2022
DOI: 10.1101/2022.03.10.22272226
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

A Pilot Validation Study Comparing FIBI, a Slide-Free Imaging Method, with Standard FFPE H&E Tissue Section Histology for Primary Surgical Pathology Diagnosis

Abstract: Introduction: Digital pathology whole slide images (WSI) have been recently approved by the FDA for primary diagnosis in clinical surgical pathology practices. These WSI are generated by digitally scanning standard formalin-fixed and paraffin-embedded (FFPE) H&E-stained tissue sections mounted on glass microscope slides. Novel imaging methods are being developed that can capture the surface of tissue without requiring prior fixation, paraffin embedding, or tissue sectioning. One of these methods, FIBI (Flu… Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
9
0

Year Published

2022
2022
2023
2023

Publication Types

Select...
4

Relationship

2
2

Authors

Journals

citations
Cited by 4 publications
(9 citation statements)
references
References 24 publications
0
9
0
Order By: Relevance
“…The tissue biopsies included in the preliminary study originated from the bladder, larynx, endocrine, breast, reproductive, gastrointestinal, and soft tissues with diagnoses including normal, hyperplasia, benign neoplasms, cancers, and inflammatory lesions. 6 Future studies are needed to evaluate and validate the utility of FIBI for neoplastic and noninflammatory lesions.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…The tissue biopsies included in the preliminary study originated from the bladder, larynx, endocrine, breast, reproductive, gastrointestinal, and soft tissues with diagnoses including normal, hyperplasia, benign neoplasms, cancers, and inflammatory lesions. 6 Future studies are needed to evaluate and validate the utility of FIBI for neoplastic and noninflammatory lesions.…”
Section: Discussionmentioning
confidence: 99%
“…A validation study evaluating the diagnostic concordance of FIBI images compared with conventional H&E assessment on a wide array of human tissues including breast, bladder, and gastrointestinal tissue, with diagnosis varying from normal/benign, cancer, and noncancerous origins, reported a concordance of 97.0%. 6 This observational, proof-of-concept study aimed to evaluate the diagnostic utility of FIBI for the pathological assessment of formalin-fixed paraffin-embedded (FFPE) intestinal tissue biopsies from cats with FCE. We hypothesized that FIBI is an efficient, affordable, and reliable diagnostic tool for the diagnosis and differentiation of FCE.…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…However, these technologies are expensive and require complex instrumentation, which has precluded widespread adoption of slide‐free microscopy in pathology. In contrast, we present a novel slide‐free microscopy technique that is inexpensive and relies on simple optical instrumentation 11 . Fluorescence imitating brightfield imaging (FIBI) uses standard microscope optics and a color camera to detect signals from tissue specimens stained with standard H&E. Even though an epifluorescence light path is used, the resulting FIBI images already resemble traditional H&E‐stained slides imaged via brightfield; and deep learning mode‐matching can be used to further increase the resemblance.…”
Section: Introductionmentioning
confidence: 99%
“…In contrast, we present a novel slide-free microscopy technique that is inexpensive and relies on simple optical instrumentation. 11 Fluorescence imitating brightfield imaging (FIBI) uses standard microscope optics and a color camera to detect signals from tissue specimens stained with standard H&E. Even though an epifluorescence light path is used, the resulting FIBI images already resemble traditional H&E-stained slides imaged via brightfield; and deep learning mode-matching can be used to further increase the resemblance. Our study compares FIBI microscopy with conventional H&E for dermatopathology specimens.…”
mentioning
confidence: 99%