A pilot study of pre-operative motor dysfunction from gliomas in the region of corticospinal tract: Evaluation with diffusion tensor imaging

Gao, Bo; Shen, Xudong; Shiroishi, Mark S.; Pang, Mingfan; Li, Zhiqian; Yu, Benxia; Shen, Guiquan

doi:10.1371/journal.pone.0182795

Cited by 15 publications

(22 citation statements)

References 36 publications

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“…Binding of IgM and IgG was assessed as the relative geometric mean (rGM), calculated by dividing the geometric mean fluorescence for each sample by that of the negative control, as previously described. 7 , 13 …”

Section: Methodsmentioning

confidence: 99%

“… 4 – 6 However, like pigs, Old World NHPs express N-glycolylneuraminic acid (Neu5Gc), and therefore do not develop natural anti-Neu5Gc antibodies. 7 If the CMP-N-acetylneuraminic acid hydroxylase (CMAH) gene is knocked out (resulting in deletion of expression of Neu5Gc), as in TKO pigs, this appears to expose 1 or more new xenoantigens on the pig cells (the so-called “fourth xenoantigen”). 8 In contrast, if CMAH is not knocked out (eg, in α1,3-galactosyltransferase gene-knockout [GTKO] and GTKO/β-1,4 N-acetylgalactosaminyltransferase gene-knockout [β4GalNT2KO] pigs), the pig appears not to express the “fourth antigen” (or expresses it at a lower level).…”

Section: Introductionmentioning

confidence: 99%

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Evidence for GTKO/β4GalNT2KO Pigs as the Preferred Organ-source for Old World Nonhuman Primates as a Preclinical Model of Xenotransplantation

Cui

Yamamoto

Raza

et al. 2020

Transplantation Direct

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Background. Triple-knockout (TKO) pigs (in which expression of the 3 known pig carbohydrate xenoantigens has been deleted) are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural antibodies against TKO pig cells. However, old world monkeys, for example, baboons, have natural antibodies directed to TKO cells (to a “fourth” xenoantigen that is exposed after TKO). Methods. We measured (1) anti-pig IgM/IgG binding, and (2) complement-dependent cytotoxicity (CDC), by flow cytometry to α1,3-galactosyltransfearse gene-knockout (GTKO), GTKO/β4GalNT2KO (that do not express the “fourth” xenoantigen), and TKO pig peripheral blood mononuclear cells (PBMCs) using 72 baboon sera (30 specific pathogen-free [SPF], and 42 non-SPF baboons). Results. Mean IgM antibody binding to GTKO/β4GalNT2KO pig PBMCs was significantly lower than to GTKO or TKO pig PBMCs (P < 0.01). Mean IgG antibody binding to GTKO/β4GalNT2KO pig PBMCs was significantly lower than to TKO PBMCs (P < 0.01). Mean CDC of GTKO/β4GalNT2KO pig PBMCs was significantly lower than of GTKO or TKO pig PBMCs (P < 0.01). SPF baboon serum IgM and IgG binding to, and CDC of, GTKO/β4GalNT2KO or TKO PBMCs were significantly lower than non-SPF baboon sera (P < 0.01). Conclusions. Although TKO pigs form the basis for proposed clinical trials of xenotransplantation, it is difficult to identify baboons with a low or negative CDC to TKO pigs. For pig-to-baboon organ transplantation, the use of GTKO/β4GalNT2KO pigs would be preferable. The use of SPF baboons as recipients might be a minor advantage.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence for GTKO/β4GalNT2KO Pigs as the Preferred Organ-source for Old World Nonhuman Primates as a Preclinical Model of Xenotransplantation

Cui

Yamamoto

Raza

et al. 2020

Transplantation Direct

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“…In this study, the white matter tracts in 17 glioma-bearing rats were classified according to the criteria of displacement, infiltration and disruption (Gao et al, 2017; Witwer et al, 2002) as follows: (1) grade 1: displaced and edematous if the tracts displayed a normal anisotropy signal relative to the corresponding tracts in the contralateral hemisphere, displacement of the fibers by the tumor and some peritumoral edema delineated by the DTT imaging (Fig. S1).…”

Section: Methodsmentioning

confidence: 99%

“…Rosenstock et al (2017) showed that the FA value were lower and the ADC value were higher in peritumoral areas, which indicated that the FA and ADC value were affected at the peritumoral level. Additionally, tumor cell proliferation and invasion could result in a disruption of CST, and the degree of injury may reflect its biological behavior (Gao et al, 2017; Painter & Hillen, 2013).…”

