“…Protoplast formation, protoplast regeneration and protoplast transformation of Thermo monospora fusca with plasmid pIJ702 has also been reported (Pidcock et al, 1985). In addition, new plasmid cloning vectors, including shuttle vectors, are being constructed and characterized in Streptomyces, as well as in members of other actinomycete genera (Kieser et al, 1982;Katz et al, 1983;Jones et al, 1984;Jaurin andCohen, 1984, 1985;Jones etal., 1984;Larson and Hershberger, 1984;Parag and Goedeke, 1984;Cohen et al, 1985;Hopwood et al, 1985;Ali and Dale, 1986;Bailey et al, 1986;Krugel and Fielder, 1986;Hopwood, 1986;Larson and Herschberger, 1986;Nakanishi et al, 1986;Oshida et al, 1986;Engel, 1987;Hercomb etal, 1987). In addition, new plasmid cloning vectors, including shuttle vectors, are being constructed and characterized in Streptomyces, as well as in members of other actinomycete genera (Kieser et al, 1982;Katz et al, 1983;Jones et al, 1984;Jaurin andCohen, 1984, 1985;Jones etal., 1984;Larson and Hershberger, 1984;Parag and Goedeke, 1984;Cohen et al, 1985;Hopwood et al, 1985;Ali and Dale, 1986;Bailey et al, 1986;Krugel and Fielder, 1986;Hopwood, 1986;Larson and Herschberger, 1986;Nakanishi et al, 1986;Oshida et al, 1986;Engel, 1987;Hercomb etal, 1987).…”