A polymer-based DNA biochip platform for human papilloma virus genotyping

Brandstëtter, Thomas; Böhmer, Sebastian; Prucker, Oswald; Bissé, Emmanuel; Hausen, Axel zur; Alt-Mörbe, Juliane; Rühe, Jürgen

doi:10.1016/j.jviromet.2009.07.027

Cited by 44 publications

(41 citation statements)

References 30 publications

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“…A polymer-based biochip was recently developed to distinguish between the 12 most common HPV genotypes [29]. 36 DNA probes were chosen and printed with polydimethylacrylamide (PDMAA) on a substrate in a microarray pattern via a UV-irradiation process.…”

Section: A Optical Detection Technologiesmentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

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| Human papillomavirus (HPV) has been shown in many studies as a prerequisite for the development of cervical cancer, which is the second most common and predominant form of malignancy among women worldwide, with 270 000 deaths (80% of whom live in developing countries) and 500 000 new cases per year. Early diagnosis of cervical cancer allows patients to be fully treated. Therefore, there is high clinical utility of HPV diagnostics even with binary positive/ negative indication of the presence of HPV in patient samples.Although the Pap smear is widely used for cervical cancer screening, this method still suffers from low sensitivity and specificity. Thus, simple, rapid and inexpensive diagnostic methods are needed to detect the etiology of cervical cancer, i.e., HPV, especially high-risk oncogenic subtypes 16 and 18.Here, we review the existing assays and platforms employed for HPV diagnosis, and highlight recent advances in nanotechnology and microfluidics that potentially enable new approaches for HPV diagnosis.

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Section: A Optical Detection Technologiesmentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

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“…Moreover, microarray is much more sensitive (100%) as compared to conventional methods (Brandstetter et al, 2010;Geraets et al, 2009). …”

Section: Microarraymentioning

confidence: 99%

A cornucopia of screening and diagnostic techniques for human papillomavirus associated cervical carcinomas

Ramzan

Ain

Ilyas

et al. 2015

Journal of Virological Methods

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“…From demands of routine clinical requirements, faster methods and those that do not need sophisticated equipment and data management are preferable. Several low-to medium-density arrays, based on the hybridization of labeled target DNA with capture probe with fluorescent [133,134] or colorimetric detection [135,136], have been developed for HPV genotyping. Another method was based on multiplex amplification of the viral L1 region with improved primers and subsequent ligation reaction of two adjacently hybridized ssDNA probes [137].…”

Section: Commercial Hpv Testsmentioning

confidence: 99%