A plate of viruses: Viral metagenomics of supermarket chicken, pork and beef from Brazil

Cibulski, Samuel Paulo; Lima, Diane Alves de; Santos, Helton Fernandes dos; Teixeira, Thaís Fumaco; Tochetto, Caroline; Mayer, Fabiana Quoos; Roehe, Paulo Michel

doi:10.1016/j.virol.2020.09.005

Cited by 21 publications

(13 citation statements)

References 56 publications

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“…Many new cressdnaviruses, that are yet to be classified into families, have been discovered over the last decade. Identification of these in poultry samples is no exception [58,59,72]. Here, we have 13 cressdnaviruses that cannot be assigned to any currently established families (Figure 1, Figure 6 and Table 3).…”

Section: Unclassified Cressdnavirusesmentioning

confidence: 93%

Diverse Single-Stranded DNA Viruses Identified in Chicken Buccal Swabs

et al. 2021

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High-throughput sequencing approaches offer the possibility to better understand the complex microbial communities associated with animals. Viral metagenomics has facilitated the discovery and identification of many known and unknown viruses that inhabit mucosal surfaces of the body and has extended our knowledge related to virus diversity. We used metagenomics sequencing of chicken buccal swab samples and identified various small DNA viruses with circular genome organization. Out of 134 putative circular viral-like circular genome sequences, 70 are cressdnaviruses and 26 are microviruses, whilst the remaining 38 most probably represent sub-genomic molecules. The cressdnaviruses found in this study belong to the Circoviridae, Genomoviridae and Smacoviridae families as well as previously described CRESS1 and naryavirus groups. Among these, genomoviruses and smacoviruses were the most prevalent across the samples. Interestingly, we also identified 26 bacteriophages that belong to the Microviridae family, whose members are known to infect enterobacteria.

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Section: Unclassified Cressdnavirusesmentioning

confidence: 93%

Diverse Single-Stranded DNA Viruses Identified in Chicken Buccal Swabs

et al. 2021

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“…Viral genomes and CRESS DNAs have been found in various meat samples in Porto Alegre, Brazil and in San Francisco, USA. However, in these reports the detection was not linked to human diseases [13,14]. In contrast, multiple unclassified circular viral-like DNA elements, isolated from raw milk and cow milk at retail in Germany are suspected to contribute to the emergence of colon and breast cancer and neurodegenerative diseases [5,[15][16][17].…”

Section: Introductionmentioning

confidence: 98%

Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk

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et al. 2021

Viruses

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In recent years, a variety of circular replicase-encoding single-stranded (CRESS) DNA viruses and unclassified virus-like DNA elements have been discovered in a broad range of animal species and environmental samples. Key questions to be answered concern their presence in the human diet and their potential impact on disease emergence. Especially DNA elements termed bovine meat and milk factors (BMMF) are suspected to act as co-factors in the development of colon and breast cancer. To expand our knowledge on the occurrence of these potential pathogens in human nutrition, a total of 73 sheep and 40 goat milk samples were assayed by combining rolling circle amplification (RCA), PCR and Sanger sequencing. The present study further includes retail milk from the aforementioned species. We recovered 15 single stranded (ss) circular genomes. Of those, nine belong to the family Genomoviridae and six are members of the unclassified group of BMMF. Thus, dairy sheep and goats add to dispersal of CRESS viruses and circular ssDNA elements, which enter the food chain via milk. The presence of these entities is therefore more widespread in Bovidae than initially assumed and seems to be part of the common human nutrition.

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“…У м'ясних продуктах із магазинів Сан-Франциско (США) і супермаркетів Порту-Алегрі (південна Бразилія) виявлено методом метагеномного секвенування нуклеотидні послідовності десятків вірусів із різних таксономічних груп, зокрема: в яловичині -з родин Parvoviridae, Anelloviridae, Circoviridae, Polyomaviridae, у свинині -з родин Parvoviridae, Anelloviridae, Circoviridae, Genomoviridae, в курятині -з родин Anelloviridae, Smacoviridae (Zhang et al, 2014;Cibulski et al, 2021). Зокрема, торкутеновіруси свиней із родини Anelloviridae часто виявляються в організмі клінічно здорових свиней і диких кабанів у цілому світі (Teixeira et al, 2013;Ramos et al, 2018).…”

Section: о с калінінаunclassified

Viruses in food products

Kalinina

2021

SMLNUVMB

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Data on viral food contaminants that are actually or potentially capable of realizing the food route of infection are presented. The main sources of infection of food with viruses are named: human waste / faeces, contaminated food processing facilities, animals-carriers of zooanthroponotic infections. The groups of viruses transmitted through food are characterized: 1) gastroenteritis pathogens – Sapporo and Norwalk viruses from the family Caliciviridae; Rotavirus A from the family Reoviridae; Mammastroviruses 1, 6, 8 and 9 from the family Astroviridae; Human mastadenovirus F from the family Adenoviridae; Aichivirus A from the family Picornaviridae; 2) Hepatovirus A from the family Picornaviridae and Orthohepevirus A from the family Hepeviridae (with replication in the liver); 3) viruses with replication in the human intestine, which after generalization of the infection affect the CNS – Еnteroviruses B and C from the family Picornaviridae. The stability and survival time of viruses in the environment and food are shown. The main ways of transmission of viruses that are able to enter the human body through infected foods are considered. Influenza A (H1N1) virus has been identified as a possible contaminant in pork and chicken, which without heat treatment can pose a potential risk of human infection. The ability of classical and African swine fever pathogens to remain viable after industrial processing of meat or raw meat has been shown. Families of viruses whose zoopathogenic representatives can contaminate meat products (beef, pork, chicken) are named: Parvoviridae, Anelloviridae, Circoviridae, Polyomaviridae, Smacoviridae. To determine the possible latent infection of people with these viruses, it is necessary to test sera for the presence of specific antibodies. The detection of gyroviruses of the family Anelloviridae and huchismacoviruses of the family Smacoviridae in human faeces may be due to the consumption of infected chicken meat. Data on extraction and concentration methods and methods of virus detection in contaminated food products: PCR (reverse transcription and real-time), ELISA, IСA, electron microscopy, virus isolation in transplanted cell cultures with subsequent identification in serological reactions, NR, IFА, ELISA) or PCR.

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A plate of viruses: Viral metagenomics of supermarket chicken, pork and beef from Brazil

Cited by 21 publications

References 56 publications

Diverse Single-Stranded DNA Viruses Identified in Chicken Buccal Swabs

Diverse Single-Stranded DNA Viruses Identified in Chicken Buccal Swabs

Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk

Viruses in food products

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