A PCR-based method for genetic sex identification and evidence of the XX/XY sex determination system in largemouth bass (Micropterus salmoides L.)

Du, Jianguo; Zhou, Jiahui; Li, Shengjie; Shao, Jiaqi; Jiang, Peng; Dong, Chuanju; Bai, Junjie

doi:10.1016/j.aquaculture.2021.737220

Cited by 30 publications

(8 citation statements)

References 45 publications

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“…However, few studies have been reported about the InDels in largemouth bass. Only a 66-bp deletion in the growth hormone releasing hormone gene was reported to be related to livability during embryonic development ( Ma et al, 2014 ), and two InDels were identified as sex-specific markers in largemouth bass ( Du et al, 2021 ). In this study, InDels between NB and FB were analyzed for the first time.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis and development of molecular markers for the identification of largemouth bass (Micropterus salmoides L.) based on whole-genome re-sequencing

Shao

et al. 2022

Front. Genet.

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Largemouth bass (Micropterus salmoides L.) is generally considered to comprise two subspecies, Florida bass (M. floridanus) and Northern Largemouth bass (M. salmoides), which have biological characteristic differences because of their geographical distribution. In this study, whole-genome re-sequencing was performed among 10 Florida and 10 Northern largemouth bass, respectively. In total, 999,793 SNPs and 227,797 InDels were finally identified, and 507,401 SNPs (50.75%) and 116,213 InDels (51.01%) were successfully mapped to annotated 18,629 genes and 14,060 genes, respectively. KEGG classification indicated that most of these genes were focused on the pathways including signal transduction, transport and catabolism, and endocrine system. Genetic diversity analysis indicated that Florida largemouth bass had higher genetic diversity than Northern largemouth bass, indicating that the germplasm quality of Northern largemouth bass had markedly reduced in China. To examine the accuracies of the identified markers, 23 SNPs and eight InDels (the insertions or deletions of more than 45 bp) were randomly selected and detected among Florida largemouth bass, Northern largemouth bass, and their F1 hybrids. The detection efficiencies of all the markers were higher than 95%; nineteen SNPs and three InDels could accurately distinguish the two subspecies and their F1 hybrids with 100% efficiencies. Moreover, the three InDel markers could clearly distinguish the two subspecies and their F1 hybrids with a PCR-based agarose gel electrophoresis. In conclusion, our study established a simple PCR-based method for the germplasm identification of largemouth bass, which will be useful in the germplasm protection, management, and hybridization breeding of largemouth bass.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis and development of molecular markers for the identification of largemouth bass (Micropterus salmoides L.) based on whole-genome re-sequencing

Shao

et al. 2022

Front. Genet.

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“…The production of all-male sh holds signi cant value for the sustainable development of the LMB aquatic industry. Previously, we reported on the identi cation of sex markers (Du et al 2021) and investigated sex differentiation characteristics and sex reversal in LMB (Zhou 2021). We also found that the expression of the female sex-related gene Cyp19a1a began at 20 days post-hatching in LMB (Du et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we reported on the identi cation of sex markers (Du et al 2021) and investigated sex differentiation characteristics and sex reversal in LMB (Zhou 2021). We also found that the expression of the female sex-related gene Cyp19a1a began at 20 days post-hatching in LMB (Du et al 2021). These results demonstrated that this gene plays an important role in the gonad development of female LMB.…”

