A peptide translated from <scp>circPPP1R12A</scp> promotes the malignancy of <scp>non‐small</scp> cell lung cancer cells through <scp>AKT</scp> signaling pathway

Zhao, Wenxuan; Xue, Yali; Zhang, Yandan; Zhu, Yao; Chen, Zixuan; Zhao, Xiaodong

doi:10.1002/jcla.24644

Cited by 7 publications

(1 citation statement)

References 47 publications

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“…Subsequently, circPPP1R12A was shown to encode a newly identified protein, circPPP1R12A-73aa, which has been previously reported to play a catalytic role in colon cancer (61). Subsequently, Zhao et al (76) found that the expression of circPPP1R12A was also significantly increased in small-cell lung cancer, and its associated protein circPPP73R1A-73aa could promote the proliferation of small-cell lung cancer through the AKT pathway. Therefore, this study also provided useful insights and suggestions for the clinical treatment of NSCLC.…”

Section: Circrna-encoded Proteins and Gastric Cancer (Gc)mentioning

confidence: 99%

Advances in the protein‑encoding functions of circular RNAs associated with cancer (Review)

Yuan

Zhang²,

Cong

2023

Oncol Rep

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Circular RNAs (circRNAs) are a special class of non-coding RNAs that are widely expressed in tissues and cells. Owing to their lack of a 5'-cap and 3'-polyadenylated [poly(A)] tail, they are more structurally stable and difficult to degrade compared with linear RNA. Numerous studies published recently have suggested that circRNAs can encode peptides or proteins through cap-independent translation mechanisms and participate in the occurrence and development of cancer. In the present review, the translation mechanism underlying the encoding of proteins by circRNAs, the biological information tools that are available for predicting translation, and the identification and verification of their translational abilities are summarized and analyzed. Finally, the mechanisms associated with circRNA-encoded proteins or polypeptides in various types of cancer are summarized. In this review and its discussion on circRNAs and their coding function, we hope to provide novel perspectives and possibilities for the treatment of cancer as knowledge in this area is added to and developed in the future. Contents 1. Introduction 2. Translational mechanisms of circRNAs 3. Bioinformatics tools for the analysis of circRNA-encoded proteins 4. Role of circRNA-encoded proteins in cancer 5. Concluding remarks

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Section: Circrna-encoded Proteins and Gastric Cancer (Gc)mentioning

confidence: 99%

Advances in the protein‑encoding functions of circular RNAs associated with cancer (Review)

Yuan

Zhang²,

Cong

2023

Oncol Rep

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A peptide translated from circPPP1R12A promotes the malignancy of non‐small cell lung cancer cells through AKT signaling pathway

Zhao

Xue

Zhang

et al. 2022

Clinical Laboratory Analysis

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Background Recent literature have indicated that the malignancy of cancer cells is modulated by hsa_circ_0000423 (named circPPP1R12A) through the way of translating protein. Herein, we investigated the role and latent mechanisms of circPPP1R12A in Non‐Small Cell Lung Cancer (NSCLC). Methods CircPPP1R12A expression was measured by qRT‐PCR. The malignancy of NSCLC was determined by CCK‐8, TUNEL assay, Wound healing, Transwell and Western blotting assays. The underlying mechanisms of circPPP1R12A were confirmed by Western blotting and qRT‐PCR assays. Results CircPPP1R12A expression in NSCLC tissues was higher than that of neighboring normal tissues. CircPPP1R12A showed an upregulated expression in NSCLC cells. Upregulation of circPPP1R12A could promote the cell viability of NSCLC cells and reduce the apoptosis of NSCLC cells, while it could not promote cell invasion and migration. The reduction of cell viability and apoptosis was discovered in NSCLC cells with the silencing of circPPP1R12A, but circPPP1R12A knockdown does not inhibit cell invasion and migration. There was something interesting that circPPP1R12A encoding protein circPPP1R12A‐73aa was found in NSCLC cells. Mutations in circPPP1R12a‐73AA might disrupt the function of circPPP1ra‐73AA in A549 and H1299 cells. Next, we found that circPPP1R12A caused the increased growth of NSCLC cells by activating AKT signaling pathway. Conclusion In summary, our study proved that NSCLC cell proliferation was promoted by circPPP1R12A‐73aa translated from circPPP1R12A through the AKT pathway, which could throw some light on the understanding of the mechanism of NSCLC.

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Hsa_circ_0000423 promotes colorectal cancer EMT and immune escape by competitive adsorption of miR-369-3p mediating CCND1 expression

Huang,

Jiang,

et al. 2024

Discov Onc

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Background This investigation evaluated the mechanism of hsa_circ_0000423 in colorectal cancer (CRC). Methods The hsa_circ_0000423 gene was identified by bioinformatics analyses of GEO circRNA microarrays, and its expression in CRC was investigated. Based on this, in vitro experiments were conducted. Assays with dual luciferase reporter and RIP were conducted to detect interactions between hsa_circ_0000423, miR-369-3p and CCND1. Cell proliferation was measured by MTT and colony formation assay assays, apoptosis was detected by flow cytometry, migration and invasion were detected by Transwell, and expression of EMT-related proteins was detected by Western Blot. SW480 cells and T cells were co-cultured to assess immune escape. Results hsa_circ_0000423 and CCND1 were elevated in CRC while miR-369-3p was downregulated Silencing hsa_circ_0000423 resulted in reduced CCND1 expression by upregulating miR-369-3p. Overexpressing CCND1 or down-regulating miR-369-3p both interrupted the anti-tumor role of silencing hsa_circ_0000423 on CRC cells. Conclusion Hsa_circ_0000423 promotes CCND1 expression through competitive binding of miR-369-3p and promotes CRC cell development and immune escape.

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A peptide translated from circPPP1R12A promotes the malignancy of non‐small cell lung cancer cells through AKT signaling pathway

Cited by 7 publications

References 47 publications

Advances in the protein‑encoding functions of circular RNAs associated with cancer (Review)

Advances in the protein‑encoding functions of circular RNAs associated with cancer (Review)

A peptide translated from circPPP1R12A promotes the malignancy of non‐small cell lung cancer cells through AKT signaling pathway

Hsa_circ_0000423 promotes colorectal cancer EMT and immune escape by competitive adsorption of miR-369-3p mediating CCND1 expression

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