A phage display system for the identification of novel Anisakis simplex antigens

López, Itziar Angulo; Pardo, Miguel Ángel

doi:10.1016/j.jim.2011.08.009

Cited by 18 publications

(10 citation statements)

References 9 publications

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“…This frames the necessity of improving diagnostic kits based on specific Anisakis antigens, e.g. purified natural or recombinant allergens [114–116] and has accelerated immunoscreening of protein-expressing cDNA libraries [117], phage display system [118], and mass spectrometry-based proteomics [54] to identify novel allergen candidates.…”

Section: Diagnosis and Es Productsmentioning

confidence: 99%

Excretory/secretory products of anisakid nematodes: biological and pathological roles

Mehrdana

Buchmann

2017

Acta Vet Scand

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Parasites from the family Anisakidae are widely distributed in marine fish populations worldwide and mainly nematodes of the three genera Anisakis, Pseudoterranova and Contracaecum have attracted attention due to their pathogenicity in humans. Their life cycles include invertebrates and fish as intermediate or transport hosts and mammals or birds as final hosts. Human consumption of raw or underprocessed seafood containing third stage larvae of anisakid parasites may elicit a gastrointestinal disease (anisakidosis) and allergic responses. Excretory and secretory (ES) compounds produced by the parasites are assumed to be key players in clinical manifestation of the disease in humans, but the molecules are likely to play a general biological role in invertebrates and lower vertebrates as well. ES products have several functions during infection, e.g. penetration of host tissues and evasion of host immune responses, but are at the same time known to elicit immune responses (including antibody production) both in fish and mammals. ES proteins from anisakid nematodes, in particular Anisakis simplex, are currently applied for diagnostic purposes but recent evidence suggests that they also may have a therapeutic potential in immune-related diseases.

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Section: Diagnosis and Es Productsmentioning

confidence: 99%

Excretory/secretory products of anisakid nematodes: biological and pathological roles

Mehrdana

Buchmann

2017

Acta Vet Scand

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“…Currently, phage display libraries are being developed as the phenotype is expressed directly on the virus, allowing for more expedient analysis. This technique has been applied to A. simplex, and thirty novel allergen candidates have been identified but have yet to be confirmed experimentally [96]. Also bioinformatic approaches are supporting these methods.…”

Section: Allergic Responses In Humans and Anisakid Allergensmentioning

confidence: 99%

Foodborne anisakiasis and allergy

Baird

Gasser

Jabbar

et al. 2014

Molecular and Cellular Probes

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Human anisakiasis, a disease caused by Anisakis spp. (Nematoda), is often associated with clinical signs that are similar to those associated with bacterial or viral gastroenteritis. With the globalisation of the seafood industry, the risk of humans acquiring anisakiasis in developed countries appears to be underestimated. The importance of this disease is not only in its initial manifestation, which can often become chronic if the immune response does not eliminate the worm, but, importantly, in its subsequent sensitisation of the human patient. This sensitisation to Anisakis-derived allergens can put the patient at risk of an allergic exacerbation upon secondary exposure. This article reviews some aspects of this food-borne disease and explains its link to chronic, allergic conditions in humans.

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“…Five copies of minor coat proteins p7, p9 and p3, p6 are found on head and tail parts respectively . Phage display technique is widely applied in order to identify specific short peptides with affinities to other peptides, proteins, solid materials, or cells . Fd phages are negatively charged at physiological conditions (pH 7.3).…”

Section: Introductionmentioning

confidence: 99%

Trivalent Cation Induced Bundle Formation of Filamentous fd Phages

Zirpel

Park

2015

Macromol. Biosci.

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Bacteriophages are filamentous polyelectrolyte viral rods infecting only bacteria. In this study, we investigate the bundle formation of fd phages with trivalent cations having different ionic radii (Al(3+) , La(3+) and Y(3+) ) at various phage and counterion concentrations, and at varying bundling times. Aggregated phage bundles were detected at relatively low trivalent counterion concentrations (1 mM). Although 10 mM and 100 mM Y(3+) and La(3+) treatments formed larger and more intertwined phage bundles, Al(3+) and Fe(3+) treatments lead to the formation of networking filaments. Energy dispersive X-ray spectroscopy (EDX) analyses confirmed the presence of C, N and O peaks on densely packed phage bundles. Immunofluorescence labelling and ELISA analyses with anti-p8 antibodies showed the presence of phage filaments after bundling.

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A phage display system for the identification of novel Anisakis simplex antigens

Cited by 18 publications

References 9 publications

Excretory/secretory products of anisakid nematodes: biological and pathological roles

Excretory/secretory products of anisakid nematodes: biological and pathological roles

Foodborne anisakiasis and allergy

Trivalent Cation Induced Bundle Formation of Filamentous fd Phages

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