A Plant Steroid, Diosgenin, a New Megakaryocytic Differentiation Inducer of Hel Cells

Beneytout, Jean-Louis; Nappez, C.; Leboutet, M. J.; Malinvaud, G

doi:10.1006/bbrc.1995.1201

Cited by 41 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As SdFFF only takes advantages of biophysical cell properties (size, density, shape, rigidity), it could be described as a tagless method [4,5,7,12,[15][16][17][18][19]. Since the work of Caldwell et al [7] on mammalian cells, FFF, SdFFF and related technologies have been used in many biological fields such as hematology, microorganism analysis, erythroleukemia cell line [47]. Moreover, diosgenin has been shown to exert antiproliferative and pro-apoptotic actions on rheumatoid arthritis synoviocytes [48] or on cancer cell lines [15,18,[49][50][51][52][53].…”

Section: Introductionmentioning

confidence: 99%

Study of diosgenin-induced apoptosis kinetics in K562 cells by Sedimentation Field Flow Fractionation

Bertrand

Liagre

Bégaud-Grimaud

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Study of diosgenin-induced apoptosis kinetics in K562 cells by Sedimentation Field Flow Fractionation

Bertrand

Liagre

Bégaud-Grimaud

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

“…8,9) Recent studies have shown that they have cytotoxic properties against several strains of human cancer cells. [10][11][12][13][14] Dioscin [15][16][17] and trillin 18) are the best known diosgenyl saponins with these properties (Fig. 1).…”

mentioning

confidence: 99%

The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines

Liu

Wang

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Diosgenyl saponins are the most abundant steroid saponins, and exert a large variety of biological functions. In a previous report, we showed that dioscin was able to induce cytotoxicity and apoptosis in human myeloblast leukemia HL-60 cells. This study further investigated the action mechanisms underlying this effect. The activation of caspase-9 and -3, but not caspase-8, together with the down-regulation of anti-apoptotic Bcl-2 protein, demonstrated that the apoptotic signaling triggered by dioscin was mediated through the intrinsic mitochondriadependent pathway. We also investigated its anti-proliferative effect on human chronic myelogenous leukemia K562 cells. Flow cytometry analysis showed that dioscin treatment induced the accumulation of cells in the G 2 /M phase. Cytomorphology with DAPI and Wright-Giemsa staining demonstrated the enlargement of cell volume and multinucleation in the treated cells. Subsequent apoptosis was delineated with phosphatidylserine externalization and DNA hypodiploidy. Trillin was one of the hydrolysates of dioscin. We demonstrated that it could induce multinucleation in HL-60, K562 and human promyelocytic leukemia NB 4 cells, suggesting its extensive mitotic-arresting effects. As the diosgenyl sapogenin, diosgenin was also shown to be able to induce multinucleation and apoptosis in K562 cells in a similar manner to dioscin. These findings suggest that diosgenyl saponins have the properties to induce mitotic arrest and apoptosis, suggesting that they may be a new kind of antimitotic agent.

show abstract

“…A recent study showed the involvement of the Sonic Hedgehog pathway in diosgenin-induced megakaryocytic differentiation in HEL cells (Ghezali et al, 2014). Further studies are required to understand the exact mechanism by which diosgenin induces megakaryocytic differentiation and its use in treating hematological disorders (Beneytout et al, 1995).…”

Section: Diosgeninmentioning

confidence: 98%

“…In human erythroleukemia (HEL) cells, diosgenin was found to induce morphological and biochemical changes, which is characteristic of megakaryocyte cells (Beneytout et al, 1995). After treating with diosgenin the HEL cells showed changes in its morphology, ploidy and also an increase in the cytoplasmic and nuclear complexity.…”

Section: Diosgeninmentioning

confidence: 99%

Erythropoietin and thrombopoietin mimetics: Natural alternatives to erythrocyte and platelet disorders

Gutti

Pasupuleti

Sahu

et al. 2016

Critical Reviews in Oncology/Hematology

View full text Add to dashboard Cite

A Plant Steroid, Diosgenin, a New Megakaryocytic Differentiation Inducer of Hel Cells

Cited by 41 publications

References 0 publications

Study of diosgenin-induced apoptosis kinetics in K562 cells by Sedimentation Field Flow Fractionation

Study of diosgenin-induced apoptosis kinetics in K562 cells by Sedimentation Field Flow Fractionation

The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines

Erythropoietin and thrombopoietin mimetics: Natural alternatives to erythrocyte and platelet disorders

Contact Info

Product

Resources

About