A photoelectrochemical cell using immobilized photosynthetic membranes

Carpentier, Robert; Lemieux, Sylvie; Mimeault, Murielle; Purcell, Marc; Goetze, D. Christopher

doi:10.1016/0302-4598(89)87055-2

Cited by 41 publications

(24 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is important to point out that this sensor is intended for early warning applications and as such is not capable of distinguishing between different explosives if they are present in unknown concentrations. Similar statement can be made concerning application of the PS IIbased biosensors for herbicide detection [7][8][9][10][11][12][13][14][15]. The present biosensor also may be employed in environmental protection and pharmaceutical applications for rapid screening of picric acid in water samples [18,19].…”

Section: Discussionmentioning

confidence: 53%

“…Both intact thylakoids [7,8,32,34,35] and PS II-enriched BBY particles [10,11,31] have been used in electrochemical cells and herbicide biosensors. At low temperature (4°C) and in the dark both BBY particles [31] and thylakoid membranes [36] can be stored for hundreds of hours.…”

Section: Resultsmentioning

confidence: 99%

“…The choice of the immobilization technique was based on its ability to preserve the biological sample in its active form for long periods of time. The procedure for bovine serum albumin (BSA)-glutaraldehyde matrix-based immobilization was similar to that described earlier [7,8,11,[31][32][33][34]. Briefly, a 10% solution of BSA in measuring buffer was mixed with equal amount of BBY particles.…”

Section: Biosensor Preparationmentioning

confidence: 99%

“…The idea for such sensor is inspired by recent progress in the development of inexpensive and sensitive herbicide biosensors based on photosynthetic reaction centers (RC). Since using PS II for herbicides detection was proposed more than 20 years ago [7] this subject has been explored extensively [8][9][10][11][12][13][14][15]. It is important to note that the chemical structures of nitric explosives are fairly similar to those of the herbicides detectable by these biosensors (Fig.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of explosive compounds using Photosystem II-based biosensor

Bhalla

Zhao

Zazubovich

2011

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

a b s t r a c tThe efficacy of a Photosystem II (PS II)-based biosensor for the detection of explosive compounds has been explored. The idea is based on the close similarities in the chemical structures of the widespread explosives and herbicides, with the latter known to inhibit functioning of the PS II by attaching to the binding site of the Q B mobile plastoquinone electron acceptor. The gold screen-printed electrodes (Au-SPE) functionalized with PS II-enriched particles were used for the detection of explosives in a droplet biosensor configuration. A crude preparation of PS II produced from spinach leaves, known as BBY particles, was employed to modify the Au-SPE working electrode employing BSA-glutaraldehyde-based immobilization procedure. Inhibition of the PS II functioning was detected by photo-electrochemical measurements in the presence of a mediator (either non-native quinone or ferricyanide). The biosensor was highly responsive to herbicides (as expected) as well as to picric acid, with limits of detection in the nanomolar range, but trace detection of trinitrotoluene (TNT) was not effective. The detection limit for picric acid was 25 nM as compared to $400 nM for TNT with duroquinone mediator. Low affinity of PS II to TNT has been corroborated by means of DCPIP assay; possible reasons for low affinity are discussed.

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Biosensor Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of explosive compounds using Photosystem II-based biosensor

Bhalla

Zhao

Zazubovich

2011

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Various attempts have been made to immobilize the isolated thylakoids onto different support matrices such as albuminglutaraldehyde cross-linked matrix, 4 multi-walled carbon nanotubes, 5 encapsulating the thylakoid membrane vesicles onto conductive nanofibers by electrospinning, 6 and vapor deposition of thin layer of silica onto the thylakoid layer. 7 PSI complexes have been immobilized using several different strategies such as gold nanoparticle (GNP) modified electrode, 8 functionalized nano-porous gold leaf electrodes, 9 PSI-GNP hybrid electrode modified with 3-mercapto-1-propanesulfonic acid, 10 and via self-assembly onto zinc oxide nanomaterials.…”

Section: Electricity Generationmentioning

confidence: 99%

Photosynthetic Energy Conversion: Recent Advances and Future Perspective

Sekar

Ramasamy

2015

Interface magazine

View full text Add to dashboard Cite

Photosynthesis is the most important natural process on earth, which transformed the once lifeless planet into a living world. While primitive photosynthetic bacteria such as purple sulfur bacteria and green sulfur bacteria carry out anoxygenic photosynthesis, producing elemental sulfur from hydrogen sulfide with the help of sunlight, cyanobacteria, algae and plants carry out oxygenic photosynthesis to convert water and carbon dioxide to sugars with the help of sunlight and release oxygen as a byproduct. The conversion of solar energy to chemical energy via photosynthesis with the release of oxygen has an evolutionary significance on life as we know it today. In fact, photosynthesis is the only natural process known on earth to form oxygen from water. Further, fossil fuels such as coal, petroleum and natural gas are formed from the remains of the dead plants by exposure to heat and pressure in the earth's crust over millions of years. With increasing energy crisis and environmental issues lately, now is the time to revisit photosynthesis in order to address these issues. In this context, a great deal of ongoing research is focused on utilizing photosynthetic energy conversion as a renewable, self-sustainable and environment friendly source of energy. When compared to the finite reserve of fossil fuels, sunlight, the energy source for photosynthesis, is abundant around the planet and is inexhaustible.The earth receives solar energy at the rate of about 120,000 TW, which far exceeds our current global demand of ~16 TW.1 However the only major technology available for solar energy conversion is photovoltaics (PV). PV devices such as solar panels generate electrical power by converting solar radiation into direct current electricity using semiconductors. Solar cells include first generation conventional wafer-based cells made up of crystalline silicon (Fig. 1a), second generation thin film solar cells (Fig. 1b)

show abstract