A Perfusion Procedure (Perf-O-Suction) for Recovery of Schistosome Worms

Adult worm tegumental damage and egg-granulomas in praziquantel-resistant and -susceptible Schistosoma mansoni treated in vivo

“…On day 57 after infection, the mice were treated with a single sub-curative dose of micronized PZQ (300 mg kg 21 ) by oral gavage in an aqueous suspension. At 10 and 30 min and 1, 12, and 24 h after dosing, three mice from each of the groups infected with the two isolates were killed and adult worms recovered immediately by portal perfusion (Radke et al, 1961;Smithers & Terry, 1965). Worms collected in the perfusates were washed several times with saline and fixed for 24 -48 h in 5% glutaraldehyde at 48C.…”

Experience with a perfo-suction system for the recovery of schistosomes from laboratory rodents

“…A perfo-suction system ( Fig. I) has been evolved in this laboratory which incorporates features of systems previously described by other authors (Faust & Meleney, ] Moran, 1961;Cheever & Powers, 1969), combined in such a way as to ensure rapidity of operation and optimal worm recovery. The system is designed to permit sequential perfusion of up to 5 rodents at a time.…”

Study of Schistosoma mansoni isolates from patients with failure of treatment with oxamniquine

“…Thirty of the 60 mice infected with S. mansoni from each of the five patients who presented "failure of treatment" to oxamniquine (denoted isolates Hospital Universitário 1, 2, 3, 4 and 5) were treated with oxamniquine at a dose of 100 mg/kg body weight on the 50th day post-infection using a tube adapted in the laboratory. On the 14th day post-treatment the animals were submitted to perfusion of the portal system and the worms were counted in the liver and mesentery using the technique of Radke et al (1961), in order to determine the number of live worms recovered. The remaining 30 animals (out of a total of 60) were not treated with oxamniquine and were used as controls (isolates HU 1c, 2c, 3c, 4c and 5c).…”

“…Thirty animals (denoted isolates HU 6, 7, 8, 9 and 10) were submitted to treatment with oxamniquine (100 mg/kg body weight by the oral route) on the 50th day after infection. On the 14th day post-infection, the animals were submitted to perfusion of the portal system and the worms were counted in the liver and mesentery by the technique of Radke et al (1961). The 30 remaining mice out of a total of 60 were not treated and formed the control group (Hospital Universitário 6c, 7c, 8c, 9c, and 10c).…”