Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

Perry, Seth W.; Burke, Ryan M.; Brown, Edward B.

doi:10.1007/s10439-012-0512-9

Cited by 171 publications

(141 citation statements)

References 125 publications

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“…This is further corroborated by Ait El Madani et al's results showing an increase in two-photon excited fluorescence signal intensity with increasing melanin density in human skin. 53 The τ 1 and τ 2 fluorescence lifetime values we obtained from the synthetic melanin solutions are close to the first two lifetimes reported by Teuchner et al 54,55 and Nighswander-Rempel et al 32 for synthetic melanin dissolved in dimethyl sulfoxide (DMSO) and water, respectively (Table 1). Unlike those authors, however, we did not observe a third lifetime component as all the fluorescence decay curves [e.g., Fig.…”

Section: Synthetic Melanin Solutionssupporting

confidence: 87%

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Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentationin vivo

et al. 2013

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Abstract. There is a growing body of literature showing the usefulness of multiphoton tomography (MPT) and fluorescence lifetime imaging for in situ characterization of skin constituents and the ensuing development of noninvasive diagnostic tools against skin diseases. Melanin and pigmentation-associated skin cancers constitute some of the major applications. We show that MPT and fluorescence lifetime imaging can be used to measure changes in cutaneous melanin concentration and that these can be related to the visible skin color. Melanin in the skin of African, Indian, Caucasian, and Asian volunteers is detected on the basis of its emission wavelength and fluorescence lifetimes in solution and in a melanocyte-keratinocyte cell culture. Fluorescence intensity is used to characterize the melanin content and distribution as a function of skin type and depth into the skin (stratum granulosum and stratum basale). The measured fluorescence intensities in given skin types agree with melanin amounts reported by others using biopsies. Our results suggest that spatial distribution of melanin in skin can be studied using MPT and fluorescence lifetime imaging, but further studies are needed to ascertain that the method can resolve melanin amount in smaller depth intervals.

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Section: Synthetic Melanin Solutionssupporting

confidence: 87%

“…Tables 2 and 3 53 We have used MPT and FLIM to investigate the relationship between visible skin color and melanin content and distribution in the skin of volunteers. We show here that in vivo changes in skin melanin content and distribution, as evidenced by measured …”

Section: Introductionmentioning

confidence: 99%

Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentationin vivo

et al. 2013

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“…Fig. 1 presents hematoxylin/eosin (H&E)-stained biopsy slices of breast cancer imaged by second harmonic generation (SHG) under a two-photon confocal microscopy (A1R MP; Nikon) (detailed information provided in SI Appendix, SI Text) (6,14,15). Fig.…”

mentioning

confidence: 99%

Oriented collagen fibers direct tumor cell intravasation

Han

Chen

Yuan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

320

248

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In this work, we constructed a Collagen I-Matrigel composite extracellular matrix (ECM). The composite ECM was used to determine the influence of the local collagen fiber orientation on the collective intravasation ability of tumor cells. We found that the local fiber alignment enhanced cell-ECM interactions. Specifically, metastatic MDA-MB-231 breast cancer cells followed the local fiber alignment direction during the intravasation into rigid Matrigel (∼10 mg/mL protein concentration).M etastasis is a lethal milestone in cancer: Cells escape from the confinement of primary tumor sites (intravasation), invade tissues as well as the lymphatic and vascular systems, and finally colonize (extravasation) distant sites. It has been estimated that less than 1% of tumor cells undergo this process, but metastasis contributes to more than 90% of cancerrelated deaths (1, 2). Metastasis involves both genetic and epigenetic alternation of tumor cells, as well as external biochemical and biophysical microenvironments (3-5). Pathology studies suggest that metastatic tumor cells exhibit highly branched morphologies and distinct aligned registration with aligned extracellular matrix (ECM) during metastatic tumor progression (4, 5).We address three important questions concerning metastasis. (i) Can we build in vitro complex ECM structures with heterogeneously oriented collagen fibers and basement membrane components to mimic the cancer cell intravasation process? (ii) How does aligned collagen influence cell intravasation into/ through the basement membrane before entering vessels? (iii) After cell detachment from the primary tumor site, how does a heterogeneous ECM with a varying degree of local fiber alignment influence cell intravasation and subsequent penetration into the basement membrane during their intravasation process? The major obstacle to addressing these questions is the difficulty in constructing both an in vitro 3D microenvironment to mimic the above process and flexible controls of the environmental parameters, such as fiber orientations in a complex collagen/Matrigel composite, nutrition, oxygen, drug concentrations, etc.In breast cancer metastasis, cancer cells are believed to reorganize and progress through the interstitial ECM matrix, break through the basement membrane, and enter blood vessels or lymphatic capillaries (6-10). Fig. 1C presents a schematic illustration of the intravasation process in metastasis. Tumor-associated collagen signatures (TACS), basically environmentally elevated collagen density and collagen fiber reorganization, are used to stage mammary carcinoma tumor progression levels (6, 11-13). Fig. 1 presents hematoxylin/eosin (H&E)-stained biopsy slices of breast cancer imaged by second harmonic generation (SHG) under a two-photon confocal microscopy (A1R MP; Nikon) (detailed information provided in SI Appendix, SI Text) (6, 14, 15). Fig. 1 A, 1-3 shows the stained human invasive ductal carcinoma tumor at grade I. In the enlarged figures (Fig. 1 A, 2 and 3), the cells have well-defined bord...

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“…However, despite these advantages, it is only over the last few years that SHG imaging of collagen structures in tissue has begun to be adopted more widely. A recent review was published on the development and burgeoning use of SHG imaging in the clinic to investigate collagen remodelling in cancer in ex vivo and in vivo human tissue [28]; changes in collagen structure during fibrosis are also being monitored; ex vivo in humans, in vivo in animals, by SHG imaging [29][30][31]. In addition SHG imaging is now playing an important role in non-invasive imaging of collagen structure, formation and remodelling in tissue engineering and regenerative medicine, as reviewed recently by Vielreciher et al [32].…”

Section: Discussionmentioning

confidence: 99%

Measurement of Friction-induced Changes in Pig Aorta Fibre Organization by Non-invasive Imaging as a Model for Detecting the Tissue Response to Endovascular Catheters

et al. 2017

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Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

Cited by 171 publications

References 125 publications

Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentationin vivo

Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentationin vivo

Oriented collagen fibers direct tumor cell intravasation

Measurement of Friction-induced Changes in Pig Aorta Fibre Organization by Non-invasive Imaging as a Model for Detecting the Tissue Response to Endovascular Catheters

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