A peptide-mediated, multilateral molecular dialogue for the coordination of pollen wall formation

Truskina, Jekaterina; Brück, Stefanie; Stintzi, Annick; Boeuf, Sophy; Doll, Nicolas M.; Fujita, Satoshi; Geldner, Niko; Schaller, Andreas; Ingram, Gwyneth

doi:10.1073/pnas.2201446119

Cited by 25 publications

(23 citation statements)

References 37 publications

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“…2 A), indicating that this protein cannot freely diffuse from the tapetum to the middle layer. Similarly, when we expressed the free mTQ2 in the middle layer under the GSO2 promoter, which is active in the middle layer prior to the onset of meiosis and up to the point of tapetum degeneration ( Truskina et al, 2022 ), no fluorescent signal was detected in the tapetum ( Fig. 2 B), although fluorescence was detected in the endothecium and epidermis.…”

Section: Resultsmentioning

confidence: 98%

“…We used a GFP variant, mTurquoise2 (mTQ2), which retains its fluorescent properties in various pH conditions and is thus suitable for imaging in both the symplast and apoplast ( Stoddard and Rolland, 2019 ). Free mTQ2 expression was driven by the AMS promoter, which is active in tapetum cells from meiosis onwards ( Truskina et al, 2022 ). mTQ2 signal was detected exclusively in the tapetum cells from meiosis onwards ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Based on its position, the PTS must permit the diffusion of nutrients such as sugars and amino acids from the mother plant to the developing pollen. In addition, our recent work suggests that the PTS is permeable to small peptides that are perceived in the middle layer and coordinate tapetum and pollen grain development (Truskina et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…The pPAL1- are described in (Andersen et al, 2021). The pAMS-NLS-3xmVenus and pGSO2-NLS-3xmVenus reporter lines are described in (Truskina et al, 2022)…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Anther development in Arabidopsis thaliana involves symplastic isolation and apoplastic gating of the tapetum-middle layer interface

et al. 2022

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During flowering plant reproduction, anthers produce pollen grains, the development of which is supported by the tapetum, a nourishing maternal tissue that also contributes non-cell autonomously to the pollen wall, the resistant external layer on the pollen surface. How the anther restricts movement of the tapetum derived pollen wall components, whilst allowing metabolites such as sugars and amino acids to reach the developing pollen, remains enigmatic. Here we experimentally show that in Arabidopsis thaliana, the tapetum and developing pollen are symplastically isolated from each other, and from other sporophytic tissues, from meiosis onwards. We show that the peritapetal strip (PTS), an apoplastic structure, separates the tapetum and the pollen grains from other anther cell layers and can prevent the apoplastic diffusion of fluorescent proteins, again from meiosis onwards. The formation and selective barrier functions of the PTS require two NADPH oxidases, RBOHE and RBOHC, which play a key role in pollen formation. Together our results suggest that, together with symplastic isolation, gating of the apoplast around the tapetum may help generate metabolically distinct anther compartments.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The pPAL1- are described in (Andersen et al, 2021). The pAMS-NLS-3xmVenus and pGSO2-NLS-3xmVenus reporter lines are described in (Truskina et al, 2022)…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Anther development in Arabidopsis thaliana involves symplastic isolation and apoplastic gating of the tapetum-middle layer interface

et al. 2022

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“…From the tetrads, haploid microspores were released. In these, the formation of a large vacuole was observed and, in the periphery, primexine was observed, which serves as a scaffold for pollen wall formation 35 (Fig. 3 -Stage 7).…”

Section: Resultsmentioning

confidence: 99%

Morphological and anatomical characterization of yellow diploid potato flower for effective breeding program

Bohorquez-Quintero

Galvis-Tarazona

Moreno

et al. 2022

Sci Rep

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The diploid yellow potato (Solanum tuberosum L. Phureja Group) is an important plant genetic resource. In this study, we report for the first time the characterization of anther development and pollen formation in the cultivar Criolla Colombia. The description of morphological and histological characters of buds and flowers at different developmental stages permitted to identify ten main stages, from the differentiation of the male cells of the sporangium, meiosis, microspores formation and maturation, to the release of mature pollen. In addition, the results provide a graphic guide of the development of the anther, through the sequential and orderly formation of the epidermis, the endothecium, the middle layer and the nutritive layer or tapetum. This microanatomical information will be useful for work focused on androgenesis and identification of gene regulation in floral biology and gamete formation. Therefore, this study determined that to efficiently obtain haploids, flower buds between 5 and 8.9 mm long (stage 6 to 8) should be used, in which tetrads and microspores are in the early uninucleate and binucleate stage.

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Single‐Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers

Li,

Ma,

et al. 2023

Advanced Science

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Plant anthers are composed of different specialized cell types with distinct roles in plant reproduction. High temperature (HT) stress causes male sterility, resulting in crop yield reduction. However, the spatial expression atlas and regulatory dynamics during anther development and in response to HT remain largely unknown. Here, the first single‐cell transcriptome atlas and chromatin accessibility survey in cotton anther are established, depicting the specific expression and epigenetic landscape of each type of cell in anthers. The reconstruction of meiotic cells, tapetal cells, and middle layer cell developmental trajectories not only identifies novel expressed genes, but also elucidates the precise degradation period of middle layer and reveals a rapid function transition of tapetal cells during the tetrad stage. By applying HT, heterogeneity in HT response is shown among cells of anthers, with tapetal cells responsible for pollen wall synthesis are most sensitive to HT. Specifically, HT shuts down the chromatin accessibility of genes specifically expressed in the tapetal cells responsible for pollen wall synthesis, such as QUARTET 3 (QRT3) and CYTOCHROME P450 703A2 (CYP703A2), resulting in a silent expression of these genes, ultimately leading to abnormal pollen wall and male sterility. Collectively, this study provides substantial information on anthers and provides clues for heat‐tolerant crop creation.

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A peptide-mediated, multilateral molecular dialogue for the coordination of pollen wall formation

Cited by 25 publications

References 37 publications

Anther development in Arabidopsis thaliana involves symplastic isolation and apoplastic gating of the tapetum-middle layer interface

Anther development in Arabidopsis thaliana involves symplastic isolation and apoplastic gating of the tapetum-middle layer interface

Morphological and anatomical characterization of yellow diploid potato flower for effective breeding program

Single‐Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers

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