A pilot study of using multiphoton microscopy to diagnose gastric cancer

Yan, Jun; Chen, Gang; Chen, Jianxin; Liu, Nenrong; Zhuo, Shuangmu; Yu, Hui; Ying, Mingang

doi:10.1007/s00464-010-1409-z

Cited by 38 publications

(29 citation statements)

References 25 publications

(25 reference statements)

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“…119 found they could distinguish cancer from control gastric tissue with simple qualitative comparisons of observed TPM SHG and TPEF features, which also correlated well with common identifying characteristics on the equivalent stained histopathology slides. Similar observations were made using TPM of intrinsic signals alone to distinguish between normal, nodular goiter, and papillary cancerous thyroid tissues.…”

Section: Utilization Of Tpm In Clinical and Translational Cancer Resesupporting

confidence: 52%

Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

2012

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Application of two-photon microscopy (TPM) to translational and clinical cancer research has burgeoned over the last several years, as several avenues of pre-clinical research have come to fruition. In this review, we focus on two forms of TPM—two-photon excitation fluorescence microscopy, and second harmonic generation microscopy—as they have been used for investigating cancer pathology in ex vivo and in vivo human tissue. We begin with discussion of two-photon theory and instrumentation particularly as applicable to cancer research, followed by an overview of some of the relevant cancer research literature in areas that include two-photon imaging of human tissue biopsies, human skin in vivo, and the rapidly developing technology of two-photon microendoscopy. We believe these and other evolving two-photon methodologies will continue to help translate cancer research from the bench to the bedside, and ultimately bring minimally invasive methods for cancer diagnosis and treatment to therapeutic reality.

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Section: Utilization Of Tpm In Clinical and Translational Cancer Resesupporting

confidence: 52%

Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

2012

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“…[65] demonstrated excellent comparability between labelfree NLOI and HE staining in differentiating between various normal and neoplastic tissues of the gastro-intestinal tract. Later on, Yan et al was able to provide cyto-structural details that could identify normal and gastric cancer samples [66]. Similarly good correlation was demonstrated between ex vivo label-free NLOI and HE stained slides in various other organs such as oral cavity [14], lungs [67][68][69], breast [70], ovaries [29,71,72], prostate [10], colon [73,74], rectum (as shown in Fig.…”

Section: Bio-safety Evaluationmentioning

confidence: 80%

Advances and challenges in label-free nonlinear optical imaging using two-photon excitation fluorescence and second harmonic generation for cancer research

Thomas

Voskuilen

Gerritsen

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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“…Continued development of MPM technology, new infrared dyes (long wavelength penetrates deeper due to less absorption and scattering), ultrasensitive hybrid detectors, and microscope objectives designed specifically for MPM may soon allow imaging deeper in the living, intact renal cortex and perhaps in the renal medulla. In addition to its research use in animals, MPM continues to be applied in newer and newer areas of human diagnostics, such as for the evaluation of skin diseases (19) and in endoscopy (63). Thus MPM offers unmatched versatility for biological and medical imaging studies of the living organism.…”

Section: Future Directionsmentioning

confidence: 99%

The first decade of using multiphoton microscopy for high-power kidney imaging

Peti‐Peterdi

Burford

Hackl

2012

American Journal of Physiology-Renal Physiology

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Peti-Peterdi J, Burford JL, Hackl MJ. The first decade of using multiphoton microscopy for high-power kidney imaging. Am J Physiol Renal Physiol 302: F227-F233, 2012. First published October 26, 2011 doi:10.1152/ajprenal.00561.2011In this review, we highlight the major scientific breakthroughs in kidney research achieved using multiphoton microscopy (MPM) and summarize the milestones in the technological development of kidney MPM during the past 10 years. Since more and more renal laboratories invest in MPM worldwide, we discuss future directions and provide practical, useful tips and examples for the application of this still-emerging optical sectioning technology. Advantages of using MPM in various kidney preparations that range from freshly dissected individual glomeruli or the whole kidney in vitro to MPM of the intact mouse and rat kidney in vivo are reviewed. Potential combinations of MPM with micromanipulation techniques including microperfusion and micropuncture are also included. However, we emphasize the most advanced and complex, quantitative in vivo imaging applications as the ultimate use of MPM since the true mandate of this technology is to look inside intact organs in live animals and humans. intravital imaging; juxtaglomerular apparatus; podocyte; two-photon microscopy IT HAS BEEN MORE THAN 10 YEARS since two-or three-photon excitation, collectively called multiphoton microscopy (MPM), was first applied to the study of the living kidney tissue by the Peti-Peterdi-Bell (37, 38) and Dunn-Molitoris groups (6, 42) that pioneered several applications of this powerful new imaging technology. Over the past decade, MPM has provided stunning images and real-time movies of the structure and function of the intact kidney in unparalleled spatial and temporal resolution. Because of the ability of this noninvasive imaging approach to directly visualize dynamic intrarenal processes in vivo and in near real-time without causing tissue damage, MPM has revolutionized renal physiology research and served as the perfect complement to more traditional biological and histological techniques. MPM also opened the door to studying otherwise inaccessible cell types and complex tissue structures like the glomerular podocyte (30, 39) and the juxtaglomerular apparatus (JGA) (37) in their intact environment. Applications of MPM helped to change several paradigms in renal (patho)physiology. Imaging data have been incorporated into a number of textbooks on kidney function (3,8,41), and MPM videos (30,37,39,40) have been used as visual aid and teaching material in graduate and medical student classrooms worldwide.MPM offers a state-of-the-art imaging technique superior for deep optical sectioning of living tissues. The higher resolution and minimal phototoxicity of this method permit longer time periods of continuous tissue scanning with uses in near realtime imaging of intact organs in vivo. MPM has applications far beyond the generation of superior images: dynamic intracellular processes as well as more complex physiological ...

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A pilot study of using multiphoton microscopy to diagnose gastric cancer

Abstract: It is feasible to use MPM to diagnose gastric cancer by "optical biopsy." With miniaturization and integration of endoscopy, MPM has the potential to provide real-time histological diagnosis without invasive biopsy for gastric cancer in the future.

Cited by 38 publications

References 25 publications

Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

Advances and challenges in label-free nonlinear optical imaging using two-photon excitation fluorescence and second harmonic generation for cancer research

The first decade of using multiphoton microscopy for high-power kidney imaging

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