A Polymorphism in the <b><i>MTRR</i></b> Gene Is Associated with Early Childhood Caries and Underweight

Abstract

Polymorphisms in genes encoding the enzymes involved in the metabolism of homocysteine, such as methionine synthase (MTR) and methionine synthase reductase (MTRR), play an important function in the metabolism of folic acid and vitamin B₁₂. The present study aimed to evaluate the association of polymorphisms in genes MTR (rs1805087) and MTRR (rs1801394) with susceptibility of early childhood caries (ECC) and with body mass index alterations. A cross-sectional study was performed in 488 children aged from 2 to 6 years from 25 public day care centers in Rio de Janeiro, Brazil. Demographic data and oral health habits were obtained through a questionnaire. Anthropometric measurements and caries experience data were collected by 2 examiners (κ = 0.80). Genotyping of the selected polymorphisms was carried out by TaqMan real-time PCR using genomic DNA extracted from buccal cells. Allele and genotype frequencies were compared between groups with and without disease. The t test, χ² test, odds ratio, Pearson correlation tests, and logistic regression analysis were used (p ≤ 0.05). The mean white spot lesion score was 1.18 (±2.57) in normal weight children and 2.50 (±3.87) in underweight children (p = 0.05). For MTRR polymorphisms, significant differences were observed for allele and genotype frequency distributions between caries-free and caries-affected children (p = 0.03 and 0.04 for allele and genotype frequencies, respectively) and in the genotype frequencies between normal weight and underweight children (p = 0.04). Our results suggest an association between underweight and ECC; in addition it is suggested that MTRR is a common genetic risk factor for ECC and underweight.

Interaction of vitamin D, homocystein and dental diseases

“…Вивчені поліморфізми в генах МТRR, що кодує фермент метіонін синтазу редуктази і МТR (кодує метіонін синтазу) [23], які беруть участь у метаболізмі гомоцистеїну та відіграють важливу роль в обміні фолієвої кислоти та вітаміну В 12 . Так, алельні зміни в гені МТRR тісно асоціюються з виникненням гострого початкового карієсу тимчасових зубів, а зміни в гені МТR мають сумнівний взаємозв'язок із каріозним процесом [9].…”

Dental genetics in Brazil: Where we are

“… Polymorphisms in nonamelogenin enamel matrix genes are associated with dental fluorosis (Küchler et al., ) MUC5B gene is associated with dental caries (Cavallari, Salomão, Moysés, Moysés, & Werneck, ) Genes involved in the enamel development are associated with calcium and phosphorus level in saliva (Küchler et al., ) Polymorphisms in DEFB1 and miRNA202 are involved in salivary human β‐defensin 1 levels and caries experience in children (Lips et al., ) A polymorphism in the MTRR gene is associated with early childhood caries and underweight (Antunes et al., ) Aquaporin 5 interacts with fluoride and possibly protects against caries (Anjomshoaa et al., ) MMP2 , MMP3 , MMP9 , MMP20 , TIMP1 , and TIMP2 polymorphisms are associated with white spot lesions and early childhood caries (Antunes et al., ) Genetic influences on dental enamel that impact caries differ between the primary and permanent dentitions (Bayram et al., ) BMP2 gene modification is associated with caries experience in primary teeth (Romanos et al., ) …”

Effect of Experimental Hypergomocysteinemia and its Correction by Choline and Vitamin D on the Reproductive Function and Dental Status of Rates of Rats

“…ЕКСПЕРИМЕНТАЛЬНА ТА КЛІНІЧНА ФІЗІОЛОГІЯ І БІОХІМІЯ" "EXPERIMENTAL AND CLINICAL PHYSIOLOGY AND BIOCHEMISTRY" Науково-практичний журнал/Scientific-practical journal Наукові статті / Research Articles ECPB 2019, 3 Гіпергомоцистеїнемія (ГГЦ), що виникає при підвищеному рівні гомоцистеїну (ГЦ) плазми, а також недостатність вітаміну D негативно впливають на фізіологічні процеси в організмі людини та стоматологічне здоров'я [1][2][3][4][5][6][7]. В окремих дослідженнях показано, що ГГЦ асоціюється з недостатністю вітаміну D [8][9][10][11] і є незалежним чинником розвитку ускладнень вагітності [12][13][14].…”