A plant virus satellite RNA directly accelerates wing formation in its insect vector for spread

Jayasinghe, Wikum Harshana; Kim, Hangil; Nakada, Yusuke; Masuta, Chikara

doi:10.1038/s41467-021-27330-4

Cited by 31 publications

(31 citation statements)

References 36 publications

(38 reference statements)

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“…This was seen more consistently on plants infected with LS‐CMV (subgroup II) (Tungadi et al, 2020). Similar positive effects on aphid survival and fecundity were reported for plants of the tobacco line BY4 (Jayasinghe et al, 2021) infected with another subgroup I CMV strain, CMV‐O (Jayasinghe et al, 2022).…”

Section: Figuresupporting

confidence: 69%

Induction of aphid resistance in tobacco by the cucumber mosaic virus CMV∆2b mutant is jasmonate‐dependent

Arinaitwe

Tungadi

Pate

et al. 2023

Molecular Plant Pathology

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Cucumber mosaic virus (CMV) is vectored by aphids, including Myzus persicae. Tobacco (Nicotiana tabacum ‘Xanthi’) plants infected with a mutant of the Fny strain of CMV (Fny‐CMVΔ2b, which cannot express the CMV 2b protein) exhibit strong resistance against M. persicae, which is manifested by decreased survival and reproduction of aphids confined on the plants. Previously, we found that the Fny‐CMV 1a replication protein elicits aphid resistance in plants infected with Fny‐CMVΔ2b, whereas in plants infected with wild‐type Fny‐CMV this is counteracted by the CMV 2b protein, a counterdefence protein that, among other things, inhibits jasmonic acid (JA)‐dependent immune signalling. We noted that in nontransformed cv. Petit Havana SR1 tobacco plants aphid resistance was not induced by Fny‐CMVΔ2b, suggesting that not all tobacco varieties possess the factor(s) with which the 1a protein interacts. To determine if 1a protein‐induced aphid resistance is JA‐dependent in Xanthi tobacco, transgenic plants were made that expressed an RNA silencing construct to diminish expression of the JA co‐receptor CORONATINE‐INSENSITIVE 1. Fny‐CMVΔ2b did not induce resistance to M. persicae in these transgenic plants. Thus, aphid resistance induction by the 1a protein requires JA‐dependent defensive signalling, which is countered by the CMV 2b protein.

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Section: Figuresupporting

confidence: 69%

Induction of aphid resistance in tobacco by the cucumber mosaic virus CMV∆2b mutant is jasmonate‐dependent

Arinaitwe

Tungadi

Pate

et al. 2023

Molecular Plant Pathology

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“…Interestingly, two of them, forkhead box protein O (Foxo) and ATP-binding cassette sub-family G member 4-like (ABCG4) could be involved in aphid wing formation (Shang et al, 2020;Grantham et al, 2020). Induction of wings could considerably increase virus propagation by aphids, especially over long distances, as recently shown for CMV transmission (Jayasinghe et al, 2021). In this specific case, the wing formation was attributed to a virus satellite coinfecting the plant.…”

Section: Virus-specific Aphid Degs On Both Host-plants 2a Tuyv-specif...mentioning

confidence: 80%

Transcriptome responses of the aphid vector Myzus persicae are shaped by identities of the host plant and the virus

