Fabrication of well-ordered and bio-mimetic scaffolds is one of the most important research lines in tissue engineering. Different techniques have been utilized to achieve this goal, however, each method has its own disadvantages. Recently, melt electrowriting (MEW) as a technique for fabrication of well-organized scaffolds has attracted the researchers’ attention due to simultaneous use of principles of additive manufacturing and electrohydrodynamic phenomena. In previous research studies, polycaprolactone (PCL) has been mostly used in MEW process. PCL is a biocompatible polymer with characteristics that make it easy to fabricate well-arranged structures using MEW device. However, the mechanical properties of PCL are not favorable for applications like bone tissue engineering. Furthermore, it is of vital importance to demonstrate the capability of MEW technique for processing a broad range of polymers. To address aforementioned problems, in this study, three ten-layered box-structured well-ordered scaffolds, including neat PLA, neat PCL, and PLA/PCL composite are fabricated using an MEW device. Printing of the composite PLA/PCL scaffold using the MEW device is conducted in this study for the first time. The MEW device used in this study is a commercial fused deposition modeling (FDM) 3D printer which with some changes in its setup and configuration becomes prepared for being used as an MEW device. Since in most of previous studies, a setup has been designed and built for MEW process, the use of the FDM device can be considered as one of the novelties of this research. The printing parameters are adjusted in a way that scaffolds with nearly equal pore sizes in the range of 140 µm to 150 µm are fabricated. However, PCL fibers are mostly narrower (diameters in the range of 5 µm to 15 µm) than PLA fibers with diameters between 15 and 25 µm. Unlike the MEW process of PCL, accurate positioning of PLA fibers is difficult which can be due to higher viscosity of PLA melt compared to PCL melt. The printed composite PLA/PCL scaffold possesses a well-ordered box structure with improved mechanical properties and cell-scaffold interactions compared to both neat PLA and PCL scaffolds. Besides, the composite scaffold exhibits a higher swelling ratio than the neat PCL scaffold which can be related to the presence of less hydrophobic PLA fibers. This scaffold demonstrates an anisotropic behavior during uniaxial tensile test in which its Young’s modulus, ultimate tensile stress, and strain to failure all depend on the direction of the applied tensile force. This anisotropy makes the composite PLA/PCL scaffold an exciting candidate for applications in heart tissue engineering. The results of in-vitro cell viability test using L929 mouse murine fibroblast and human umbilical vein endothelial (HUVEC) cells demonstrate that all of the printed scaffolds are biocompatible. In particular, the composite scaffold presents the highest cell viability value among the fabricated scaffolds. All in all, the composite PLA/PCL scaffold shows that it can be a promising substitution for neat PCL scaffold used in previous MEW studies.

In the current study, the regime of motion of fullerene molecules on substrates with different shapes at a range of specific temperatures has been investigated. To do so, the potential energy of fullerene molecules was analyzed using the classical molecular dynamics method. C20, C36, C50, C60, C72, C76, C80, and C90 fullerene molecules were selected due to their spherical shapes with different sizes. In addition, to completely analyze the behavior of these molecules, different gold substrates, including flat, concave, the top side of the step (upward step), and the downside of the step (downward step) substrates, were considered. Specifying the regime of the motion at different temperatures is one of the main goals of this study. For this purpose, we have studied the translational and rotational motions of fullerene molecules independently. In the first step of the investigation, Lennard-Jones potential energy of fullerene molecules was calculated. Subsequently, the regime of motion of different fullerenes has been classified, based on their displacement and sliding velocity. Our findings indicated that C60 is appropriate in less than $$5\%$$ 5 % of the conditions. However, C20, C76 and C80 molecules were found to be appropriate candidates in most cases in different conditions while they were incompetent only in seven situations. As far as a straight-line movement is considered, the concave geometry demonstrated a better performance compared to the other substrates. In addition, C72 indicated less favorable performance concerning the range of movement and diffusion coefficients. All in all, our investigation helps to understand the performance of different fullerene molecules on gold substrates and find their probable application, especially as a wheel in nano-machine structures.

The current study presents one of the first investigations in which the simultaneous effect of the curved gold substrates and temperature changes on C60 and C60-wheeled nano-machines’ migration was evaluated. For this aim, the cylindrical and concave substrates with different radii were chosen to attain the size of the most appropriate substrate for nano-machines. Results indicated that the chassis' flexibility substantially affected the nanocar's mobility. Nano-machines' deviation from their desired direction was adequately restricted due to selected substrate geometries (The cylindrical and concave). Besides, for the first time, the effect of the substrate radius changes on nano-machine's motion has been investigated. Our findings revealed that adjusting the value of radius results in a long-range movement for nano-machines as well as a sufficient amount of diffusion coefficient even at low temperatures ($$75\; \text{K}$$ 75 K or $$150\; \text{K}$$ 150 K ). As a result, the aforementioned substrates could be utilized as the optimized geometries for C60 and nanocar at all temperatures. At the same time, the nanotruck displayed an appropriate performance merely on the small cylindrical substrate ($$radius=17.5 \; \text{\AA} $$ r a d i u s = 17.5 Å ) at high temperatures ($$500\; \text{K}$$ 500 K and $$600\; \text{K}$$ 600 K ).

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