A Pathogen-Specific Cargo Delivery Platform Based on Mesoporous Silica Nanoparticles

Abstract

We present a synthetic approach to a highly pathogen-selective detection and delivery platform based on the interaction of an antibody nanovalve with a tetrasaccharide from the O-antigen of the lipopolysaccharide (LPS) of Francisella tularensis bacteria, a Tier 1 Select Agent of bioterrorism. Different design considerations are explored, and proof-of-concept for highly pathogen-specific cargo release from mesoporous silica nanoparticles is demonstrated by comparisons of the release of a signal transducer and model drug by LPS from F. tularensis vs Pseudomonas aeruginosa and by F. tularensis live bacteria vs the closely related bacterium Francisella novocida. In addition to the specific response to a biowarfare agent, treatment of infectious diseases in general could benefit tremendously from a delivery platform that releases its antibiotic payload only at the site of infection and only in the presence of the target pathogen, thereby minimizing off-target toxicities.

Interfacial Assembly of Mesoporous Silica‐Based Optical Heterostructures for Sensing Applications

“…In the MSMs‐based optical sensing systems, specific interactions between analyte and optical receptors that immobilized in the heterostructures are the most common operating principles. Until now, various specific interaction mechanisms, such as metal coordination, molecular targeting, nucleic acid pairing, and antigen–antibody recognition, were utilized in the MSMs sensor system which reported its optical property change upon the presence of analyte . Qiu et al prepared a bis(rhodamine)‐derived‐bridged periodic mesoporous organosilicas (PRhPMOs) showing dual‐fluorescent emission at λ = 550 and 623 nm.…”

Mesoporous Silica‐Based Materials with Bactericidal Properties

“…Moreover, the pore‐blockers should respond to appropriate external or internal triggers to close or open the well‐defined pores, causing on‐demand intelligent cargo delivery . Various porekeepers including polymers (e.g., polymer poly(2‐vinylpyridine) (PVP), poly(N‐succinimidyl acrylate), poly(2‐dimethylaminoethyl methacrylate), poly(acrylic acid) brush), host–guest assemblies (e.g., Cyclodextrins, cucurbit,uril, pillararenes), inorganic nanomaterials (e.g., Au NPs, quantum dots, Ag NPs, cerium oxide NPs, manganese oxide NPs, and reduced graphene), and biomacromolecules (e.g., peptides, nucleic acids, saccharides, and proteins) have been employed under certain internal or external stimuli, such as pH, temperature, light, redox potential, ultrasound, small molecules, biomolecules, and or a combination of these stimuli, to achieve controllable DDSs based on mesoporous silica nanoparticles (MSNs) …”

“…This is usually achieved by decorating external surface of the NPs with targeting ligands. In drug delivery to bacteria/biofilms, however, targeted‐MSNs have been scarcely used as drug carrier . As an example, the disaccharide trehalose has been used as targeting ligand for targeted drug delivery to mycobacteria (vide infra).…”

“…In drug delivery to bacteria/biofilms, however, targeted-MSNs have been scarcely used as drug carrier. [89,90,123,124] As an example, the disaccharide trehalose has been used as targeting ligand for targeted drug delivery to mycobacteria (vide infra). Moreover, one of the biggest challenges when using antimicrobials is AMR.…”

Ultrasound responsive mesoporous silica nanoparticles for biomedical applications

“…49 106,107,108,108,109,110,111,112,113,114 or specific antibodies to tumour cells. 115,116 All this stimuli-responsive MSNs platforms have been reviewed extensively somewhere else. 38,117 ,118,119,120,121,122,123,124,125,126,127,128,129 This feature article will focus on the recent research carried out on ultrasound sensitive MSNs, one of the newest and most efficient stimuli employed with MSNs.…”