A plasmid vector allowing positive selection of recombinant plasmids in Streptococcus pneumoniae

Prats, Anne-Catherine; Martin, Bernard; Pognonec, Philippe; Burger, Anne-Catherine; Claverys, Jean‐Pierre

doi:10.1016/0378-1119(85)90105-2

Cited by 21 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noteworthy that the majority of reported instability occurrences are observed in attempts to clone eukaryotic DNA in constructs propagated in prokaryotic host (Al-Allaf et al 2005; Boyd et al 1999; Razin et al 2001; Shirsat et al 1992; Wyman et al 1985). Fragments with toxic genes (Vidal et al 1991), a long inverted DNA repeat separated by a short intervening segment (Prats et al 1985), multiple direct repeats (Bichara et al 2000; Kang and Cox 1996), AT-rich sequences and sequences capable of forming cruciform-like structures (Razin et al 2001) or other non-B DNA structures (Catasti et al 1996; Murchie and Lilley 1992; Seigneurin et al 1988; Simonsson et al 1998) are known to contribute to plasmid instability.…”

Section: Discussionmentioning

confidence: 99%

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3

et al. 2012

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3

et al. 2012

View full text Add to dashboard Cite

“…The cells were centrifuged and resuspended in the same medium supplemented with 3 x M NaOH and 0.25 pg Csp ml-' (Havarstein et al, 1995). The replicative plasmid used was pSP2 (Prats et al, 1985a) which carries resistance markers for erythromycin and tetracycline. Transformants were selected after incubation for 2 h at 37 "C on selective plates (1-5 pg tetracycline ml-') to test for plasmid replication in the recipients.…”

Section: Methodsmentioning

confidence: 99%

Electrotransformation and natural transformation of Streptococcus pneumoniae:requirement of DNA processing for recombination

1998

View full text Add to dashboard Cite

Electrotransformation has been used as a tool to introduce genes carried on replicative vectors in hundreds of bacterial species. In this study, the technique was used to try to obtain recombination of markers in the chromosome of the na tura I I y transformable bacterium Streptococcus pneumoniae . Recombination was not observed even using naturally competent cultures. Both chromosomal and cloned DNA, denatured or native, were without effect. These results suggest that it is not sufficient to introduce DNA into the cell to obtain recombinants in this bacterium. The integration of markers into the chromosome in naturally competent cells must require DNA processing during entry. Electrotransformation of replicating plasmids is red-independent but can be facilitated by a red-dependent process. This facilitation required the induction of the natural competence machinery, probably involving partial homologous pairing.

show abstract

“…Our aim was to construct a plasmid cloning vector for corynebacteria in which the cloning sites are flanked by transcriptional terminators by using the principle of the non-viability of plasmids carrying long uninterrupted perfect palindromes (inverted repeats) as it was decribed before in E. coli [22], Streptococcus pneumoniae [23], and Streptomyces [24].…”

Section: Construction Of Pulmj55 a Positive Selection Rector For Cmentioning

confidence: 99%

Construction of new cloning vectors for Brevibacterium lactofermentum

Cadenas

1996

FEMS Microbiology Letters

View full text Add to dashboard Cite

Two plasmid cloning vectors (pULMJ55 and pULMJ95) were constructed for Brevibacterium lactofermentum using the origin of replication of the endogenous plasmid pBL1. Plasmid pULMJ55 is a replacement vector with transcriptional terminators from the B. lactofermentum trp operon flanking the BglII cloning sites. Religation of the BglII digested vector without insert creates a 376 bp perfect palindrome that is not tolerated in B. lactofermentum, giving positive selection for recombinant plasmids with inserts. Plasmid pULMJ95 contains the promoter-less alpha-amylase gene from Streptomyces griseus downstream of the trp terminator and is particularly suitable for the detection of promoters which are activated late during the growth phase. alpha-Amylase is secreted and its activity can be detected using simple plate tests.

show abstract

A plasmid vector allowing positive selection of recombinant plasmids in Streptococcus pneumoniae

Cited by 21 publications

References 21 publications

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3

Electrotransformation and natural transformation of Streptococcus pneumoniae:requirement of DNA processing for recombination

Construction of new cloning vectors for Brevibacterium lactofermentum

Contact Info

Product

Resources

About