Chesnais¹,

Golyaev²,

Velt³

et al. 2022

Peer Community Journal

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Background: Numerous studies have documented modifications in vector orientation behavior, settling and feeding behavior, and/or fecundity and survival due to virus infection in host plants. These alterations are often expected to enhance virus transmission, which has led to the hypothesis that such effects are vector manipulations by the virus. However, until now, the gene expression changes correlating with these effects and indicative of modified vector pathways and mechanisms are mostly unknown. Results: Transcriptome profiling of Myzus persicae aphids feeding on turnip yellows virus (TuYV) and cauliflower mosaic virus (CaMV) infected Arabidopsis thaliana and Camelina sativa revealed a substantial proportion of commonly deregulated genes, amongst them many with general functions in plant-virus-aphid interactions. We identified also aphid genes specifically deregulated by CaMV or TuYV infection, which might be related to the viral transmission mode. Furthermore, we observed strong host-specific differences in the gene expression patterns with plant virus infection causing more deregulations of aphid genes on A. thaliana than on C. sativa, likely related to the differences in susceptibility of the plant hosts to these viruses. Finally, stress-related aphid genes were downregulated in M. persicae on both infected plants, regardless of the virus. Conclusions: TuYV, relying on the circulative persistent mode of transmission, tended to affect developmental genes. This could increase the proportion of alate aphids, but also affect their locomotion, neuronal activity, and lifespan. CaMV, using the non-circulative nonpersistent mode of transmission, had a strong impact on feeding-related genes and in particular those related to salivary proteins. In general, these transcriptome alterations targeted pathways that seem to be particularly adapted to the transmission mode of the corresponding virus and could be evidence of vector manipulation by the virus.

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“…Interestingly, two of them, forkhead box protein O (Foxo) and ATP-binding cassette sub-family G member 4-like (ABCG4) could be involved in aphid wing formation (Grantham et al, 2020;Shang et al, 2020). Induction of wings could considerably increase virus propagation by aphids, especially over long distances, as recently shown for CMV transmission (Jayasinghe et al, 2021). In this specific case, the wing formation was attributed to a virus satellite co-infecting the plant.…”

Section: Virus-specific Aphid Degs On Both Host-plants 2a Tuyv-specif...mentioning

confidence: 81%

Transcriptome responses of the aphid vectorMyzus persicaeare shaped by identities of the host plant and the virus

Chesnais

Golyaev²,

Velt

et al. 2022

Preprint

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Background: Numerous studies document modifications in vector orientation behavior, settling and feeding behavior, and/or performance due to virus infection in host plants. These alterations are often expected to enhance virus transmission, which has led to the hypothesis that such effects are manipulations caused by virus adaptations. However, until now, the genetic basis of these effects on vectors that can be direct (effects that occur following acquisition and retention of virions) or indirect (plant-mediated effects) are mostly unknown. Results: Transcriptome profiling of Myzus persicae aphids feeding on turnip yellows virus (TuYV) and cauliflower mosaic virus (CaMV) infected Arabidopsis thaliana and Camelina sativa revealed a substantial proportion of commonly deregulated genes, revealing general players in plant-virus-aphid interactions. We identified also aphid genes specifically deregulated by CaMV or TuYV infection, which might be related to the viral transmission mode. Furthermore, we observed strong host-specific differences in the gene expression patterns with plant virus infection causing more deregulations of aphid genes on Arabidopsis than on Camelina, likely related to the differences in susceptibility of the plant hosts to these viruses. Finally, stress-related aphid genes were downregulated in Myzus on both infected plants, regardless of the virus. Conclusions: TuYV - relying on the circulative persistent mode of transmission - tends to affect developmental genes, which could increase the proportion of alate aphids in TuYV viruliferous aphids, but also contribute to their locomotion, neuronal activity, and lifespan, while CaMV - using the non-circulative non-persistent mode of transmission - had a strong impact on feeding-related genes and in particular those related to salivary proteins. In general, these transcriptome alterations target pathways that seem to be particularly adapted to the transmission mode of the corresponding virus and could be evidence of manipulation caused by viral adaptation. Keywords: Caulimovirus, polerovirus, aphid vector, insect-plant interactions, transmission, transcriptome profiling, RNA-seq

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A plant virus satellite RNA directly accelerates wing formation in its insect vector for spread

Cited by 31 publications

References 36 publications

Induction of aphid resistance in tobacco by the cucumber mosaic virus CMV∆2b mutant is jasmonate‐dependent

Induction of aphid resistance in tobacco by the cucumber mosaic virus CMV∆2b mutant is jasmonate‐dependent

Transcriptome responses of the aphid vector Myzus persicae are shaped by identities of the host plant and the virus

Transcriptome responses of the aphid vectorMyzus persicaeare shaped by identities of the host plant and the virus

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