A perspective on functionalizing colloidal quantum dots with DNA

Samanta, Anirban; Deng, Zhengtao; Liu, Yan; Yan, Hao

doi:10.1007/s12274-013-0367-x

Cited by 33 publications

(27 citation statements)

References 67 publications

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“…We realize that the work here represents only a small fraction of what should be possible, given the rich space of possibilities available using DNA structures. Extensions could include larger networks assembled on origami incorporating far more dyes/FRET steps, increased ranges of donor–acceptor ratios and incorporation of other nanomaterials such as QDs, whose inherent light harvesting and nanoplatform/display capabilities have much to offer in this context 39 40 68 69 . Another exciting avenue involves putting such structures to work for biosensing, charge conversion, molecular logic and computing or even as focusing networks built around a biocompatible scaffold for sensitizing photodynamic therapy agents 2 8 .…”

Section: Discussionmentioning

confidence: 99%

Assembling programmable FRET-based photonic networks using designer DNA scaffolds

et al. 2014

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DNA demonstrates a remarkable capacity for creating designer nanostructures and devices. A growing number of these structures utilize Förster resonance energy transfer (FRET) as part of the device's functionality, readout or characterization, and, as device sophistication increases so do the concomitant FRET requirements. Here we create multi-dye FRET cascades and assess how well DNA can marshal organic dyes into nanoantennae that focus excitonic energy. We evaluate 36 increasingly complex designs including linear, bifurcated, Holliday junction, 8-arm star and dendrimers involving up to five different dyes engaging in four-consecutive FRET steps, while systematically varying fluorophore spacing by Förster distance (R0). Decreasing R0 while augmenting cross-sectional collection area with multiple donors significantly increases terminal exciton delivery efficiency within dendrimers compared with the first linear constructs. Förster modelling confirms that best results are obtained when there are multiple interacting FRET pathways rather than independent channels by which excitons travel from initial donor(s) to final acceptor.

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Section: Discussionmentioning

confidence: 99%

Assembling programmable FRET-based photonic networks using designer DNA scaffolds

et al. 2014

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“…The conjugation method was adopted from previous reports (Figure 1) [46,50]. Briefly, 100 µL of 100 nM MPA-capped, ZnS-shelled QDs (in water) were mixed with 20 µL of 100 µM PTO-DNA (sequence g 9 –T 9 , in water), followed by adding 4.5 µL of 25 mM Zn(NO 3 ) 2 (in water) and 4.5 µL of 50 mM MPA (in water).…”

Section: Methodsmentioning

confidence: 99%

DNA-Based Assembly of Quantum Dots into Dimers and Helices

Zhang

Liedl

2019

Nanomaterials

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Owing to their unique optical properties, colloidal quantum dots (QDs) have attracted much attention as versatile fluorescent markers with broad biological and physical applications. On the other hand, DNA-based assembly has proven to be a powerful bottom-up approach to create designer nanoscale objects and to use these objects for the site-directed arrangement of guest components. To achieve good colloidal stability and accurate positioning of QDs on DNA templates, robust QD surface functionalization is crucial. Here, we present a simple and reliable conjugation method for the direct attachment of DNA molecules to QDs. Phosphorothiolated regions of chimera oligonucleotides are attached and incorporated into a ZnS layer freshly growing in situ on QDs that were rendered water soluble with hydrophilic ligands in a prior step. The reaction can be completed in a 2 mL plastic tube without any special equipment. The utility of these DNA-labeled QDs is demonstrated via prototypical assemblies such as QDs dimers with various spacings and chiral helical architectures.

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“…Other inorganic materials including carbon nanomaterials, magnetic nanoparticles (MNPs), QDs and mesoporous silica nanoparticles (MSNs) have also been explored to functionalize NANDs to fulfill various biomedical goals. Particularly, the hybrid materials coupling the NANDs with carbon nanomaterials (e.g., single walled nanotube and graphene) and QDs offer good opportunities for sensing and imaging .…”

Section: Biomedical Applications Of Functionalized Nandsmentioning

confidence: 99%

Progress in Biomedical Applications of Functionalized Nucleic Acid Nanodevices (NANDs)

2016

ChemNanoMat

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DNA and RNA are building blocks with excellent programmability for nanoscale assembly.O wing to their appealing properties such as precision in bottom-up fabrication, enhanced stabilitya nd cellular internalization, designed nucleic acid nanodevices( NANDs) have been widely explored in biomedical research. Compared with pristine nucleic acids, chemically modifiedN ANDs exhibit aw ider spectrum of applicability.I nt his Focus Review, we highlight recent advances of NANDs functionalized with small molecules, peptides/proteins or inorganic materials, and elucidate their promisingf uture for biomedical application.

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A perspective on functionalizing colloidal quantum dots with DNA

Cited by 33 publications

References 67 publications

Assembling programmable FRET-based photonic networks using designer DNA scaffolds

Assembling programmable FRET-based photonic networks using designer DNA scaffolds

DNA-Based Assembly of Quantum Dots into Dimers and Helices

Progress in Biomedical Applications of Functionalized Nucleic Acid Nanodevices (NANDs)

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