Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

Zhang, Jinfu; Gui, Yan; Yuan, Tao; Bian, Cuidong; Guo, Lihe

doi:10.3109/19396360903030500

Cited by 7 publications

(14 citation statements)

References 18 publications

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“…However, there are no remarkable changes in the epididymis [15]. This can explain that the male reproduction is compromised in GAT1 transgenic mice [15,16]. In the current study, we found that GABA and GAT1 distributed differentially in mouse epididymis caput, corpus, and cauda.…”

Section: Discussionsupporting

confidence: 47%

“…Our previous studies demonstrate that there are abnormal histopathological presentations in testis and sperm from GAT1 transgenic mice. However, there are no remarkable changes in the epididymis [15]. This can explain that the male reproduction is compromised in GAT1 transgenic mice [15,16].…”

Section: Discussionmentioning

confidence: 80%

“…GABA (expressed in sperms, testes and epididymis) plays an important role in spermiotelcosis [13][14][15]. Our previous studies demonstrate that there are abnormal histopathological presentations in testis and sperm from GAT1 transgenic mice.…”

Section: Discussionmentioning

confidence: 99%

“…Thus GABA may be a physiological regulator of sperm function. GAT1 is also observed in testes, epididymis and sperms [13][14][15][16][17][18]. The function of GAT1 has been demonstrated, showing compromised reproductive capacity in the GAT1 transgenic mice.…”

Section: Introductionmentioning

confidence: 99%

“…The function of GAT1 has been demonstrated, showing compromised reproductive capacity in the GAT1 transgenic mice. The explanation of compromised reproduction of the GAT1 transgenic mice may be due to disturbed maturation of sperms, compared with wild type mice [15]. Therefore it is necessary to determine the distribution and/or correlation of GABA and GAT1 in epididymis, which may shed light in the role of these molecules in the maturation of sperm, which could be useful for understanding of male infertility with potential application in the management of such patients.…”

Section: Introductionmentioning

confidence: 99%

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Differential Distribution of GABA and GAT1 in Mouse Epididymis

Qi¹,

Hu²,

Guo³

et al. 2017

Ann Clin Lab Res

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The present study is to explore the relationship between GABA/GAT1 and mechanism of sperm maturation in epididymis. The distribution of GABA and GAT1 was observed under microscope in different magnifications. Immune labeled GABA and GAT1 at the different regions of epididymis was semi-quantified. Our data illustrated that GABA was significantly higher produced at caput of epididymis than that at corpus or cauda of epididymis. Consistently, the distribution of GAT1 showed similar patterns at the different regions of epididymis. The current data suggested that differential expression of GABA and its transport protein I (GAT1) at the different regions of epididymis may be related to maturation and subsequently the function of sperms. Such information may also contribute to our clinical practice in Andrology.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differential Distribution of GABA and GAT1 in Mouse Epididymis

Qi¹,

Hu²,

Guo³

et al. 2017

Ann Clin Lab Res

Self Cite

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Molecular genetic mechanisms of teratozoospermia

Chang

Jiang

Liu

et al. 2023

Zygote

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Summary In recent years, the incidence of teratospermia has been increasing, and it has become a very important factor leading to male infertility. The research on the molecular mechanism of teratospermia is also progressing rapidly. This article briefly summarizes the clinical incidence of teratozoospermia, and makes a retrospective summary of related studies reported in recent years. Specifically discussing the relationship between gene status and spermatozoa, the review aims to provide the basis for the genetic diagnosis and gene therapy of teratozoospermia.

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Differential protein expression of GABA A receptor alpha 1 subunit in rat spermatozoa associated with proteomic analysis in testis following methamphetamine administration

Nudmamud-Thanoi

Kaewman

Thongleart

et al. 2022

Preprint

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Methamphetamine (METH) can induce spermatogenesis impairment, testicular apoptosis, and abnormal sperm quality. It also promotes changes in the expression of receptors for sex hormones and neurotransmitters, including GABA receptors in the testis. Proteomic assessment focusing on proteins involved in the calcium signalling pathway in the testis can facilitate diagnostic factors contributing to testicular and sperm functions, especially those related to spermatogenesis and fertilisation. In this study, we proposed to determine the localization and differential expression of GABA A receptor alpha 1 subunit (GABA A-α1) in the spermatozoa of METH-administrated rats. The differential proteomic profile of the testis was also observed by focusing on proteins in the KEGG pathways belonging to the calcium signalling pathway. There were 212 differentially expressed proteins in the rat testis, based on the cut-off value of 1.2-fold change. Most of those proteins, 13 proteins, were classified in the calcium signalling pathway, including 4 down-regulated and 9 up-regulated proteins. An immunolocalization study of the GABA A-α1 receptor revealed its localization in the equatorial segment of the acrosome in the rat spermatozoa, a region involved in the acrosome reaction. An increase in GABA A-α1 receptor in rat spermatozoa was correlated with an increase in abnormal sperm motility and morphology after methamphetamine exposure. All our findings demonstrate that METH influences intracellular calcium homeostasis by acting through the calcium signalling pathway-associated proteins. Moreover, it might disrupt ion homeostasis in sperm through the GABA A-α1 receptor, triggering a change in intracellular calcium and chloride ions. These changes may cause abnormalities in spermatogenesis, testicular apoptosis, and sperm quality impairment.

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Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

Cited by 7 publications

References 18 publications

Differential Distribution of GABA and GAT1 in Mouse Epididymis

Differential Distribution of GABA and GAT1 in Mouse Epididymis

Molecular genetic mechanisms of teratozoospermia

Differential protein expression of GABA A receptor alpha 1 subunit in rat spermatozoa associated with proteomic analysis in testis following methamphetamine administration

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