A phage-displayed single chain variable fragment that interacts with hepatitis B core antigen: Library construction, selection and diagnosis

Abstract

The ScFv displayed on the surface of filamentous phage is an alternative choice for diagnosis of HBcAg in serum samples.

Use of an Enhanced Green Fluorescence Protein Linked to a Single Chain Fragment Variable Antibody to LocalizeBursaphelenchus xylophilusCellulase

“…The single chain fragment variable (scFv) is one smaller unit with the ability to conjugate with antigen, and it can penetrate cells and react against antigen more efficiently than normal antibody. Although scFvs have been successfully used in a large number of studies relating to cancer, viral infections, and aging, [6][7][8][9] they have not been used in the detection and localization of proteins. Enhanced green fluorescence protein (EGFP) has been widely used in signal detection in biology.…”