Section: Introductionmentioning

confidence: 99%

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Zhang,

Tian,

Han

et al. 2024

Fish Physiol Biochem

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To investigate the regulatory role of the Cyp19a1b aromatase gene in the sexual differentiation of largemouth bass (Micropterus salmoides, LMB), we obtained the full-length cDNA sequence of Cyp19a1b using rapid ampli cation of cDNA ends technique. Tissue expression characteristics and feedback with 17-β-estradiol (E 2 ) were determined using quantitative real-time PCR (qRT-PCR), while gonad development was assessed through histological section observations. The cDNA sequence of LMB Cyp19a1b was found to be1950 base pairs (bp) in length, including a 5' untranslated region of 145 bp, a 3' untranslated region of 278 bp, and an open reading frame encoding a protein consisting of 1527 bp that encoded 508 amino acids. The qRT-PCR results indicated that Cyp19a1b abundantly expressed in the brain, followed by the gonads, and its expression in the ovaries was signi cantly higher than that observed in the testes.(P < 0.05). After feeding sh with E 2 for 30 days, the expression of Cyp19a1b in the pseudo-females gonads (XY-F) was signi cantly higher than that in males (XY-M) (P < 0.05), whereas expression did not differ signi cantly between female (XX-F) and XY-M sh (P > 0.05). Although the expression of Cyp19a1b in XY-F and XX-F sh was not signi cantly different after 60 days (P > 0.05), both exhibited signi cantly higher levels than that of XY-M sh (P < 0.05). Histological sections analysis showed the presence of oogonia in both XY-F and XX-F sh at 30 days, while spermatogonia were observed in XY-M sh. At 60 days, primary oocytes were abundantly observed in both XY-F and XX-F sh, while a few spermatogonia were visible in XY-M sh. At 90 days, the histological sections results showed that a large number of oocytes were visible in XY-F and XX-F sh. Additionally, the gonads of XY-M sh contained numerous spermatocytes. These results suggest that Cyp19a1b plays a pivotal role in the development of ovaries and nervous system development in LMB.

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“…In addition to facilitating the development of TSC carrying fish, Sex‐ID markers are a prerequisite for understanding the evolution of sex chromosomes and can aid in the discovery of SD genes (Fowler & Buonaccorsi, 2016; Lamatsch et al ., 2015). The SD systems within the Centrarchidae family vary based on species and even population (Du et al ., 2021; Gomelsky et al ., 2002; Shen et al ., 2016; Wang et al ., 2018). These SD systems range from seemingly simple and chromosomal, such as the XX/XY SD system of largemouth bass Micropterus salmoides (Du et al ., 2021), to more complicated systems, such as those in bluegill Lepomis macrochirus , where increased water temperatures can lead to male‐skewed populations (Shen et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The SD systems within the Centrarchidae family vary based on species and even population (Du et al, 2021;Gomelsky et al, 2002;Shen et al, 2016;Wang et al, 2018). These SD systems range from seemingly simple and chromosomal, such as the XX/XY SD system of largemouth bass Micropterus salmoides (Du et al, 2021), to more complicated systems, such as those in bluegill Lepomis macrochirus, where increased water temperatures can lead to male-skewed populations (Shen et al, 2016). Although the morphologies of L. cyanellus chromosomes have been described (Ohno, 1970;Roberts, 1964), sex chromosomes were not identified, the SD system of L. cyanellus is still unknown and no Sex-ID markers have been developed for this species.…”

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confidence: 99%

Sex‐specific markers undetected in green sunfish Lepomis cyanellus using restriction‐site associated DNA sequencing

Teal¹,

Coykendall

Campbell

et al. 2022

Journal of Fish Biology

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We used restriction‐site associated DNA sequencing for SNP discovery and genotyping of known‐sex green sunfish Lepomis cyanellus DNA samples to search for sex‐diagnostic single nucleotide polymorphisms (SNPs) and restriction‐site associated sequences present in one sex and absent in the other. The bioinformatic analyses discovered candidate SNPs and sex‐specific restriction‐site associated sequences that fit patterns of male or female heterogametic sex determination systems. However, when primers were developed and tested, no candidates reliably identified phenotypic sex. The top performing SNP candidate (ZW_218) correlated with phenotypic sex 63.0% of the time and the presence‐absence loci universally amplified in both sexes. We recommend further investigations that interrogate a larger fraction of the L. cyanellus genome. Additionally, studies on the effect of temperature and rearing density on sex determination, as well as breeding of sex‐reversed individuals, could provide more insights into the sex determination system of L. cyanellus.

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A PCR-based method for genetic sex identification and evidence of the XX/XY sex determination system in largemouth bass (Micropterus salmoides L.)

Cited by 30 publications

References 45 publications

Genetic diversity analysis and development of molecular markers for the identification of largemouth bass (Micropterus salmoides L.) based on whole-genome re-sequencing

Genetic diversity analysis and development of molecular markers for the identification of largemouth bass (Micropterus salmoides L.) based on whole-genome re-sequencing

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Sex‐specific markers undetected in green sunfish Lepomis cyanellus using restriction‐site associated DNA sequencing

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