A Phytochemical Study of Lignans in Whole Plants and Cell Suspension Cultures ofAnthriscus sylvestris

Abstract

In the roots of Anthriscus sylvestris 12 different lignans were detected. Arctigenin, dimethylmatairesinol, dimethylthujaplicatin, podophyllotoxin, 7-hydroxyyatein and 7-hydroxyanhydropodorhizol have not been previously reported to be present in A. sylvestris. In the cell suspension cultures, which were initiated for this study, trace amounts of deoxypodophyllotoxin could be detected. With these cell suspension cultures we carried out feeding experiments using deoxypodophyllotoxin, yatein and, anhydropodorhizol. Yatein had a toxic effect on the cell cultures and was, like anhydropodorhizol, not converted into any detectable product. Deoxypodophyllotoxin, in contrast, was converted into podophyllotoxin, yielding significantly higher concentration than measured in whole plants.

In vitro regeneration of wild chervil (Anthriscus sylvestris L.)

“…1 d ) and root explants from in vitro- grown seedlings. Several media were evaluated for callus induction in addition to the one reported previously for A. sylvestris (Koulman et al 2003) (Table 1). The best callus formation was obtained from roots with 75% induction using Gamborg’s B5 medium supplemented with 2.0 mg l −1 NAA and 0.2 mg l −1 Z (Table 1).…”

“…Calli of A. sylvestris did not produce DPT (Koulman et al 2003) and whole plants were required. Therefore, it is essential to achieve the differentiated form to restore the capability of the cell to produce DPT for metabolic engineering purposes.…”

“…After 2 mo, root, hypocotyl and leaf explants from young sterile in vitro- obtained seedlings were excised and cultured on Gamborg’s B5 media supplemented with either 2.0 mg l −1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg l −1 6-benzylaminopurine (BA) (Koulman et al 2003) or 2 mg l −1 α-naphthaleneacetic acid (NAA) and 0.2 or 1.0 mg l −1 zeatin riboside (Z) (Table 1). All media contained 40 g l −1 sucrose and 8 g l −1 agar (pH 5.7).…”

“…Plant material was dried at room temperature prior to grinding and extraction. The extraction method was as described by Koulman et al (2003), and the HPLC analysis was as described by Hendrawati et al (2011). …”

“…Using information on the initiation of A. sylvestris calli and suspension cultures (Koulman et al 2003), cells from callus tissue were used to establish a regeneration protocol for A. sylvestris . Dormancy problems from A. sylvestris seeds presented an additional concern (Baskin et al 2000).…”