A plaque assay for foot-and-mouth disease virus and kinetics of virus reproduction

Bachrach, Howard L.; Callis, J. J.; Hess, W. R.; Patty, R. E.

doi:10.1016/0042-6822(57)90060-0

Cited by 117 publications

(28 citation statements)

References 7 publications

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“…The presence of Mg and Ca ions in the adsorption medium improved the adsorption rate, coinciding the observations with other virus-cell systems [1,4,5].…”

Section: Discussionsupporting

confidence: 68%

Plaque Formation and Initial Virus‐Cell Interaction of Measles Virus

Arita

Matumoto

1968

Japanese Journal of Microbiology

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The Sugiyama strain of measles virus adapted to FL cells produces plaques heterogeneous in size and morphology on FL and other stable human cell line monolayers making it difficult to assay by the plaque technique. A virus clone producing homogeneous clear plaques was established by repeated cloning of the strain. A practical reliable plaque assay method was established for this cloned line. FL cells were found to be well suited for this purpose, but Vero cells, a stable line of African green monkey kidney cells, were shown to be more sensitive than FL cells. HeLa cells could be used for the assay, but were less sensitive than the Vero or FL cell line. The initial step of measles virus adsorption onto host cells was shown to be reversible, dependent on electrolytes and almost temperature-independent, strongly suggesting adsorption is electrostatic in nature. At 36 C the initial stage of adsorption quickly passed into the next irreversible stage involving a temperature-dependent reaction; virus adsorbed at 36 C soon became resistant to the action of measles antibody, whereas little virus adsorbed at 4 C or 12 C became antibody-resistant. The time required for the adsorbed virus to move into the irreversible, antibody-resistant stage seems very short.

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“…The presence of Mg and Ca ions in the adsorption medium improved the adsorption rate, coinciding the observations with other virus-cell systems [1,4,5].…”

Section: Discussionsupporting

confidence: 68%

Plaque Formation and Initial Virus‐Cell Interaction of Measles Virus

Arita

Matumoto

1968

Japanese Journal of Microbiology

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“…of the tissue culture fluid described by Bachrach et al (1962). The extracts were examined for infectivity by plaque assay (Bachrach et al, 1957). Under these conditions, the recovery of total infectivity from the strips varied from 20 to 40% of the amount applied.…”

Section: In]ectivitymentioning

confidence: 99%

The influence of the host cell, ionic strength and chymotrypsin on foot-and-mouth disease virus electrophoretic mobility

Woude

Trautman

Bachrach

1967

Archiv f Virusforschung

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“…of each virus dilution was placed on primary calf kidney cell cultures, using the prescription bottle method described by Bachrach et al (2) and the modifications previously reported (10,24).…”

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confidence: 99%