A physical map of chromosome 2 of Arabidopsis thaliana.

Abstract

A yeast artificial chromosome (YAC] physical map of chromosome 2 of Arabidopsis thaliana has been constructed by hybridization of 69 DNA markers and 61 YAC end probes to gridded arrays of YAC clones. Thirty-four YACs in four contigs define the chromosome. Complete closure of the map was not attained because some regions of the chromosome were repetitive or were not represented in the YAC library. Based on the sizes of the YACs and their coverage of the chromosome, the length of chromosome 2 is estimated to be at least 18 Mb. These data provide the means for immediately identifying the YACs containing a genetic locus mapped on Arabidopsis chromosome 2.

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana

“…Chromosome 2 is acrocentric and was originally estimated to be 13.5 Mb in length from the YAC-based physical map 12 . Sequencing was initiated using bacterial arti®cial chromosomes (BACs) that had been placed onto the physical map of chromosome 2 by hybridization to YACs and genetic markers.…”

Hexokinase as a sugar sensor in higher plants.

“…AtHXKI is located on chromosome 4 (Schmidt et al, 1995) and is flanked by mi232 and g8300. AtHXK2 is located on chromosome 2 (Zachgo et al, 1996) and is flanked by mil48 and mi238 ( Figure 3). Genomic DNA gel blot analysis using either AtHXKI or AtHXK2 as a probe revealed at least two AtHXK genes corresponding to the two isolated AtHXK cDNAs that were hybridized at the same level of stringency ( Figure 4).…”

Arabidopsis thaliana Centromere Regions: Genetic Map Positions and Repetitive DNA Structure

“…Aiding in this analysis is the availability of dense genetic maps that exist for each of A. thaliana's five chromosomes (e.g., Hauge et al 1993;Lister and Dean 1993). In addition, physical maps are being developed for all A. thaliana chromosomes in the form of overlapping cloned genomic fragments (Schmidt et al 1995;Zachgo et al 1996).Considering the generally advanced molecular characterization of the A. thaliana genome, the genomic organization and genetic location of centromeres in this species remain poorly characterized. The A. thaliana centromere regions are heterochromatic (Schweizer et al 1987) and contain tandem arrays of related repeats (exhibiting ജ80% similarity) that are ∼180 bp in length (Martinez-Zapater et al 1986;Simoens et al 1988;Maluszynska and Heslop-Harrison 1991), a genomic organization that resembles the ∼170-bp alphoid repeat arrays at primate centromeres (Willard 1990;Pluta et al 1995).…”

“…More recently, physical mapping projects have localized the centromeres on chromosomes 2 and 4 as gaps in the YAC contig maps that are bordered by genomic clones containing 180-bp repeats (Schmidt et al 1995;Zachgo et al 1996). We report here the mapping of all five A. thaliana centromere regions, identified by large polymorphic 180-bp repeat arrays, on the standard recombinant inbred genetic map.…”

“…Aiding in this analysis is the availability of dense genetic maps that exist for each of A. thaliana's five chromosomes (e.g., Hauge et al 1993;Lister and Dean 1993). In addition, physical maps are being developed for all A. thaliana chromosomes in the form of overlapping cloned genomic fragments (Schmidt et al 1995;Zachgo et al 1996).…”