A Podospora anserina longevity mutant with a temperature-sensitive phenotype for senescence.

Abstract

A Podospora anserina longevity mutant was identified with a temperature-sensitive phenotype for senescence. This mutant, termed TS1, grew for over 3 m at 27°C, but when shifted to 34°C, it underwent senescence between 10 and 18 cm. A previously described senescence-associated plasmid, a senDNA, derived from the mitochondrial genome, was not detected in TS1 at 27°C but was present in senescent cultures at 34°C. A similar result was observed in progeny strains obtained by crossing the TS1 mutant with a wild-type strain. Other mitochondrial excision-amplification DNAs in addition to a senDNA were also observed in the senescent cultures. Most were derived from a specific region of the mitochondrial genome. These results provide evidence that asenDNA is involved in TS1 senescence and suggest that this plasmid may play a role in the formation of other mitochondrial excision-amplification plasmids.

Genome-Scale Studies of Aging: Challenges and Opportunities

“…Aging in the relatively simple eukaryote budding yeast Saccharomyces cerevisiae has been extensively studied and is complemented by research in other fungi, including Schizosaccharomyces pombe [ 18 , 19 ], Podospora anserina [ 20 - 22 ], and Neurospora crassa [ 23 ]. S. cerevisiae lifespan has been measured in at least two ways.…”

Changes in growth competence of aged Trichoderma viride vegetative mycelia

“…This fungus displays several characteristic changes on the morphological and genetic levels, the senescence occurs in all known isolates and could be postponed in several mutants (Silliker and Cummings 1990;Tudzynski and Esser 1979;Turker et al 1987). Evidence were accumulated that mitochondrial genes and functions are involved in senescence (Osiewacz 2000a, b).…”