Section: Introductionmentioning

confidence: 99%

The value of diffusion tensor tractography delineating corticospinal tract in glioma in rat: validation via correlation histology

Jia

Su²,

et al. 2019

PeerJ

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BackgroundAn assessment of the degree of white matter tract injury is important in neurosurgical planning for patients with gliomas. The main objective of this study was to assess the injury grade of the corticospinal tract (CST) in rats with glioma using diffusion tensor imaging (DTI).MethodsA total 17 rats underwent 7.0T MRI on day 10 after tumor implantation. The apparent diffusion coefficient (ADC) and fractional anisotropy (FA) were acquired in the tumor, peritumoral and contralateral areas, and the ADC ratio (ipsilateral ADC/contralateral ADC) and rFA (relative FA = ipsilateral FA/contralateral FA) in the peritumoral areas were measured. The CST injury was divided into three grades and delineated by diffusion tensor tractography reconstruction imaging. The fiber density index (FDi) of the ipsilateral and contralateral CST and rFDi (relative FDi = ipsilateral FDi/contralateral FDi) in the peritumoral areas were measured. After the mice were sacrificed, the invasion of glioma cells and fraction of proliferating cells were observed by hematoxylin-eosin and Ki67 staining in the tumor and peritumoral areas. The correlations among the pathology results, CST injury grade and DTI parameter values were calculated using a Spearman correlation analysis. One-way analysis of variance was performed to compare the different CST injury grade by the rFA, rFDi and ADC ratio values.ResultsThe tumor cells and proliferation index were positively correlated with the CST injury grade (r = 0.8857, 0.9233, P < 0.001). A negative correlation was demonstrated between the tumor cells and the rFA and rFDi values in the peritumoral areas (r = −0.8571, −0.5588), and the proliferation index was negatively correlated with the rFA and rFDi values (r = −0.8571, −0.5588), while the ADC ratio was not correlated with the tumor cells or proliferation index. The rFA values between the CST injury grades (1 and 3, 2 and 3) and the rFDi values in grades 1 and 3 significantly differed (P < 0.05).ConclusionsDiffusion tensor imaging may be used to quantify the injury degrees of CST involving brain glioma in rats. Our data suggest that these quantitative parameters may be used to enhance the efficiency of delineating the relationship between fiber tracts and malignant tumor.

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“…Various ROIs can be used to track CST, resulting in high variability rate. Weiss et al showed that an ROI set at the anterior inferior pontine region yielded better tracking results compared to the ROI set at the internal capsule [28][29][30]. Furthermore, CST was reconstructed from neural bundles passing through by cerebral peduncle, posterior limb of internal capsule and corona radiata in a patient after stroke [31].…”

Section: Corticospinal Tractmentioning

confidence: 99%

The usefulness and limitations of diffusion tensor imaging – a review study

Kierońska¹,

Słoniewski²

2020

Eur J Trans Clin Med

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Diffusion tensor tractography (DTI) has been used for planning of a brain pathology surgeries. Knowledge about the distances between neural tracts and brain tumours is believed to increase the patient safety and implies the extent of resection. The aim of the study was to demonstrate the contemporary possibilities and the clinical usefulness of DTI. Following the explanation of the technical basics of DTI, we presented the drawbacks and limitations of this visualisation technique. The most commonly outlined tracts are corticospinal tract (CST), arcuate fasciculus (AF) and frontal aslant tract (FAT). Tumour located in frontal, parietal or temporal lobe can affect the course of the CST. There are two basic possibilities to visualise CST: deterministic and probabilistic. The usefulness of DTI seems limited in imaging the neoplasms of either frontal or temporal region causing aphasia, which infiltrate the AF or the FAT. This limitation is probably related to divergent and patient-specific location of functional speech areas. Acquisition disturbances, ill-defined mathematical algorithms, surgery-related brain shift and defining wrong non-functional brain area are the sources of DTI inaccuracy, which is limiting its clinical application.

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A pilot study of pre-operative motor dysfunction from gliomas in the region of corticospinal tract: Evaluation with diffusion tensor imaging

Abstract: Background and purpose

Cited by 15 publications

References 36 publications

Evidence for GTKO/β4GalNT2KO Pigs as the Preferred Organ-source for Old World Nonhuman Primates as a Preclinical Model of Xenotransplantation

Evidence for GTKO/β4GalNT2KO Pigs as the Preferred Organ-source for Old World Nonhuman Primates as a Preclinical Model of Xenotransplantation

The value of diffusion tensor tractography delineating corticospinal tract in glioma in rat: validation via correlation histology

The usefulness and limitations of diffusion tensor imaging – a review study

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