A Perspective on the Control of Mammalian Fertilization by Egg-Activated Ion Channels in Sperm: A Tale of Two Channels1

Florman, Harvey M.; Arnoult, Christophe; Kazam, Imrana G.; Li, Chongqing; O'Toole, Christine M.B.

doi:10.1095/biolreprod59.1.12

Cited by 177 publications

(114 citation statements)

References 45 publications

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“…In other words, this study disagreed with the finding of Zini et al and Vignini et al that the two sperm preparations could involve in the production of ROS which may damage the plasma membrane of the sperm and causing the decrease of ATPase [11,20]. Sperm plasma membrane plays an important role in performing Na + ,K + -ATPase activity to maintain Na + and K + homeostasis by its membrane potential and membrane permeability functions [21,22]. …”

Section: Sperm Parameters In Whole Semen and Prepared Spermcontrasting

confidence: 88%

Sperm Na+, K+-ATPase and Ca2+-ATPase activity: A preliminary study of comparison of swim up and density gradient centrifugation methods for sperm preparation

Lestari

Larasati

Asmarinah

et al. 2018

AIP Conference Proceedings

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Section: Sperm Parameters In Whole Semen and Prepared Spermcontrasting

confidence: 88%

Sperm Na+, K+-ATPase and Ca2+-ATPase activity: A preliminary study of comparison of swim up and density gradient centrifugation methods for sperm preparation

Lestari

Larasati

Asmarinah

et al. 2018

AIP Conference Proceedings

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“…In human sperm GABA can induce the acrosomal reaction [Calogero et al 1996], suggesting that GABA might be a regulatory factor of sperm function. GAT1 could also lead to depolarization of cell membrane, which is important in the initiation of acrosomal reaction of sperm [Mager et al 1993;Florman et al 1998]. Therefore, misregulation of GAT1 might lead to infertility by its affect on sperm development.…”

mentioning

confidence: 99%

Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

Zhang

Gui

Yuan

et al. 2009

Systems Biology in Reproductive Medicine

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The present study was carried out to identify GABA (g-aminobutyric acid) transport protein I (GAT1) in male reproductive organs and to study the effect of GAT1 overexpression on the male reproductive system in GAT1 transgenic mice (TG). Expression and location of GAT1 in testes, epididymis, and sperm of wild-type (WT) mice were identified by immunohistochemistry and western-blot. Histological changes of testes, epididymis, and sperm of transgenic mice overexpressing GAT1 were detected by immunofluorenscent staining and haematoxylin and eosin (HE) staining. GAT1 expression was detected in the testes, epididymis, and sperm of non-transgenic mice. Vacuolization and deformity of spermatogenic cells were observed in the transgenic mice, but the epididymis was unremarkable. Immunofluorenscent staining showed that the number of diastrophic and decapitated sperm increased significantly in transgenic mice to 46.9% from 7.3% in nontransgenic mice. These results suggest that abnormal expression of GAT1 could result in spermiogenesis function injury, sperm paramorphia and dysgenesis.

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“…26 One such cascade is likely to be the activation of sodium/proton (Na 1 /H 1 ) exchange pumps, resulting in intracellular alkalinisation. 26,27 Second messengers include the adenylate cyclasecyclic adenosine monophosphate system, which activates protein kinase A (PKA), leading to the phosphorylation of specific putative proteins involved in acrosomal exocytosis. In addition, the activation of PLCb 1 and/or PLCc leads to an increase in the levels of 1,2-diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP 3 ).…”

Section: Signalling Events During Zp-mediated Acrosomal Exocytosismentioning

confidence: 99%

“…DAG may stimulate protein phosphorylation through PKC, whereas IP 3 may activate intracellular calcium ([Ca 21 ] i ) release through the modulation of IP 3 -sensitive intracellular calcium stores. [25][26][27] Studies with the mouse ZP solubilized by acid disaggregation have shown that the ZP-induced AR is a Ca 21 -dependent exocytotic event involving an increase in [Ca 21 ] i mediated primarily by T-type voltage-operated calcium channels (VOCCs). [28][29][30] A role for L-type VOCCs has also been proposed during induction of the AR.…”

Section: Signalling Events During Zp-mediated Acrosomal Exocytosismentioning

confidence: 99%

Acrosome reaction: relevance of zona pellucida glycoproteins

Gupta

Bhandari²

2010

Asian J Androl

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During mammalian fertilisation, the zona pellucida (ZP) matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction (AR) in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four (ZP1, ZP2, ZP3 and ZP4). ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans (in addition to ZP3), ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the G i -coupled receptor pathway, whereas ZP1-and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels (VOCCs), whereas ZP1-and ZP4-induced ARs involve both T-and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans (in addition to ZP3), ZP1 and ZP4 also participate in these stages of fertilisation. Keywords: acrosome reaction; fertilisation; oocyte; signalling pathways; spermatozoa; zona pellucida glycoproteins INTRODUCTION Mammalian fertilisation is a highly synchronized process that involves a complex series of interactions between the spermatozoon and the egg, culminating in their unison. The initial steps in fertilisation involves the binding of the spermatozoon to the zona pellucida (ZP) matrix surrounding the egg, followed by induction of the acrosome reaction (AR) in the zona-bound spermatozoon, a pre-requisite for penetration of the ZP matrix by the spermatozoon. The spermatozoon acrosome is a Golgi-derived organelle that forms a cap over the anterior two-thirds of its nucleus. The AR involves the fusion of the sperm membrane with the outer acrosomal membrane, resulting in release of the acrosomal contents and exposure of the inner acrosomal membrane on the anterior head of the spermatozoon. Various physiological agents, such as progesterone, serum albumin, follicular fluid, hormones (including biogenic amines), hydrolytic enzymes (particularly proteases), hyaluronic acid and ZP glycoproteins, have been implicated in the induction of the AR. [1][2][3] In the present paper, we review the role of the ZP matrix and its constituents in AR induction. Various downstream signalling pathways involved in the ZP glycoprotein-induced AR will also be discussed. On the basis of the current literature and studies from our group, the salient differences in the ZP glycoprotein-mediated induction of the AR in mouse versus human will be highlighted.

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A Perspective on the Control of Mammalian Fertilization by Egg-Activated Ion Channels in Sperm: A Tale of Two Channels1

Cited by 177 publications

References 45 publications

Sperm Na+, K+-ATPase and Ca2+-ATPase activity: A preliminary study of comparison of swim up and density gradient centrifugation methods for sperm preparation

Sperm Na+, K+-ATPase and Ca2+-ATPase activity: A preliminary study of comparison of swim up and density gradient centrifugation methods for sperm preparation

Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

Acrosome reaction: relevance of zona pellucida glycoproteins

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