A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019

Cai, Xue-Fei; Chen, Juan; Hu, Jie; Long, Quanxin; Deng, Haijun; Liu, Ping; Fan, Kai; Liao, Pu; Liu, Beizhong; Wu, Guicheng; Chen, Yao-Kai; Li, Zhijie; Wang, Kun; Zhang, Xiaoli; Tian, Weizhong; Xiang, Junyi; Du, Hongxin; Wang, Jing; Hu, Yuan; Tang, Ni; Lin, Yong; Ren, Ji-Hua; Huang, Luyi; Wei, Jie; Gan, Chun-Yang; Chen, Yanmeng; Gao, Qi; Chen, Amei; He, Chaojun; Wang, Daoxin; Hu, Ping; Zhou, Fachun; Huang, Aiping; Wang, Deqiang

doi:10.1093/infdis/jiaa243

Cited by 152 publications

(135 citation statements)

References 5 publications

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“…Recently, a peptide-based magnetic chemiluminescence enzyme immunoassay was developed for the diagnosis of COVID-19 [75]. The test has high throughput and can perform simultaneous clinical tests for other biomarkers, such as C-reactive protein (CRP), which should also be tracked in COVID-19 suspects [76].…”

Section: Immunoassays For Antibody To Virusmentioning

confidence: 99%

Review of analytical performance of COVID-19 detection methods

et al. 2020

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In the recent SARS-CoV-2 pandemic, public health experts have emphasized testing, tracking infected people, and tracing their contacts as an effective strategy to reduce the spread of the virus. Several diagnostic methods are reported for detecting the coronavirus in clinical, research, and public health laboratories. Some tests detect the infection directly by detecting the viral RNA and other tests detect the infection indirectly by detecting the host antibodies. A diagnostic test during the pandemic should help make an appropriate clinical decision in a short period of time. Recently reported diagnostic methods for SARS-CoV-2 have varying throughput, batching capacity, requirement of infrastructure setting, analytical performance, and turnaround times ranging from a few minutes to several hours. These factors should be considered while selecting a reliable and rapid diagnostic method to help make an appropriate decision and prompt public health interventions. This paper reviews recent SARS-CoV-2 diagnostic methods published in journals and reports released by regulatory agencies. We compared the analytical efficiency including limit of detection, sensitivity, specificity, and throughput. In addition, we also looked into ease of use, affordability, and availability of accessories. Finally, we discuss the limitations of the methods and provide our perspectives on priorities for future test development.

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Section: Immunoassays For Antibody To Virusmentioning

confidence: 99%

Review of analytical performance of COVID-19 detection methods

et al. 2020

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“…Chemiluminesence immuno-assay is one such an approach that has been popularly used in the detection of infectious diseases. Recent studies have demonstrated the application of this method for SARS-CoV-2 [199] , [200] , [201] . Cai et al [200] demonstrated a peptide-based magnetic chemiluminesence enzyme immuno-assay for detecting SARS-CoV-2 antibodies against ORF1a/b, N and S proteins.…”

Section: Rising Test Strategies For Covid-19 Diagnosismentioning

confidence: 99%

Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods

Bukkitgar

Shetti

Aminabhavi³

2021

Chemical Engineering Journal

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“…Although some studies confirm this trend, combining IgG and IgM signals gives even better signals (vide infra), especially that the shedding routes are multiple [ 178 ]. It is also possible to detect SARS-CoV-2 antibodies present in serum samples from people infected with other respiratory pathogens using a peptide that is attached to magnetic beads to act as the immunosorbent in a chemiluminescence enzyme immunoassay [ 193 ] with a similar magnitude of sensitivity (82.52%). In a similar way, Ashtari et al reported the use of hybrid magnetic NPs coated with amino-modified silica (ASMNPs) fabricated by water-in-oil microemulsion method for the improved recovery of target single-stranded DNA (ssDNA) macromolecules [ 194 ].…”

Section: Diagnostics and Biosensors For Covid-19mentioning

confidence: 99%

Biomedical Science to Tackle the COVID-19 Pandemic: Current Status and Future Perspectives

et al. 2020

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The coronavirus infectious disease (COVID-19) pandemic emerged at the end of 2019, and was caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which has resulted in an unprecedented health and economic crisis worldwide. One key aspect, compared to other recent pandemics, is the level of urgency, which has started a race for finding adequate answers. Solutions for efficient prevention approaches, rapid, reliable, and high throughput diagnostics, monitoring, and safe therapies are needed. Research across the world has been directed to fight against COVID-19. Biomedical science has been presented as a possible area for combating the SARS-CoV-2 virus due to the unique challenges raised by the pandemic, as reported by epidemiologists, immunologists, and medical doctors, including COVID-19’s survival, symptoms, protein surface composition, and infection mechanisms. While the current knowledge about the SARS-CoV-2 virus is still limited, various (old and new) biomedical approaches have been developed and tested. Here, we review the current status and future perspectives of biomedical science in the context of COVID-19, including nanotechnology, prevention through vaccine engineering, diagnostic, monitoring, and therapy. This review is aimed at discussing the current impact of biomedical science in healthcare for the management of COVID-19, as well as some challenges to be addressed.

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A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019

Cited by 152 publications

References 5 publications

Review of analytical performance of COVID-19 detection methods

Review of analytical performance of COVID-19 detection methods

Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods

Biomedical Science to Tackle the COVID-19 Pandemic: Current Status and Future Perspectives

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