A poly-l-lysine-bonded TEMPO-oxidized bacterial nanocellulose-based antibacterial dressing for infected wound treatment

Shahriari-Khalaji, Mina; Li, Geli; Liu, Lu; Sattar, Mamoona; Chen, Lin; Zhong, Chunyan; Hong, Feng

doi:10.1016/j.carbpol.2022.119266

Cited by 54 publications

(21 citation statements)

References 52 publications

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“…In this study, L929 mouse murine fibroblast and HUVEC cells are used to evaluate the potential of the scaffolds in keeping cells alive 52 , 53 . Before culturing the cells, the scaffolds are sterilized using ethanol and ultra-violet (UV) light.…”

Section: Methodsmentioning

confidence: 99%

Melt electrowriting of PLA, PCL, and composite PLA/PCL scaffolds for tissue engineering application

Shahverdi

Seifi

Akbari

et al. 2022

Sci Rep

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Fabrication of well-ordered and bio-mimetic scaffolds is one of the most important research lines in tissue engineering. Different techniques have been utilized to achieve this goal, however, each method has its own disadvantages. Recently, melt electrowriting (MEW) as a technique for fabrication of well-organized scaffolds has attracted the researchers’ attention due to simultaneous use of principles of additive manufacturing and electrohydrodynamic phenomena. In previous research studies, polycaprolactone (PCL) has been mostly used in MEW process. PCL is a biocompatible polymer with characteristics that make it easy to fabricate well-arranged structures using MEW device. However, the mechanical properties of PCL are not favorable for applications like bone tissue engineering. Furthermore, it is of vital importance to demonstrate the capability of MEW technique for processing a broad range of polymers. To address aforementioned problems, in this study, three ten-layered box-structured well-ordered scaffolds, including neat PLA, neat PCL, and PLA/PCL composite are fabricated using an MEW device. Printing of the composite PLA/PCL scaffold using the MEW device is conducted in this study for the first time. The MEW device used in this study is a commercial fused deposition modeling (FDM) 3D printer which with some changes in its setup and configuration becomes prepared for being used as an MEW device. Since in most of previous studies, a setup has been designed and built for MEW process, the use of the FDM device can be considered as one of the novelties of this research. The printing parameters are adjusted in a way that scaffolds with nearly equal pore sizes in the range of 140 µm to 150 µm are fabricated. However, PCL fibers are mostly narrower (diameters in the range of 5 µm to 15 µm) than PLA fibers with diameters between 15 and 25 µm. Unlike the MEW process of PCL, accurate positioning of PLA fibers is difficult which can be due to higher viscosity of PLA melt compared to PCL melt. The printed composite PLA/PCL scaffold possesses a well-ordered box structure with improved mechanical properties and cell-scaffold interactions compared to both neat PLA and PCL scaffolds. Besides, the composite scaffold exhibits a higher swelling ratio than the neat PCL scaffold which can be related to the presence of less hydrophobic PLA fibers. This scaffold demonstrates an anisotropic behavior during uniaxial tensile test in which its Young’s modulus, ultimate tensile stress, and strain to failure all depend on the direction of the applied tensile force. This anisotropy makes the composite PLA/PCL scaffold an exciting candidate for applications in heart tissue engineering. The results of in-vitro cell viability test using L929 mouse murine fibroblast and human umbilical vein endothelial (HUVEC) cells demonstrate that all of the printed scaffolds are biocompatible. In particular, the composite scaffold presents the highest cell viability value among the fabricated scaffolds. All in all, the composite PLA/PCL scaffold shows that it can be a promising substitution for neat PCL scaffold used in previous MEW studies.

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Section: Methodsmentioning

confidence: 99%

Melt electrowriting of PLA, PCL, and composite PLA/PCL scaffolds for tissue engineering application

Shahverdi

Seifi

Akbari

et al. 2022

Sci Rep

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“…The S. aureu s-infected dermal wound model was established on the back of male Sprague Dawley rats to evaluate the healing effect of collagen/ε-polylysine nanocomposite fibrils for infected wounds. 45 Male rats (200 g–250 g) were purchased from Chengdu Dashuo Experimental Animal Co., Ltd, China. A round-shaped full-thickness wound with a diameter of ∼10 mm was created on the dorsal region of each rat after anesthesia.…”

Section: Methodsmentioning

confidence: 99%

An antibacterial and healing-promoting collagen fibril constructed by the simultaneous strategy of fibril reconstitution and ε-polylysine anchoring for infected wound repair

Zhang,

Yang,

Guo

et al. 2023

Biomater. Sci.

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“…Subsequently, the obtained samples were washed with ethanol and water and kept at 4 °C for further use. 20 …”

Section: Methodsmentioning

confidence: 99%

Functionalization of aminoalkylsilane-grafted cotton for antibacterial, thermal, and wettability properties

et al. 2022

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A poly-l-lysine-bonded TEMPO-oxidized bacterial nanocellulose-based antibacterial dressing for infected wound treatment

Cited by 54 publications

References 52 publications

Melt electrowriting of PLA, PCL, and composite PLA/PCL scaffolds for tissue engineering application

Melt electrowriting of PLA, PCL, and composite PLA/PCL scaffolds for tissue engineering application

An antibacterial and healing-promoting collagen fibril constructed by the simultaneous strategy of fibril reconstitution and ε-polylysine anchoring for infected wound repair

Functionalization of aminoalkylsilane-grafted cotton for antibacterial, thermal, and wettability properties

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