterase [13]. In addition, the PAS-reaction [12] was performed.…”

Section: Methodsmentioning

confidence: 99%

Relapsing acute non-lymphatic leukemia with chaning phenotypes

1985

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Morphological and cytochemical findings in a case of acute myeloid leukemia (AML) with 3 relapses are described. The disease began as a subtype M4 with atypical eosinophils according to the French-American-British (FAB)-classification. At the time of the first relapse, the phenotype had converted into an M2-subtype, while the second relapse presented the picture of an M1-type of acute myeloid leukemia with non-granulated peroxidase-positive blasts. When the third relapse occurred, the diagnosis was again an M4-type of acute myeloid leukemia. The observed changes of the phenotype can be interpreted as an expression of tumor cell heterogeneity, even though the observed emergence of new leukemic clones would be attributed entirely or in part to effects of the applied cytostatic drugs, because tumor cell heterogeneity concerns also the response to therapy, which may result in a growth advantage for certain leukemic subpopulations. In so far, AML behaves like all other human malignant neoplasias. In addition, morphological changes in leukemias such as are reported here, have been observed long before the introduction of cytostatic drugs. Also, the validity of the FAB-classification is discussed in the light of the presented observation. It is concluded that the FAB-classification is not a classification in a strict sense, but merely a crude and simplistic list of subtypes of acute myeloproliferative diseases that is difficult to reproduce and that disregards major aspects of this group of diseases, for instance tumor cell heterogeneity.

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Section: Methodsmentioning

confidence: 99%

Relapsing acute non-lymphatic leukemia with chaning phenotypes

1985

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“…Following a fixation for 3 s at 20°C in formalinmethanol (1:10) the slides were rinsed in tap water, air-dried, subjected to the peroxidase-reaction (Leder 1970) with 3-amino-9-ethyl-carbazol (Sigma, USA), counterstained with hemalum and covered with Kaiser's glycerine gelatin (Merck, Germany).…”

Section: Histological and Immunohistochemical Investigationsmentioning

confidence: 99%

Ein Plasmozytomfall mit immunhistochemisch verschiedenen Zellpopulationen und seine Bedeutung f�r die immunhistochemische Monoclonalit�tsbestimmung von Lymphomzellen

Metz

Leder

1980

Klin Wochenschr

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A case of myeloma showed histologically a monotonous pattern of relatively mature myeloma cells and an IgG-kappa paraproteinemia. In contrast to ordinary stains, the immunoperoxidase reactions for kappa and gamma gave inhomogeneous results. Light and heavy chains were formed by clusters of different subpopulations of myeloma cells. Serial sections showed that kappa-positive clusters were gamma-negative and vice versa. Thus, this morphologically homogeneous myeloma turned out to be immunohistochemically heterogeneous. We assume that two subpopulations have developed that produced separately kappa- and gamma-chains. Therefore, these chains might have been circulating separately and not as complete IgG-kappa molecules. Guided by our findings, the results of other investigators are discussed. Some considerations on the validity of immunohistochemical demonstration of tumor cell monoclonality are given implicating the diagnostic equivocation of immunohistochemistry for non-Hodgkin's lymphomas, especially for so-called "immunocytomas".

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“…The results of the 2 methods were in accordance with each other, but the individual cells were easier to identify in the preparations settled in the sedimen tation chamber. In the peripheral blood, bone marrow, and ascites, the following re actions were performed: peroxydase, in part after Sato-Sekiye, in part after Leder [7]; non-specific esterase, according to Burstone [2], with naphthol AS-D chloroacetate substrate [10]; alkaline phosphatase, according to G omori and T akamatsu; as well as Sudan black B staining, after Sheehan and Storey [12], Histological examinations. The organs of the primary and transplanted leukaemic animals (liver, spleen, lymph nodes, thymus, bone marrow, lungs, kidneys and ad renals) were embedded and the sections stained with haematoxylin-eosin (HF.…”

Section: G Al/s Omfai/szlni Irmaymentioning

confidence: 99%

Transplantable Myeloid Rat Leukaemia Induced by 7,12-Dimethylbenz(a)anthracene

F¹,

Somfai²,

Szentirmay³

1973

Acta Haematol

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With repeated intravenous administrations of DMBA transplantable myeloid rat leukaemia has been induced. In the course of transplantations 2 forms have developed: (1) the subacute form maintained with liver and spleen cell suspensions yielding a true leukaemic blood picture; (2) the acute ascitic form without leukaemic blood picture. The subacute form of leukaemia following serial transplantation has kept its original character.

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A Particularly Sensitive Modification of the Peroxidase Reaction

Cited by 13 publications

References 3 publications

Relapsing acute non-lymphatic leukemia with chaning phenotypes

Relapsing acute non-lymphatic leukemia with chaning phenotypes

Ein Plasmozytomfall mit immunhistochemisch verschiedenen Zellpopulationen und seine Bedeutung f�r die immunhistochemische Monoclonalit�tsbestimmung von Lymphomzellen

Transplantable Myeloid Rat Leukaemia Induced by 7,12-Dimethylbenz(a)anthracene

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